Hot Start Taq 2X Master Mix | NEB酶试剂 New England Biolabs

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Hot Start Taq 2X Master Mix

The industry standard with the hot start peformance

  • Ideal for routine PCR applications
  • Aptamer-based hot start technology avoids nonspecific amplification and enables room temperature reaction setup
  • 2X master mix format for easy reaction setup – just add template DNA and primers

Hot Start Taq 2X Master Mix | NEB酶试剂 New England Biolabs

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0496S 2 X 100 reactions (50 µl) ¥699.00 有 无 无 有 无
M0496L 2 X 500 reactions (50 µl) ¥2,779.00 无 无 无 无 无
如何订购
Please enter a quantity for at least one size

产品信息

Hot Start Taq 2X Master Mix | NEB酶试剂 New England Biolabs  
Hot Start Taq 2X Master Mix is an optimized ready-to-use solution containing Hot Start Taq DNA Polymerase, dNTPs, MgCl2, KCl and stabilizers. It is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. It is recommended for amplification up to 5 kb.

Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions allowing reactions to be set up at room temperature. The aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. Hot Start Taq DNA Polymerase possesses a 5´→ 3´ polymerase activity1,2,3 and a 5´ flap endonuclease activity.4,5

产品来源

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1

产品类别:
Taq DNA Polymerase Products,
Master Mixes Products

应用:
Multiplex PCR,
Specialty PCR,
Routine PCR,

PCR

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0496S     -20    
        Hot Start Taq 2X Master Mix M0496SVIAL -20 2 x 1.25 ml 2 X
    • M0496L     -20    
        Hot Start Taq 2X Master Mix M0496SVIAL -20 10 x 1.25 ml 2 X

  • 特性和用法

    1X 预混液组分

    10 mM Tris-HCl
    50 mM KCl
    1.5 mM MgCl2
    0.2 mM dNTPs
    5% Glycerol
    25 units/ml Hot Start Taq DNA polymerase
    pH 8.3 @ 25°C

    热失活

    否

    单位活性检测条件

    1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.

  • 优势和特性

    应用特性

    • High-specificity PCR
    • Routine PCR
    • High-throughput PCR
    • Microarray Analysis
    • Colony PCR

  • 相关产品

    相关产品

    • Deoxynucleotide (dNTP) Solution Set
    • Deoxynucleotide (dNTP) Solution Mix
    • Hot Start Taq DNA Polymerase
    • Magnesium Chloride (MgCl2) Solution
    • Standard Taq Reaction Buffer Pack

  • 注意事项
    1. Hot Start Taq 2X Master Mix is stable for fifteen freeze-thaw cycles when stored at -20°C.
    2. Hot Start Taq 2X Master Mix is also stable for three months at 4°C, so for frequent use, an aliquot may be kept at 4°C.

  • 参考文献
    1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, PubMedID: 1550-1557
    2. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
    3. Lawyer, F.C. et al. (1993). PCR Methods and Appl.. 2, 275-287.
    4. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res.. 18, 7317-7322.
    5. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.

操作说明、说明书 & 用法

  • 操作说明
    1. Protocol for a PCR reaction using Hot Start Taq 2X Master Mix (M0496)

  • 使用指南
    • Activity of Restriction Enzymes in PCR Buffers

工具 & 资源

  • Web 工具
    • Tm Calculator

FAQs & 问题解决指南

  • FAQs
    1. Does Hot Start Taq 2X Master Mix require an extended initial incubation to activate the polymerase?
    2. How should I set up a PCR using the Hot Start Taq 2X Master Mix?
    3. What type of DNA ends result from a primer extension reaction or a PCR reaction using Hot Start Taq DNA Polymerase?
    4. When should Hot Start Taq DNA Polymerase be used in a primer extension reaction or for PCR?
    5. Will the 5’→3′ flap endonuclease activity of Hot Start Taq DNA Polymerase degrade primers?
    6. Can Hot Start Taq DNA Polymerase be used for nick translation?
    7. How should I determine an appropriate annealing temperature for my reaction?
    8. What is the longest amplicon that can be obtained with Hot Start Taq DNA Polymerase?
    9. Can Hot Start Taq DNA Polymerase be used with uracil-containing primers or bisulfite-treated DNA?
    10. Where can I find help troubleshooting my PCR?

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    Hot Start Taq 2X Master Mix | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

  • 专题文章
    • Using aptamers to control enzyme activities: Hot Start Taq and beyond

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • M0496S_L_v1
    • M0496S_L_v2

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • M0496S_L_v1_0141708
    • M0496S_L_v1_0151711
    • M0496L_v1_10010142
    • M0496L_v1_10015782
    • M0496S_v1_10016612
    • M0496L_v1_10018581
    • M0496S_v1_10034409
    • M0496S_v1_10036842
    • M0496L_v1_10036841
    • M0496S_v1_10042501
    • M0496L_v1_10047069
    • M0496S_v1_10047072
    • M0496S_v1_10048092
    • M0496S_v1_10049901
    • M0496L_v1_10049903
    • M0496L_v1_10053496
    • M0496S_v1_10051675
    • M0496S_v1_10055502
    • M0496L_v1_10055504
    • M0496S_v1_10057984
    • M0496L_v1_10063389
    • M0496S_v2_10109357
    • M0496L_v2_10116752
    • M0496L_v2_10122337
    • M0496S_v2_10123074
    • M0496L_v2_10128593
    • M0496L_v2_10131796
    • M0496S_v2_10130703
    • M0496L_v2_10140723
    • M0496S_v2_10141017
    • M0496L_v2_10146009
    • M0496L_v2_10148885
    • M0496L_v2_10151434
    • M0496S_v2_10153270
    • M0496L_v2_10159482
    • M0496S_v2_10161970
    • M0496L_v2_10161971

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • Hot Start Taq 2X Master Mix