Jem Biotech Inc.是一家综合性学科,提供生物技术和化学领域的广泛服务。我们的主要目标是成为您的研究合作伙伴。我们是印度Microtest Innovations的主要合作伙伴。
除了我们自己的实力,包括经验丰富的专业科学家,技术人员和先进的基础设施,我们还与印度的其他三个主要研发团队合作。我们在这个行业工作了12年,我们的一些科学家拥有超过25年的经验。
根据客户要求,我们提供负责项目的科学家和技术人员的简历,客户可以直接与项目协调员沟通。一切都是严格保密的。
生物学和生物技术
· 克隆,表达和大规模纯化
· 敲除构建体,酵母遗传学
分析开发
· 基于体外Ag-Ab的测定
· 基于细胞的分析
· 代谢紊乱
· 酶
· 炎症
· 癌症
· 中枢神经系统(CNS)
· 心血管
PAP(焦磷酸解 -活化聚合)/ KlenTaq -S
PAP是一种*的DNA扩增方法(Liu和Sommer:BioTechniques,29 [2000],1072-1080; Nucleic Acids Res,30 [2002],598-604),其中焦磷酸解 和聚合通过DNA聚合酶使用3连续偶联。 '封闭的底漆。 焦磷酸解是DNA聚合的逆反应,其中在无机焦磷酸盐存在下酶除去引物模板双链体3'末端的封闭核苷酸(通常是双脱氧)。在通过焦磷酸解作用除去封闭的核苷酸后,然后可以通过DNA聚合延长活化的引物。
由于PAP 的高特异性(1 / 3×10 11) ,该过程正成为 在大量过量野生型等位基因存在下检测稀有突变,大杂合缺失,基因重复等的有力工具(Liu et al:Biotechniques,40 [2006],661-668)。理论上,PAP可以检测3×10 11拷贝的野生型等位基因中的单个碱基突变。
PAP可以被用来作为一种替代或证实的实时PCR (Boon等人: Prenat DIAGN 27 [2007],932-937)
KlenTaq -S和PAP反应。
已经发现KlenTaq -S是 PAP反应中许多研究小组的合适的热稳定 Taq DNA聚合酶。
KlenTaq -S由该基因的5'缺失和F667Y(Tabor和Richardson)突变表达,编码Thermus aquaticus DNA聚合酶。反复暴露于98 0在PR1反应C缓冲区的酶不显示活性的任何显着损失。
KlenTaq – S的浓度: 10U /μl
建议的PAP反应条件(25μl ):
50 mM Tris-HCl,7.8,16 mM硫酸铵,3.5 mM 氯化镁, 25μM,每种四种dNTP,每种引物0.1μM,5-200 ng 模板,90μM无机焦磷酸盐(Na 4 PPi),2%二甲基亚砜(DMSO) ),2.5 U KlenTaq -S。
骑行条件:
95 0 Ç 为 1分钟(变性),然后
94 0 C持续15秒
60 0 C持续30秒
64 0 C持续30秒
68 ° C,1分钟
72 0 C持续1-2分钟,共25-45个循环
提供缓冲液 ( 10X PR1):
500 毫米 的Tris-HCl,pH值7.8,160 mM的 ( NH 4)2 SO 4,35 毫的MgCl 2,1.5毫克/毫升BSA
订购信息:
订货号 :KTS-01
数量: 100μl或1000 U /小瓶
价格: $ 200 /小瓶(1000 U)
运费: 美国35美元(FedEx隔夜)
Jembio 产品目录:
货号 |
品名 |
规格 |
美金价 |
品牌 |
LA001 |
Acid Phosphatase, Polyclonal anti-Human Affinity Purified |
5 mg |
1300 |
jembio |
LA002 |
Albumin, Polyclonal anti-Human Affinity Purified (ALB) |
10 mg |
800 |
jembio |
LA003 |
Albumin, Polyclonal anti-Human Affinity Purified (ALB) |
10 mg |
600 |
jembio |
LA004 |
Alpha Amylase, Polyclonal anti-Human (whole antiserum) |
50 ml |
900 |
jembio |
LA005 |
Alpha Fetoprotein, Polyclonal anti-Human (AFP) Whole Antiserum |
50 ml |
900 |
jembio |
LA006 |
Alpha-1 Antitrypsin, Polyclonal anti-Human Affinity Purified (A1AT) |
10 mg |
700 |
jembio |
LA007 |
Alpha-1 Antitrypsin, Polyclonal anti-Human Affinity Purified (A1AT) |
10 mg |
700 |
jembio |
LA008 |
Alpha-1-Acid Glycoprotein, anti-Human Affinity Purified (A1AG) |
10 mg |
750 |
jembio |
LA009 |
alpha-2-Macroglobulin, Polyclonal anti-Human affinity Purified (A2M) |
10 mg |
700 |
jembio |
LA010 |
anti-Feline Leukemia Virus p27, Monoclonal |
5 mg |
3000 |
jembio |
LA011 |
anti-Feline Leukemia Virus p27, Monoclonal-CMI |
5mg |
3000 |
jembio |
LA012 |
beta 2-Microglobulin ,anti-Human HRP Conjugated |
10 mg |
1400 |
jembio |
LA013 |
