HeLa Genomic DNA |NEB酶试剂 New England Biolabs

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HeLa Genomic DNA

  • Catalog # N4006 was discontinued on December 15, 2021
  • Highly pure HeLa Genomic DNA for use in a wide variety of applications including: PCR, SNP analysis and southern blotting
  • Genomic DNA library construction
  • Control DNA for Methylation-specific PCR (MSP), Bisulfite sequencing, Methylation-sensitive Single-Nucleotide Primer Extension (MS-SNuPE,) Combined Bisulfite Restriction Analysis (COBRA), Bisulfite treatment and PCR-single strand Confirmation Polymorphism Analysis (Bisulfite-PCR-SSCP/BiPS)

HeLa Genomic DNA |

产品信息

Human female HeLa genomic DNA.

Product Source:
 
HeLa (cervix adenocarcinoma) cells were grown to confluency in DMEM plus 10% fetal bovine serum. Genomic DNA was isolated by a standard genomic purification protocol (1), phenol extracted and equilibrated to 10 mM TrisHCl (pH 7.5) and 1 mM EDTA.

产品类别:
Discontinued Products

  • 特性和用法

    贮存溶液

    10 mM Tris-HCl
    1 mM EDTA
    pH 7.5 @ 25°C

  • 优势和特性

    应用特性

    • A control for Methylation-Specific PCR (MSP) (2), Bisulfite sequencing, Methylation-sensitive Single-Nucleotide Primer Extension (Ms-SNuPE), Combined Bisulfite Restriciton Analysis (COBRA), Bisulfite treatment and PCR-Single-Strand Conformation Polymorphism Analysis (Bisulfite-PCR-SSCP/BiPS).
    • PCR, SNP Analysis, Southern Blotting
    • Genomic DNA library construction

  • 注意事项
    1. Avoid multiple freeze/thaw cycles.

  • 参考文献
    1. Sambrook, J. and Russell, D. (2001). Molecular Cloning: A Laboratory Manual. (3rd ed.), (pp. 6.4-6.12). Cold Spring Harbor: Cold Spring Ha.

引用 & 技术文献

  • 引用文献

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质控、安全 & 法规

  • 质控分析
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  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • N4006S_v1

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • N4006S_v1_0051603
    • N4006S_v1_0051608
    • N4006S_v1_0051612
    • N4006S_v1_0051704
    • N4006S_v1_0051708
    • N4006S_v1_0051711
    • N4006S_v1_10009459
    • N4006S_v1_10026020
    • N4006S_v1_10036630
    • N4006S_v1_10058101
    • N4006S_v1_10073960
    • N4006S_v1_10077859
    • N4006S_v1_10097031
    • N4006S_v1_10116296

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • HeLa Genomic DNA