ZeptoMetrix公司0801198试剂盒说明书

 

zeptometrix成立于1999年,是一家为药物研究行业提供诊断产品和服务的的私人控股公司。zeptometrix公司(ZMC)是一个传染病诊断行业先进者和创新者,为的客户提供值得信赖的产品和服务。zeptometrix是一个*集成的生物技术公司的产品支持的研发的所有阶段;发展,验证,制和商业化的诊断测试。zeptometrix不断开拓创新,致力为生命科学行业带来更多产品与服务。

 

Catalog #: 0801198

Bovine IgG ELISA

 

Preparation of Bovine IgG Standards

Tube

Number

Concentration of

Bovine IgG

Volume of Bovine IgG

Standard

Volume of

Assay Diluent

1

125 ng/mL

1000 ml

0 ml

2

62.5 ng/mL

500 ml of #1

500 ml

3

31.25 ng/mL

500 ml of #2

500 ml

4

15.6 ng/mL

500 ml of #3

500 ml

5

7.8 ng/mL

500 ml of #4

500 ml

6

0 ng/mL

0 ml

500 ml

 

 

 

试验过程

使用前允许所有试剂达到室温。用于制备标准和样品的标号试管。如果不使用整个96井板,则取下剩余的井板。 从板框和放置到重新密封塑料袋与干燥剂。密封袋并存放在2-8°C。

 

步骤1:将每条标签贴在其末端标签上,以确保在检测过程中这些条带与板框分离时的标识。

 

步骤2:将标准1-6的200ml移入重复井.

 

步骤3:每个样品的移液管200 L成重复的威尔斯。

 

第四步:用盖板盖上盖板,在37°C下孵育30分钟。

 

第五步:抽取每口井的内容物,用1x板冲洗缓冲液冲洗4次。清洗时,用1倍平板冲洗缓冲液的300ml注满井,然后抽吸。执行4次填充/吸气循环 s.在后的清洗周期之后,仔细地在吸水性纸巾的垫子上仔细地敲打盘子,*地擦拭盘子。继续,直到没有观察到可见的液滴板洗缓冲液。

 

步骤6:将检测抗体的100m1移入各标准标本中。

 

不要将检测器抗体添加到底物空白井中。

 

步骤7:用盖板盖上盖板,在37°C下孵育30分钟。

 

步骤8:如步骤6所述,用板洗缓冲器冲洗4次。

 

步骤9:将基材的100m1移入每口井,包括基板空白井。

 

步骤10:室温下将盘子孵育30分钟。含有牛IgG的井中会出现蓝色。

 

步骤11:将100m1的止住溶液注入每口井。将发生从蓝色到黄色的颜色变化。

 

步骤12:在15分钟内,用微滴度平板阅读器读取450 nm处每口井的光密度。

 

试样稀释

 

牛初乳通常含有50~80 mg/mL的IgG抗体。1:1,000,000牛初乳稀释在试验稀释剂中被推荐用于初步测试。

 

牛乳通常含有0.5mg/mL的IgG抗体。建议在试验稀释液中稀释1:10,000的牛乳进行初步测试。

 

牛血清通常含有25-30mg/mL的IgG抗体。建议在试验稀释剂中稀释1:500,000牛血清进行初步测试。

 

程序流程图

 

制备试剂稀释剂

吸管标本和标准

37℃孵育30分钟

 

制水板

移液管检测器抗体

37℃孵育30分钟

 

 

制水板

 

移液管底液

室温孵育30分钟

 

 

添加停止溶液并在450 nm处读取

 

 

 

贮存

 

在2-8℃下储存所有试剂盒试剂。

不要冻结。

未使用的微量培养板应保存在带有干燥剂的密封袋中,以使暴露于水分降至低。

储存和处理的所有试剂均为ST 至少可以在包盒标签上打印的过期日期之前。

 

