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Yeast Carbon Base Medium Powder
This medium pack includes 12 grams of Yeast Carbon Base Medium Powder and 10 ml of Acetamide Solution.
The Yeast Carbon Base Medium Powder contains sufficient reagents needed to make 1 liter of Yeast Carbon Base (YCB) Agar Medium containing 5 mM acetamide.
YCB Agar Medium is used for acetamide selection of K. lactis cells that have been transformed with a pKLAC1-based expression vector (NEB #N3740). The medium contains glucose and all nutrients needed to sustain growth of K. lactis GG799 cells except a simple nitrogen source. Only transformed cells can utilize acetamide provided in the medium as a source of nitrogen after it is broken down to ammonia by acetamidase (the product of the amdS gene present in pKLAC1).