beta 2-Microglobulin, Polyclonal anti-Human Affinity Purified (B2M) |
10 mg |
650 |
jembio |
LA014 |
BNP Polyclonal anti-human affinity purified |
5 mg |
3000 |
jembio |
LA015 |
C-Reactive Protein (CRP), Polyclonal anti-Rat affinity purified |
1 mg |
1500 |
jembio |
LA016 |
C-Reactive Protein, Polyclonal anti-Human affinity purified (CRP) |
10 mg |
750 |
jembio |
LA017 |
C3, Polyclonal anti-Human Affinity Purified |
10 mg |
750 |
jembio |
LA018 |
C4 , Polyclonal anti-Human Affinity Purified |
10 mg |
750 |
jembio |
LA019 |
Cathepsin, Polyclonal anti-Human Affinity Purified |
5 mg |
1450 |
jembio |
LA020 |
Ceruloplasmin, Polyclonal anti-Human Affinity Purified |
10 mg |
750 |
jembio |
LA021 |
Elastase, anti-Human IgG Fraction |
100 mg |
500 |
jembio |
LA021 |
Elastase, anti-Human IgG Fraction |
100 mg |
500 |
jembio |
LA022 |
Elastase, Polyclonal anti-human (whole anti-serum) |
30 ml |
400 |
jembio |
LA023 |
Ferritin, Polyclonal anti-Human Affinity Purified |
10mg |
1750 |
jembio |
LA024 |
Goat anti-Human Whole Serum |
100 ml |
1750 |
jembio |
LA025 |
Haptoglobin Polyclonal anti-human (whole antiserum) |
10 ml |
500 |
jembio |
LA026 |
Haptoglobin, Polyclonal anti-Human Affinity Purified (Hp) |
10 mg |
1200 |
jembio |
LA027 |
Haptoglobin, Polyclonal anti-Human Affinity Purified (Hp) |
10 mg |
1200 |
jembio |
LA028 |
Heartworm,.Polyclonal anti-Canine Affinity Purified |
10 mg |
3000 |
jembio |
LA029 |
Heartworm,Polyclonal anti-Canine,HRP Conjugated |
10 mg |
3500 |
jembio |
LA030 |
Hemoglobin, Polyclonal anti-Human Affinity Purified |
30 ml |
750 |
jembio |
LA031 |
Hemoglobin, Polyclonal anti-Human Affinity Purified |
10 mg |
750 |
jembio |
LA032 |
IgA, Polyclonal anti-Human Affinity Purified |
10 mg |
750 |
jembio |
LA033 |
Kappa, Polyclonal anti-Human Affinity Purified |
10 mg |
1000 |
jembio |
LA034 |
Kappa, Polyclonal anti-Human Affinity Purified |
10 mg |
750 |
jembio |
LA035 |
Lactate Dehydrogenase , Polyclonal anti-Human Affinity Purified (LDH) |
10 mg |
3500 |
jembio |
LA036 |
Lactoferrin Polyclonal anti-Human Affinity Purified |
10 mg |
1300 |
jembio |
LA037 |
Lactoferrin, Polyclonal Whole anti-serum |
50 ml |
1000 |
jembio |
LA038 |
Lambda, Polyclonal anti-Human Affinity Purified |
10 mg |
1000 |
jembio |
LA039 |
Lambda, Polyclonal anti-Human Affinity Purified |
10 mg |
750 |
jembio |
LA040 |
Myeloperoxidase, Polyclonal anti-human affinity purified |
10 mg |
3500 |
jembio |
LA041 |
Myeloperoxidase, Polyclonal anti-Human affinity purified (MPO) |
10 mg |
3500 |
jembio |
LA042 |
Myoglobin Polyclonal anti-human affinity purified |
