cell biolabs VPK-156说明书

QuickTiter™ MuLV Core Antigen ELISA Kit

产品描述：

将一种抗mulv p30单克隆包衣抗体吸附在微量滴定板上。样品或标准品中存在的mulv核心抗原（p30）与吸附在板上的抗体结合；添加抗mulv p30多克隆抗体并与第一抗体捕获的抗原结合。在孵育和洗涤步骤后，添加一种HRP结合的二级抗体并结合到抗mulv p30多克隆。在洗涤过程中去除未结合的HRP结合的二级抗体，并将与HRP反应的底物溶液加入到井中。有色产物的形成与样品中存在的mulv核心抗原的量成比例。通过添加酸终止反应，并在450 nm处测量吸光度。用重组mulv p30核心抗原制备标准曲线，测定样品浓度。

检测方法：Colorimetric

检测样本：MuLV samples

试剂盒组成：

Box 1 (shipped at room temperature)

Anti-MuLV p30 Antibody Coated Plate                  96wells

Anti-MuLV p30 Polyclonal Antibody                    20 ul

Secondary Antibody, HRP Conjugate                    20 ul

Assay Diluent 50 ml

Triton X-100 Solution                       50 ml

10X Wash Buffer                                    100 ml

Substrate Solution                                    12 ml

Stop Solution                                        12ml

Substrate Solution                                    12ml

Stop Solution                                        12ml

Box 2 (shipped on blue ice packs)

Recombinant MuLV p30 Standard                        100 ul

保存条件：Upon receipt, aliquot and store the Recombinant MuLV p30 Standard at -20ºC to avoid multiple freeze/thaw cycles.  Store all other kit components at 4ºC.

 

标准曲线图：

MuLV Core Antigen ELISA Standard Curve.

Purification of Recombinant MuLV p30 Protein. Lane 1: MW STDs; Lane 2: E.Coli Whole Lysate (-IPTG); Lane 3: E.Coli Whole Lysate (+IPTG); Lane 4: Crude Lysate; Lane 5: Lysate after Ni-NTA beads; Lane 6: Bead Wash; Lane 7: Elution Fraction for Recombinant MuLV p30 Standard.