Taq PCR Kit | NEB酶试剂 New England Biolabs

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Taq PCR Kit

The industry standard

  • Ideal for routine PCR applications
  • Exceptional value in terms of cost per unit
  • Kit format includes optimized reagents and enables flexible experimental design for 200 PCR reactions

Taq PCR Kit | NEB酶试剂 New England Biolabs

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
E5000S 不适用 200 reactions ¥1,249.00 有 无 有 无 无
如何订购
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产品信息

Taq PCR Kit | NEB酶试剂 New England Biolabs

The Taq PCR Kit contains a sufficient supply of recombinant, highly purified Taq DNA Polymerase, PCR-qualified buffer solutions, deoxynucleotides and a broad-range, pre-mixed, ready-to-load DNA marker to perform 200 PCR reactions.

重点

Robust and reliable reactions
Tolerates a wide range of templates
Incorporates dUTP, dITP and fluorescently-labeled nucleotides

产品类别:
Taq DNA Polymerase Products

应用:
Multiplex PCR,
Specialty PCR,
Routine PCR,

PCR

  • 试剂盒组成

    Kit 组分

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • E5000S     -20    
        Deoxynucleotide (dNTP) Solution Mix N0447SVIAL -20 1 x 0.2 ml 10 mM each dNTP
        Quick-Load® Purple 1 kb Plus DNA Ladder N0550AVIAL 4 1 x 0.2 ml 100 µg/ml
        Standard Taq Reaction Buffer Pack B9014SVIAL -20 1 x 1.5 ml 10 X
        Standard Taq (Mg-free) Reaction Buffer Pack B9015SVIAL -20 1 x 1.5 ml 10 X
        Magnesium Chloride (MgCl2) Solution B9021SVIAL -20 1 x 1.5 ml 25 mM
        Taq DNA Polymerase with Standard Taq Buffer M0273AVIAL -20 1 x 0.05 ml 5,000 units/ml

  • 特性和用法

  • 相关产品

    相关产品

    • Taq DNA Polymerase with ThermoPol® Buffer
    • Deoxynucleotide (dNTP) Solution Set
    • ThermoPol® Reaction Buffer Pack
    • OneTaq® Hot Start DNA Polymerase
    • Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

    单独销售的组分

    • Taq DNA Polymerase with Standard Taq Buffer
    • Standard Taq Reaction Buffer Pack
    • Standard Taq (Mg-free) Reaction Buffer Pack
    • Magnesium Chloride (MgCl2) Solution
    • Deoxynucleotide (dNTP) Solution Mix
    • Quick-Load® Purple 1 Kb Plus DNA Ladder

  • 参考文献
    1. Saiki, R.K. et al. (1985). Science. 230, 1350-1354.
    2. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
    3. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
    4. Lawyer, F.C. et al. (1993). PCRMethod and Appl.. 2, 275-287.
    5. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res.. 18, 7317-7322.
    6. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
    7. Powell, L.M. et al. (1987). Cell. 50, 831-840.

操作说明、说明书 & 用法

  • 操作说明
    1. Protocol for a Routine Taq PCR
    2. Taq DNA Polymerase Guidelines for PCR Optimization

  • 说明书
    产品说明书包含产品使用的详细信息、产品配方和质控分析。

    • ManualE5000

  • 使用指南
    • Activity of Restriction Enzymes in PCR Buffers
    • Guidelines for PCR Optimization with Taq DNA Polymerase
    • Guidelines for PCR Optimization with Thermophilic DNA Polymerases

工具 & 资源

  • 选择指南
    • DNA Polymerase Selection Chart
    • Thermophilic DNA Polymerases

  • Web 工具
    • Tm Calculator

FAQs & 问题解决指南

  • 问题解决指南
    • PCR Troubleshooting Guide
    • Taq PCR Kit Troubleshooting Guide

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    Taq PCR Kit | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • E5000S_v2

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • E5000S_v1_0561712
    • E5000S_v2_10013531
    • E5000S_v2_10015410
    • E5000S_v2_10024366
    • E5000S_v2_10035559
    • E5000S_v2_10043668
    • E5000S_v2_10046994
    • E5000S_v2_10048559
    • E5000S_v2_10054337
    • E5000S_v2_10055106
    • E5000S_v2_10057980
    • E5000S_v2_10062513
    • E5000S_v2_10068733
    • E5000S_v2_10072101
    • E5000S_v2_10077362
    • E5000S_v2_10086527
    • E5000S_v2_10092410
    • E5000S_v2_10098681
    • E5000S_v2_10107012
    • E5000S_v2_10117811
    • E5000S_v2_10122374
    • E5000S_v2_10127965
    • E5000S_v2_10139587
    • E5000S_v2_10145226
    • E5000S_v2_10147604
    • E5000S_v2_10161055

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • Deoxynucleotide (dNTP) Solution Mix
    • Quick-Load® Purple 1 kb Plus DNA Ladder
    • Standard Taq Reaction Buffer Pack
    • Standard Taq (Mg-free) Reaction Buffer Pack
    • Magnesium Chloride (MgCl2) Solution
    • Taq DNA Polymerase with Standard Taq Buffer