货号

品名

目录价

规格

品牌

0801539DNA-1UG

Cryptococcus Neoformans serotype D, DNA

4524

1 ug

ZeptoMetrix

0801538DNA-1UG

Candida Tropicalis Z012, DNA

4524

1 ug

ZeptoMetrix

0801544DNA-10UG

Proteus Mirabilis Z050, DNA

7001

10 ug

ZeptoMetrix

0801545DNA-10UG

Streptococcus Agalactiae 2603V – R, DNA

7001

10 ug

ZeptoMetrix

0801562DNA-10UG

Bacillus Subtilis Z094, DNA

7001

10 ug

ZeptoMetrix

0801563DNA-10UG

Citrobacter FreundII Z064, DNA

7001

10 ug

ZeptoMetrix

0801569DNA-10UG

Stenotrophomonas Maltophilia Z074, DNA

7001

10 ug

ZeptoMetrix

0801536DNA-1UG

Candida Krusei Z009, DNA

4524

1 ug

ZeptoMetrix

0801537DNA-1UG

Candida Parapsilosis Z011, DNA

4524

1 ug

ZeptoMetrix

0801535DNA-1UG

Candida Glabrata Z007, DNA

4524

1 ug

ZeptoMetrix

0801519DNA-10UG

Pseudomonas Aeruginosa clinical isolate, DNA

7001

10 ug

ZeptoMetrix

0801534DNA-10UG

Listeria Monocytogenes serotype 1 – 2b, DNA

7001

10 ug

ZeptoMetrix

0801518DNA-10UG

Enterobacter Aerogenes Z052, DNA

7001

10 ug

ZeptoMetrix

0801511DNA-10UG

Neisseria Meningitidis serogroup A, DNA

7001

10 ug

ZeptoMetrix

0801506DNA-10UG

Klebsiella Pneumoniae Z026, DNA

7001

10 ug

ZeptoMetrix

0801504DNA-1UG

Candida Albicans Z006, DNA

4524

1 ug

ZeptoMetrix

0801597DNA-10UG

Acinetobacter BaumannII 307-0294, DNA

7001

10 ug

ZeptoMetrix

0801579DNA-10UG

Mycoplasma Pneumoniae M129, DNA

7001

10 ug

ZeptoMetrix

0801679DNA-10UG

Haemophilus Influenzae type b; Eagan, DNA

7001

10 ug

ZeptoMetrix

0801676DNA-1UG

Histoplasma capsulatum Recombinant, DNA

7001

1 ug

ZeptoMetrix

0801677DNA-1UG

Coccidioides Immitis Recombinant, DNA

7001

1 ug

ZeptoMetrix

0801675DNA-10UG

Staphylococcus Aureus MSSA; mecA-deficient, DNA

7001

10 ug

ZeptoMetrix

0801674DNA-10UG

Yersinia Enterocolitica clinical isolate, DNA

7001

10 ug

ZeptoMetrix

0801651DNA-10UG

Staphylococcus Epidermidis MRSE; RP62A, DNA

7001

10 ug

ZeptoMetrix

0801650DNA-10UG

Campylobacter Jejuni clinical isolate, DNA

7001

10 ug

ZeptoMetrix

0801638DNA-10UG

Staphylococcus Aureus MRSA; COL, DNA

7001

10 ug

ZeptoMetrix

0801627DNA-10UG

Shigella Sonnei Z004, DNA

7001

10 ug

ZeptoMetrix

0801624DNA-10UG

Escherichia Coli ETEC; ST+, LT+ DNA

2730

10 ug

ZeptoMetrix

0801622DNA-10UG

Escherichia Coli O157:H7; EDL933, DNA

2730

10 ug

ZeptoMetrix

0801603DNA-1UG

Candida Lusitaniae Z010, DNA

4524

1 ug

ZeptoMetrix

0801619DNA-1UG

Clostridium Difficile NAP1, DNA

7001

1 ug

ZeptoMetrix

0801601DNA-1UG

Aspergillus Terreus Z016, DNA

4524

1 ug

ZeptoMetrix

0801602DNA-1UG

Candida GuilliermondII Z008, DNA

4524

1 ug

ZeptoMetrix

0801598DNA-1UG

Aspergillus Flavus Z013, DNA

4524

1 ug

ZeptoMetrix

0801680DNA-10UG

Haemophilus Influenzae type b; MinnA, DNA

7001

10 ug

ZeptoMetrix

0801482DNA-10UG

Neisseria Gonorrhoeae type strain, DNA

7001

10 ug

ZeptoMetrix

0801439DNA-10UG

Streptococcus Pneumoniae Z022; 19F (Danish), DNA

7001

10 ug

ZeptoMetrix

801223

Goat IgG ELISA

4875

96 det.

ZeptoMetrix

801010

Chicken IgY ELISA

4875

96 det.

ZeptoMetrix

801198

Bovine IgG ELISA

6689

96 det.

