NEBNext^® ARTIC SARS-CoV-2 Library Prep Kit (Illumina^®)

The NEBNext^® ARTIC kits were developed in response to the critical need for reliable and accurate methods for SARS-CoV-2 sequencing. These kits, for long and short read sequencing, were based on the original work of the ARTIC Network (1). The ARTIC SARS-CoV-2 sequencing workflow is a multiplexed amplicon-based whole-viral-genome sequencing approach. 

NEBNext ARTIC kits include primers and reagents for RT-PCR from SARS-CoV-2 gRNA and downstream library preparation for Illumina® and Oxford Nanopore Technologies^® sequencing. The V3 ARTIC primers have been balanced, using methodology developed at NEB based on empirical data from sequencing. In combination with optimized reagents for RT-PCR, the kits deliver improved uniformity of amplicon yields from gRNA across a wide copy number range.  

If shorter inserts are preferred, the NEBNext ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina) (NEB #E7658) generates libraries with ~150 bp inserts, compatible with 2 x 75 bp sequencing.

• Information on primer variant overlaps can be found here
• Streamlined, high-efficiency protocol
• Effective with a wide range of viral genome inputs (10-10,000 copies)
• Improved uniformity of SARS-CoV-2 genome coverage depth with a more balanced primer pool
• No requirement for amplicon normalization prior to library preparation
• Library inserts in the 400 bp range
• Compatible with 2 x 250 Illumina sequencing
• Includes NEBNext Sample Purification Beads (SPRIselect®)
• Adaptors and primers available separately; For a 384 unique dual index format, please contact us
• For a bulk format of the kit (2,304 reactions) please contact us

1. (Josh Quick 2020. nCoV-2019 sequencing protocol v2 (GunIt). protocols.io https://dx.doi.org/10.17504/protocols.io.bdp7i5rn).