OneTaq® Hot Start 2X Master Mix with GC Buffer | NEB酶试剂 New England Biolabs

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OneTaq® Hot Start 2X Master Mix with GC Buffer

The ONE polymerase for your endpoint PCR needs

OneTaq® DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases for use with routine and difficult PCR experiments.

  • Unique hot start formulation eliminates nonspecific amplification and does not require a separate activation step
  • Obtain high yields with GC rich templates (>65%)
  • Master mix is a 2X concentrated solution containing everything needed for robust amplification

精选视频

OneTaq® Hot Start 2X Master Mix with GC Buffer | NEB酶试剂 New England Biolabs

How to Amplify GC-rich DNA

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库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0485S 2 X 100 reactions (50 µl) ¥719.00 有 无 无 无 有
M0485L 2 X 500 reactions (50 µl) ¥3,249.00 无 无 无 无 无
如何订购
Please enter a quantity for at least one size

产品信息

OneTaq® Hot Start 2X Master Mix with GC Buffer is an optimized blend of Taq and Deep Vent® DNA Polymerases combined with an aptamer-based inhibitor. This enzyme blend is ideally suited to PCR applications from GC-rich templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient master mix formulation contains dNTPs, MgSO4, buffer components and stabilizers, requiring only the addition of primers and DNA template for robust amplification.

Comparison of OneTaq Products to Other Commercially Available Polymerases

OneTaq® Hot Start 2X Master Mix with GC Buffer | NEB酶试剂 New England Biolabs 

Amplification of a selection of high GC human genomic DNA targets demonstrates OneTaq performance. All polymerases were cycled according to manufacturer’s recommendations, including the use of additives to enhance the amplification of targets with high GC content. Yield (dot size) and purity (color) of reaction product were quantified from triplicate reactions on a Perkin Elmer LabChip®. A large, dark green dot represents the highest yield and purity.

OneTaq® Hot Start 2X Master Mix with GC Buffer | NEB酶试剂 New England Biolabs

产品来源

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent® DNA Polymerase gene from Pyrococcus species GB-D.

产品类别:
OneTaq® DNA Polymerases Products,
Taq DNA Polymerase Products,
Master Mixes Products

应用:
Hot Start PCR,
Multiplex PCR,
Specialty PCR,

Routine PCR,

PCR

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0485S     -20    
        OneTaq® Hot Start 2X Master Mix with GC Buffer M0485SVIAL -20 2 x 1.25 ml 2 X
        OneTaq® High GC Enhancer B9026AVIAL -20 1 x 2 ml 不适用
    • M0485L     -20    
        OneTaq® Hot Start 2X Master Mix with GC Buffer M0485SVIAL -20 10 x 1.25 ml 2 X
        OneTaq® High GC Enhancer B9026AVIAL -20 3 x 2 ml 不适用

  • 特性和用法

    1X 预混液组分

    80 mM Tris-SO4
    20 mM (NH4)2SO4
    2 mM MgSO4
    5% Glycerol
    5% DMSO
    0.06% IGEPAL® CA-630
    0.05% Tween® 20
    0.2 mM dNTPs
    25 units/ml OneTaq Hot Start DNA Polymerase
    pH 9.2 @ 25°C

    热失活

    否

    单位活性检测条件

    1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.

  • 优势和特性

    Features

    • GC-rich PCR
    • High Sensitivity PCR
    • High Throughput PCR
    • Colony PCR
    • Long PCR (up to ~6 kb genomic)

  • 相关产品

    相关产品

    • Magnesium Sulfate (MgSO4) Solution
    • OneTaq® DNA Polymerase
    • OneTaq® Hot Start DNA Polymerase
    • OneTaq® Hot Start 2X Master Mix with Standard Buffer
    • OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer
    • OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

  • 注意事项
    1. OneTaq Hot Start 2X Master Mix with GC Buffer is stable for fifteen freeze-thaw cycles when stored at -20°C.
    2. OneTaq Hot Start 2X Master Mix with GC Buffer is also stable for one month at 4°C, so for frequent use, an aliquot may be kept at 4°C.
    3. Product specifications for individual components in the OneTaq Hot Start 2X Master Mix with GC Buffer are available separately.