ZeptoMetrix

801188

ELISA Construction System

5343

5 plates

ZeptoMetrix

0801185L

ZeptoBlock

3842

1 L

ZeptoMetrix

801185

ZeptoBlock

1697

120 ml

ZeptoMetrix

0801184L

ZeptoBind

3842

1 L

ZeptoMetrix

0801184

ZeptoBind

839

60 ml

ZeptoMetrix

0801179L

ZeptoCoat

3842

1 L

ZeptoMetrix

0801179

ZeptoCoat

839

60 ml

ZeptoMetrix

0802001

rhIL-2 Recominant Human Interleukin-2

5850

100 ug

ZeptoMetrix

0801060

10x Plate Wash Buffer

293

125 ml

ZeptoMetrix

0801114

rhIL-2 Recominant Human Interleukin-2 (20.000 units)

1463

1.1 ml

ZeptoMetrix

0801017

TCGF (Natural T-Cell Growth Factor, IL-2)

4700

50 ml

ZeptoMetrix

0801195

ZeptoGel

5207

500 ml

ZeptoMetrix

0801002

HIV-1 p24 Antigen ELISA 2.0 Kit

9341

96 det.

ZeptoMetrix

0801221

Horse IgG ELISA

4875

96 det.

ZeptoMetrix

0801008

HIV-1 p24 Antigen ELISA 2.0 Kit

38805

480 det.

ZeptoMetrix

0801437DNA-10UG

Salmonella Enterica Typhimurium Z005, DNA

7001

10 ug

ZeptoMetrix

0801803P

Cryptococcus Neoformans Serotype A, antigen

1092

1.0 ml

ZeptoMetrix

0801202

Rabbit IgG ELISA

6689

96 det.

ZeptoMetrix

0801009

Rat IgG ELISA

4875

96 det.

ZeptoMetrix

0801181

Mouse IgM ELISA

6689

96 det.

ZeptoMetrix

0801201

SIV p27 Antigen ELISA Kit

49764

480 det.

ZeptoMetrix

0801200

HIV-1 p24 Antigen ELISA Kit

38805

480 det.

ZeptoMetrix

0801197

Human IgA ELISA

6689

96 det.

ZeptoMetrix

0801183

Human IgM ELISA

6689

96 det.

ZeptoMetrix

0801182

Human IgG ELISA

6689

96 det.

ZeptoMetrix

0801169

SIV p27 Antigen ELISA Kit

11993

96 det.

ZeptoMetrix

0801137

HIV-1 p24 Extended Range Kit

1346

kit

ZeptoMetrix

0801116

HTLV p19 Antigen ELISA Kit

11993

96 det.

ZeptoMetrix

0801111

HIV-1 p24 Antigen ELISA Kit

9341

96 det.

ZeptoMetrix

0801096

HIV-1 p24 ICx – CRx Kit

1853

100 det.

ZeptoMetrix

0801180

Mouse IgG ELISA

6689

96 det.

