Human DNA (cytosine-5) Methyltransferase (Dnmt1) | NEB酶试剂 New England Biolabs

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Human DNA (cytosine-5) Methyltransferase (Dnmt1)

  • Catalog # M0230 was discontinued on December 15, 2021
Human DNA (cytosine-5) Methyltransferase (Dnmt1) | NEB酶试剂 New England Biolabs

Dnmt1 methylates cytosine residues in hemimethylated DNA in the sequence of 5’…CG…3′ (1,2). Mammalian Dnmt1 is believed to be involved in carcinogenesis, embryonic development and several other biological functions (3-5).

  • Supplied with 10x DNMT1 Buffer, 100X BSA and 32 mM S-adenosylmethionine (SAM)

Human DNA (cytosine-5) Methyltransferase (Dnmt1) | NEB酶试剂 New England Biolabs

产品信息

The bulk of the methylation takes place during DNA replication in the S-phase of the cell cycle (6).

产品来源

Dnmt1 is expressed from human Dnmt1 cDNA using a baculovirus expression system (1,7).

产品类别:
Discontinued Products

  • 特性和用法

    单位定义

    One unit is the amount of enzyme required to catalyze the transfer of 1 pmol of methyl group to poly dI.dC substrate in a total reaction volume of 25 μl in 30 minutes at 37°C.

    反应条件

    1X Dnmt1 Reaction Buffer
    Supplement with 100 µg/ml BSA, Molecular Biology Grade 和 160 µM S-adenosylmethionine (SAM)
    Incubate at 37°C

    使用浓度

    2,000 units/ml

    贮存溶液

    50 mM Tris-HCl
    200 mM NaCl
    1 mM DTT
    1 mM EDTA
    50% Glycerol
    pH 7.5 @ 25°C

    热失活

    65°C for 20 min

  • 相关产品

    单独销售的组分

    • Purified BSA
    • S-腺苷甲硫氨酸（SAM）

  • 注意事项
    1. For DNA modification and protection applications, M.SssI (NEB #M0226) is preferred because it efficiently methylates both unmethylated and hemimethylated DNA substrates.

  • 参考文献
    1. Pradhan, S. et al. (1999). J. Biol. Chem. . 274, 33002-33010.
    2. Bacolla, A. et al. (1999). J. Biol. Chem. . 274, 33011-33019.
    3. Schmutte, C. et al. (1998). Biol. Chem. . 379, 377-388.
    4. Laird, P.W. et al. (1995). Cell. 81, 197-205.
    5. Li, E. et al. (1992). Cell. 12, 915-926.
    6. Leonhardt, H. et al. (1992). Cell. 71, 865-873.
    7. Yen, R.W. et al. (1992). Nucl. Acids. Res. . 20, 2287-2291.

FAQs & 问题解决指南

  • FAQs
    1. Is S-adenosylmethionine (SAM) supplied with the Methyltransferase?
    2. What source of tritiated SAM is recommended for use with Dnmt1?
    3. What should be considered if the methylation using Dnmt1 is not going to completion?
    4. How does Dnmt1 differ from SssI methylase?
    5. Can Dnmt1 be heat inactivated?
    6. Will any NEB methyltransferases (methylases) work on RNA?

引用 & 技术文献

  • 引用文献

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    • M0230S_L_v1

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    • M0230S_v1_10009673
    • M0230L_v1_10028052
    • M0230S_v1_10028046
    • M0230L_v1_10035552
    • M0230S_v1_10033941
    • M0230L_v1_10042377
    • M0230S_v1_10042375
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    • M0230S_v1_10059149
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    • M0230L_v1_10086920
    • M0230S_v1_10094047
    • M0230L_v1_10100068
    • M0230S_v1_10100065
    • M0230L_v1_10117330

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    • Human DNA (cytosine-5) Methyltransferase (Dnmt1)