  • 参考文献
    1. Barnes,W.M. (1994). Proc. Natl. Acad. Sci. USA. 91, 2216-2220.
    2. Saiki, R.K. et al. (1985). Science. 230, 1350-1354.
    3. Powell, L.M. et al. (1987). Cell. 50, 831-840.

操作说明、说明书 & 用法

  • 操作说明
    1. Protocol for OneTaq Hot Start 2X Master Mix with GC Buffer (M0485)

  • 使用指南
    • Activity of Restriction Enzymes in PCR Buffers
    • Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases
    • Guidelines for PCR Optimization with Thermophilic DNA Polymerases

  • 应用实例
    • Robust Colony PCR from Multiple E. coli Strains using OneTaq® Quick-Load® Master Mixes

工具 & 资源

  • 选择指南
    • DNA Polymerase Selection Chart
    • Thermophilic DNA Polymerases

  • Web 工具
    • Tm Calculator

FAQs & 问题解决指南

  • FAQs
    1. How should I set up a PCR using the OneTaq® Hot Start Master Mixes?
    2. Can I use my regular Taq-based cycling conditions for OneTaq® Hot Start DNA Polymerase based products?
    3. How do I activate OneTaq® Hot Start Polymerase?
    4. What are the stability and storage requirements of the Quick-Load® OneTaq® Master Mixes?
    5. What type of DNA ends result from a primer extension reaction or a PCR using OneTaq® DNA Polymerase?
    6. How long a product can be made by OneTaq® DNA Polymerase?
    7. Can OneTaq® DNA Polymerase be used with uracil-containing primers or bisulfite-treated DNA?
    8. What is the fidelity of OneTaq® DNA Polymerase?
    9. When should I add the High GC Enhancer?
    10. Where can I find help troubleshooting my PCR?
    11. How should I determine an appropriate annealing temperature for my reaction?
    12. How is OneTaq DNA Polymerase different from LongAmp™ Taq DNA Polymerase?

  • 问题解决指南
    • PCR Troubleshooting Guide

  • 实验技巧
    Try using a master mix with OneTaq Hot Start DNA Polymerase to achieve highly specific and sensitive PCR amplification.

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    OneTaq® Hot Start 2X Master Mix with GC Buffer | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

  • 专题文章
    • Using aptamers to control enzyme activities: Hot Start Taq and beyond

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • M0485S_L_v1
    • M0485S_L_v2

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • M0485S_L_v1_0191703
    • M0485S_L_v1_0201708
    • M0485S_L_v1_0211711
    • M0485L_v1_10013984
    • M0485L_v1_10014391
    • M0485L_v1_10014779
    • M0485S_v1_10014778
    • M0485L_v1_10016113
    • M0485S_v1_10021835
    • M0485L_v1_10021836
    • M0485S_v1_10034531
    • M0485L_v1_10034532
    • M0485L_v1_10037121
    • M0485S_v1_10038572
    • M0485L_v1_10046053
    • M0485L_v1_10048408
    • M0485S_v1_10048405
    • M0485L_v1_10048758
    • M0485L_v1_10051698
    • M0485S_v1_10054118
    • M0485S_v1_10055500
    • M0485L_v1_10056297
    • M0485L_v1_10061498
    • M0485S_v1_10061497
    • M0485L_v1_10063559
    • M0485S_v2_10067250
    • M0485L_v2_10068724
    • M0485S_v2_10092093
    • M0485S_v2_10096454
    • M0485S_v2_10098708
    • M0485L_v2_10092095
    • M0485L_v2_10106321
    • M0485L_v2_10111730
    • M0485S_v2_10111731
    • M0485S_v2_10120969
    • M0485L_v2_10126937
    • M0485S_v2_10127987
    • M0485L_v2_10137290
    • M0485L_v2_10150851
    • M0485S_v2_10152167
    • M0485L_v2_10155210
    • M0485S_v2_10158235
    • M0485L_v2_10158234
    • M0485S_v2_10162336
    • M0485L_v2_10162335

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • OneTaq® Hot Start 2X Master Mix with GC Buffer
    • OneTaq® High GC Enhancer