ZeptoMetrix

0801693DNA-10UG

Enterococcus Faecalis VRE, DNA

7001

10 ug

ZeptoMetrix

0801084

Anti-HTLV-I gp46 Clone 67 – 5.5.13.1

4388

100 ug

ZeptoMetrix

0801119

Anti-HTLV-II gp46 Clone 73 – 4.9.8

30732

1 mg

ZeptoMetrix

0801120

Anti-HTLV-II p24 Clone 75 – 4.21.11

30732

1 mg

ZeptoMetrix

0801012

Anti-PON1 Clone KRJ1

4388

100 ug

ZeptoMetrix

0801014

Anti-PON1 Clone KRJ2

4388

100 ug

ZeptoMetrix

0801095

Anti-PON1 Clone KRJ1

30732

1 mg

ZeptoMetrix

0801097

Anti-PON1 Clone KRJ2

30732

1 mg

ZeptoMetrix

0801025

Anti-Zika NS1 Clone 1D12

4388

100 ug

ZeptoMetrix

0801032CFHI

HIV Type 1 Strain: IIIB – CFHI

6045

1.0 ml

ZeptoMetrix

0801033CFHI

Human T-Lymphotropic Virus Type I (HTLV-I) – CFHI

6045

1.0 ml

ZeptoMetrix

0801113

Anti-HTLV-II p19 Clone 78 – 6.18.07

30732

1 mg

ZeptoMetrix

0801093

Anti-HTLV-II p19 Clone 78 – 6.18.07

4388

100 ug

ZeptoMetrix

0801094

Anti-HTLV-I gp46 Clone 65 – 6C2.2.34

30732

1 mg

ZeptoMetrix

0801107

Anti-HTLV-I p19 Clone TP-7

30732

1 mg

ZeptoMetrix

0801108

Anti-HTLV-I p19 Clone 45 – 6.11.1.3

30732

1 mg

ZeptoMetrix

0801110

Anti-HTLV-I p24 Clone 46 – 3.24.4

30732

1 mg

ZeptoMetrix

0801127

Anti-HTLV-I gp46 Clone 67 – 5.5.13.1

30732

1 mg

ZeptoMetrix

0801118

Anti-HTLV-I gp46 Clone 68 – 4.11.21

30732

1 mg

ZeptoMetrix

0802004

Anti-HTLV-I gp46 Clone 65 – 6C2.2.34

4388

100 ug

ZeptoMetrix

0801086

Anti-HTLV-II gp46 Clone 73 – 4.9.8

4388

100 ug

ZeptoMetrix

0801087

Anti-HTLV-II p24 Clone 75 – 4.21.11

4388

100 ug

ZeptoMetrix

0801032

Human Immunodeficiency Virus Type 1 (HIV-1) Strain: IIIB Lysate

27593

1 mg

ZeptoMetrix

0801033

Human T-Lymphotropic Virus Type I (HTLV-I) Lysate

31181

1 mg

ZeptoMetrix

0801216

Dinucleotide Standards ThymineGlycolCytidineDimer

1287

50 mg at 1.0 OD

ZeptoMetrix

0801217

Dinucleotide Standards ThymineGlycolAdenineDimer

1287

50 mg at 1.0 OD

ZeptoMetrix

0801218

Nucleoside Standards 8-Oxo-dG

1287

1 mg

ZeptoMetrix

0801219

Nucleoside Standards 8-Oxo-dA

1365

1 mg

ZeptoMetrix

0801384

PON Standards

5265

600 ul

ZeptoMetrix

0801500

Simian Immunodeficiency Virus (SIV) Kit (10 Strip)

9360

10 det.

ZeptoMetrix

0801501

Simian Immunodeficiency Virus (SIV) Kit (30 Strip)

22854

30 det.

ZeptoMetrix

0801502

Simian Immunodeficiency Virus (SIV) Strips (10/tube)

5343

10 strips

ZeptoMetrix

0801021

Zika (PRVABC59) Strips (10/tube)

5343

10 strips

ZeptoMetrix

0801215

Dinucleotide Standards ThymineGlycolThymineDimer

1287

50 mg at 1.0 OD

ZeptoMetrix

0801214

Dinucleotide Standards ThymineGlycolGuanineDimer

1287

50 mg at 1.0 OD

ZeptoMetrix

0801808

Scedosporium prolificans Z083

8775

1.0 ml

ZeptoMetrix

0801192

TBARS Assay kit

7995

160 det.

ZeptoMetrix

0801199

Arylesterase – Paraoxonase Assay Kit

7995

200 tests

ZeptoMetrix

0805002

Total Glutathione Peroxidase Assay Kit

7625

100 tests

ZeptoMetrix

0805004

Glutathione Reductase Assay Kit

10257

100 tests

ZeptoMetrix

0801210

Dinucleotide Standards Formamido Guanine Dimer

1287

50 mg at 1.0 OD

ZeptoMetrix

0801211

Dinucleotide Standards Formamido Cytidine Dimer

1287

50 mg at 1.0 OD

ZeptoMetrix

0801212

Dinucleotide Standards Formamido Thymine Dimer

1287

50 mg at 1.0 OD

ZeptoMetrix

0801213

Dinucleotide Standards Formamido Adenine Dimer

1287

50 mg at 1.0 OD

ZeptoMetrix

0801022

Zika (MR-766) Strips (10/tube)

5343

10 strips

ZeptoMetrix

0801085

Anti-HTLV-I gp46 Clone 68 – 4.11.21

4388

100 ug

ZeptoMetrix

0801698DNA-1UG

Pneumocystis Jiroveci Recombinant W303-Pji, Genomic DNA

7001

10 ug

ZeptoMetrix

0801765DNA-1UG

Scedosporium Apiospermum Z085, DNA

4524

1 ug

ZeptoMetrix

0801770DNA-1UG

Fusarium Oxysporum Z084, DNA

4524

1 ug

ZeptoMetrix

0801802DNA-1UG

Tremella Fuciformis Recombinant, DNA

7001

1 ug

ZeptoMetrix

0801806DNA-1UG

Fusarium solani Z081, DNA

4524

1 ug

ZeptoMetrix

0801808DNA-1UG

Scedosporium Prolificans Z083, DNA

4524

1 ug

ZeptoMetrix

0801827DNA-1UG

Aspergillus Niger Z105, DNA

4524

1 ug

ZeptoMetrix

0801834DNA-1UG

Rhizopus oryzae Z106, DNA

4524

1 ug

ZeptoMetrix

0801899DNA-10UG

Plesiomonas shigelloides Z130, DNA

7001

10 ug

ZeptoMetrix

0801901DNA-10UG

Vibrio Cholerae Z132 (toxigenic)

