PrimeScript™ 1st Strand cDNA Synthesis Kit酶试剂盒Takara


PrimeScript 1st Strand cDNA Synthesis Kit
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Takara 6110A PrimeScript 1st Strand cDNA Synthesis Kit 50 Rxns ¥1,261 PrimeScript™ 1st Strand cDNA Synthesis Kit PrimeScript™ 1st Strand cDNA Synthesis Kit PrimeScript™ 1st Strand cDNA Synthesis Kit
Takara 6110B (A × 4) PrimeScript 1st Strand cDNA Synthesis Kit 50 Rxns x 4 ¥4,546 PrimeScript™ 1st Strand cDNA Synthesis Kit PrimeScript™ 1st Strand cDNA Synthesis Kit PrimeScript™ 1st Strand cDNA Synthesis Kit
收藏产品 加入购物车

无标题文档

 
 
PrimeScript™ 1st Strand cDNA Synthesis Kit PrimeScript™ 1st Strand cDNA Synthesis Kit PrimeScript™ 1st Strand cDNA Synthesis Kit PrimeScript™ 1st Strand cDNA Synthesis Kit PrimeScript™ 1st Strand cDNA Synthesis Kit
 
PrimeScript™ 1st Strand cDNA Synthesis Kit
PrimeScript™ 1st Strand cDNA Synthesis Kit  
PrimeScript™ 1st Strand cDNA Synthesis Kit
 
 
■ 制品内容 (50 次量)
PrimeScript RTase (200 U/ μl) 50 μl
5X PrimeScript Buffer 200 μl
RNase Inhibitor (40 U/μl) 25 μl
dNTP Mixture (10 mM each) 50 μl
Oligo dT Primer (50 μM) 50 μl
Random 6 mers (50 μM) 100 μl
RNase free dH2O 1 ml
 
■ 制品说明
本制品是使用PrimeScript RTase从Total RNA或PolyA+RNA合成1st Strand cDNA的试剂盒,含有1st Strand cDNA合成所需的全部试剂。 PrimeScript Reverse Transcriptase是一种MMLV (Moloney Murine Leukemia virus) 由来的新型反转录酶。本酶能够有效合成长达12 kb的1st Strand cDNA,即使对富含GC的片段或者具有复杂二级结构的RNA,在常规的反转录温度42℃条件下也能得到良好的反转录效果,无需进行高温反转录反应 (高温反转录反应会导致RNA的降解)。合成的1st Strand cDNA 可广泛应用于2nd Strand合成、杂交、PCR扩增、Real Time PCR反应等。
 
■ 各种引物的序列
引物名称 引物序列
       Random 6 mers        pd(N)6
       Oligo dT Primer        Takara特别开发的dT区域的序列*
* 该序列和TaKaRa RNA PCR Kit (AMV) Ver.3.0 (Code No. RR019A/B) 的Oligo dT Adaptor Primer不同,不含有M13 Primer M4的序列。
 
■ 保存
-20℃。