10 mg |
2500 |
jembio |
LA043 |
Myoglobin, Polyclonal anti-Human Affinity Purified |
10 mg |
5000 |
jembio |
LA044 |
Prostate Specific Antigen,(PSA) Polyclonal anti-Human Affinity Purified |
5 mg |
2300 |
jembio |
LA045 |
Rabbit anti-Human Whole Serum |
50 ml |
2000 |
jembio |
LA046 |
Retinol Binding Protein ,Polyclonal anti-Human Affinity Purified (RBP) |
10 mg |
3200 |
jembio |
LA047 |
Transferrin, Polyclonal anti-Human Affinity Purified |
10 mg |
750 |
jembio |
LA048 |
Troponin I, anti-human whole antiserum |
500 ml |
15000 |
jembio |
1001 |
KlenTaq1TM |
2,500 |
200 |
jembio |
1001 |
KlenTaq1TM |
25,000 |
1,800.00 |
jembio |
1002 |
DNA Polymerase, alpha |
50 |
200 |
jembio |
|
(Calf thymus) |
200 |
725 |
jembio |
1004 |
DNA Polymerase, alpha |
50 |
250 |
jembio |
|
(Human) |
200 |
900 |
jembio |
1006 |
DNA Polymerase, gamma |
50 |
260 |
jembio |
|
(Human) |
200 |
900 |
jembio |
1008 |
DNA Polymerase 1 |
500 |
70 |
jembio |
|
|
2,500 |
280 |
jembio |
1010 |
Klenow Fragment |
1,000 |
280 |
jembio |
|
(DNA Polymerase) |
5,000 |
875 |
jembio |
1011 |
Klenow Fragment |
1,000 |
280 |
jembio |
|
(exo–) |
5,000 |
880 |
jembio |
1012 |
T4 DNA Polymerase |
1,000 |
400 |
jembio |
|
|
2,500 |
835 |
jembio |
1022 |
Terminal Deoxynucleotidyl |
500 |
100 |
jembio |
|
Transferase |
2,500 |
450 |
jembio |
1102 |
AMV Reverse Transcriptase |
500 |
130 |
jembio |
|
|
2,000 |
450 |
jembio |
1104 |
HIV Reverse Transcriptase |
500 |
420 |
jembio |
|
|
2,500 |
1,340.00 |
jembio |
1106 |
Tth 111 Reverse Transcriptase |
100 |
75 |
jembio |
|
|
500 |
300 |
jembio |
1202 |
DNA Ligase, E.coli |
500 |
120 |
jembio |
|
|
2,500 |
500 |
jembio |
1204 |
T4 DNA Ligase |
200 |
75 |
jembio |
|
|
2,000 |
300 |
jembio |
1206 |
Taq DNA Ligase |
1,000 |
110 |
jembio |
|
|
5,000 |
450 |
jembio |
1302 |
Alkaline Phosphatase |
1,000 |
65 |
jembio |
|
|
5,000 |
275 |
jembio |
1304 |
Exonuclease III |
5,000 |
30 |
jembio |
|
|
2,500 |
90 |
jembio |
1306 |
Mung Bean Nuclease |
2,000 |
50 |
jembio |
|
|
10,000 |
200 |
jembio |
1308 |
RNase I |
10,000 |
150 |
jembio |
|
|
25,000 |
300 |
jembio |
1312 |
S1 Nuclease |
10,000 |
65 |
jembio |
|
|
50,000 |
275 |
jembio |
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上海金畔生物科技有限公司是实验试剂一站式采购服务商
1:强大的进口辐射能力,血清、抗体、耗材、大部分限制进口品等。
2:产品种类齐全,经营超过700多品牌,基本涵盖所有生物实验试剂耗材。
3:提供加急服务,货品一般1-2周到货。
4:富有竞争力的价格优势,绝大部分价格有优势。
5:多年积累良好的信誉,大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校,ROCHE,阿斯利康、国药、fisher等知药企。
6:我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等几十家国外公司代理。
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