7001

10 ug

ZeptoMetrix

0801761DNA-1UG

Mucor Indicus Z079, DNA

4524

1 ug

ZeptoMetrix

0801759DNA-1UG

Cunninghamella bertholletiae Z080, DNA

4524

1 ug

ZeptoMetrix

0801700DNA-1UG

Cryptosporidium Parvum-S. cerevisiae Recombinant, DNA

7001

1.0 ml

ZeptoMetrix

0801716DNA-1UG

Aspergillus Fumigatus Z014, DNA

4524

1 ug

ZeptoMetrix

0801723DNA-10UG

Serratia Marcescens Z053, DNA

7001

10 ug

ZeptoMetrix

0801697DNA-1UG

Blastomyces Dermatitidis Recombinant W303-Bde, Genomic DNA

7001

1 ug

ZeptoMetrix

0801736DNA-10UG

Haemophilus ducreyi class I, DNA

7001

10 ug

ZeptoMetrix

0801743DNA-10UG

Bacteroides Thetaiotaomicron Z037 DNA

7001

10 ug

ZeptoMetrix

0801745DNA-10UG

Citrobacter Koseri Z039, DNA

7001

10 ug

ZeptoMetrix

0801747DNA-10UG

Escherichia coli O111:NM, DNA

7001

10 ug

ZeptoMetrix

0801755DNA-1UG

Rhizopus Microsporus Z056, DNA

4524

1 ug

ZeptoMetrix

0801004

Anti-HIV-l p24 Clone 38 – 8.7.47

4388

100 ug

ZeptoMetrix

0801005

Anti-HIV-l p17 Clone 32 – 5.8.42

4388

100 ug

ZeptoMetrix

0801124

Anti-HIV-l p24 Clone 32 – 5.17.76

30732

1 mg

ZeptoMetrix

0801125

Anti-HIV-l p24 Clone 38 – 8.7.47

30732

1 mg

ZeptoMetrix

0801126

Anti-HIV-l p24 Clone 39 – 5.1.23

30732

1 mg

ZeptoMetrix

0801128

Anti-HIV-l RT Clone 39 – 4.12.2

30732

1 mg

ZeptoMetrix

0801136

Anti-HIV-l p24 Clone 39 – 5.4A

30732

1 mg

ZeptoMetrix

0801003

Anti-HTLV-I p19 Clone TP-7

4388

100 ug

ZeptoMetrix

0802014

Anti-HIV-l p24 Clone 39 – 6.14

30732

1 mg

ZeptoMetrix

0801018

Anti-HTLV-I p24 Clone 46 – 3.24.4

4388

100 ug

ZeptoMetrix

0801082

Anti-HTLV-I p19 Clone 45 – 6.11.1.3

4388

100 ug

ZeptoMetrix

0801123

Anti-HIV-l p17 Clone 32 – 5.8.42

30732

1 mg

ZeptoMetrix

0801122

Anti-HIV-l p17 Clone 2D11

30732

1 mg

ZeptoMetrix

0801121

Anti-HIV-l gp41 Clone 10E9

30732

1 mg

ZeptoMetrix

0801006

Anti-HIV-l gp41 Clone 10E9

4388

100 ug

ZeptoMetrix

0801007

Anti-HIV-l RT Clone 39 – 4.12.2

4388

100 ug

ZeptoMetrix

0801076

Anti-HIV-l p17 Clone 2D11

4388

100 ug

ZeptoMetrix

0801077

Anti-HIV-l p17 Clone 32 – 1.24.89

4388

100 ug

ZeptoMetrix

0801078

Anti-HIV-l p24 Clone 32 – 5.17.76

4388

100 ug

ZeptoMetrix

0801079

Anti-HIV-l p24 Clone 39 – 5.1.23

4388

100 ug

ZeptoMetrix

0801080

Anti-HIV-l p24 Clone 39 – 5.4A

4388

100 ug

ZeptoMetrix

0801081

Anti-HIV-l p24 Clone 39 – 6.14

4388

100 ug

ZeptoMetrix

0801115

Anti-HIV-l p17 Clone 32 – 1.24.89

30732

1 mg

ZeptoMetrix

 

 

上海金畔生物科技有限公司是实验试剂一站式采购服务商

1:强大的进口辐射能力,血清、抗体、耗材、大部分限制进口品等。

2:产品种类齐全,经营超过700多个品牌,基本涵盖所有生物实验试剂耗材。

3:提供加急服务,货品一般1-2周到货。

4:富有竞争力的价格优势,绝大部分价格有优势。

5:多年积累良好的信誉,大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校,ROCHE,阿斯利康、国药、fisher等药企。

6:我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等几十家国外公司代理。

7:我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等*批发,欢迎合作。