mrcppureagents cDNA克隆产品


 

mrcppureagents始于1998年,致力于协助MRC蛋白磷酸化和泛素化单位(MRC PPU)的一部分的科学家。我们自己的团队由具有分子生物学和生物化学背景的个人组成,他们经过培训可提供符合MRC预期标准的试剂PPU。

在科学中共享试剂是​​*的,并可以促进协作,但也可以促进发现并验证过去的结果。这些步骤有助于推动科学向前发展。MRC PPU的研究人员已经发表了前沿科学多年,并且正如预期的那样,广泛要求他们论文中使用的工具来促进其他人的工作以及整个领域。

考虑到所需试剂的数量,我们在2013年创建了一个网站,以方便访问数据库中的这些研究工具。尽管我们初的投资组合开始专注于遍在蛋白系统中的激酶,磷酸酶和酶,但我们的科学家-因此我们的试剂-已扩展到新领域。我们欢迎您查看我们不断扩大的试剂目录,以找出可能有助于您自己的研究工作的试剂。

此外,我们很乐意为研究人员提供定制生成的蛋白质或抗体的帮助。迄今为止,我们已经与来自学术界以及制药和生物技术领域的世界各地的研究人员合作完成了许多项目。请让我们知道我们将如何帮助您!

mrcppureagents cDNA克隆产品| MRC PPU试剂和服务

感兴趣的cDNA的克隆和亚克隆是分子生物学的基础,并有助于随后的生化和细胞生物学研究。尽管看似常规,但我们创建和验证克隆的方法仍保持一定程度的严格性,以确保所有克隆均符合本单位科学家所要求的准确度标准。

通过测序验证所有cDNA插入片段,然后将原始测序迹线与参考序列(Genbank)进行比对以进行验证。我们的工厂利用DNA Dynamo进行此比对。SNAPGENE,一个免费的软件程序可以打开这些文件进行查看。请参考我们的参考资料部分,以识别其他资源,这些资源可以帮助您检查和操作所需的测序文件。提供了每种构建体的详细数据表,其中列出了质粒的详细信息以及的核苷酸和蛋白质序列。

请注意:我们的投资组合中有一部分构造可能没有完整的可用数据集。这些是较旧的克隆,可在设备中使用,而当时并不一定要存储序列数据。对于要求使用这些较早构建体的任何研究人员,我们将自费对它们进行重新排序,并在发出该构建体之前生成数据表。   

 

所有cDNA克隆都以10ul的体积提供,浓度在10-100 ng /微升之间(取决于拷贝数)-在您自己的实验室中有足够的数量用于转化和繁殖每个构建体!

 

Type Gene Name Plasmid Genbank Species Tag Vector Type Price per aliquot
cDNA Clones MRCKView Data pcDNA FRT/TO HA MRCK beta 1-473 D218A NM_006035.3   HA Mammalian £110.00
cDNA Clones UVRAGView Data pcDNA5D FRT/TO 3xflag UVRAG S508A   Human FLAG Mammalian £110.00
cDNA Clones UVRAGView Data pcDNA5D FRT/TO 3xFlag UVRAG S508A S509A   Human FLAG Mammalian £110.00
cDNA Clones (chicken) CDKL5View Data pcDNA5D FRT/TO Chicken CDKL5 TEV GST 6His Sequence provided by Michael Morgan Chicken GST Mammalian £110.00
cDNA Clones (chicken) CDKL5View Data pEX Chicken CDKL5 TEV GST 6His Sequence provided by Michael Morgan. Chicken GST, 6His Bacterial £110.00
cDNA Clones (TEV)-Ubiquitin-UbiquitinView Data pGST-1-(blunt)TEV_diubiquitin P62987 Human GST Bacterial £110.00
cDNA Clones (TEV)-Ubiquitin-UbiquitinView Data pSc-B-(blunt)TEV_diubiquitin P62987     Other £110.00
cDNA Clones -View Data pSuperior Neo GFP- shRNA-NegativeControl   GFP Mammalian £110.00
cDNA Clones 132View Data pGL4.13 132 comp (1 copy)       Mammalian £110.00
cDNA Clones 13376836View Data pCMV-HA 13376836 NM_025232 Human HA Mammalian £110.00
cDNA Clones 13376836View Data pGEX 1337836 NM_025232 Human GST Bacterial £110.00
cDNA Clones 13376836View Data TOPO-13376836       Other £110.00
cDNA Clones 13376836 (presenilin like)View Data pGFP-HA 13376836 NM_025232 Human GFP, HA Mammalian £110.00
cDNA Clones 14-3-3 betaView Data pcDNA5-FRT/TO-mCherry 14-3-3 beta XM_017028039.1 Human mCherry Mammalian £110.00
cDNA Clones 14-3-3 betaView Data pEBG6P-1 14-3-3 beta NM_139323.2 Human GST Mammalian £110.00
cDNA Clones 14-3-3 betaView Data pET28a 14-3-3 beta NP_647539 Human 6His Bacterial £110.00
cDNA Clones 14-3-3 betaView Data pGEX6P-14-3-3 beta NM_139323.2 Human GST Bacterial £110.00
cDNA Clones 14-3-3 beta (mouse)View Data pBluescript KS+mouse 14-3-3 beta NM_018753.6 GI:158508429 with silent change g588a Mouse   Other £110.00
cDNA Clones 14-3-3 epsilonView Data pBabe hygro FLAG-mCherry 14-3-3 epsilon   Human FLAG, mCherry Mammalian £110.00
cDNA Clones 14-3-3 epsilonView Data pcDNA5-FRT/TO NRL-14-3-3 epsilon       Mammalian £110.00
cDNA Clones 14-3-3 epsilonView Data pcDNA5-FRT/TO-mCherry 14-3-3 epsilon     mCherry Mammalian £110.00
cDNA Clones 14-3-3 epsilonView Data pcDNA5-FRT/TO-mCherry 14-3-3 epsilon     mCherry Mammalian £110.00
cDNA Clones 14-3-3 epsilonView Data pEBG-6 14-3-3 epsilon HSU54778   GST Mammalian £110.00
cDNA Clones 14-3-3 epsilonView Data pET28a 14-3-3 epsilon NP_006752.1 Human 6His Bacterial £110.00
cDNA Clones 14-3-3 epsilonView Data pGEX 14-3-3 epsilon Y211/K     GST Bacterial £110.00
cDNA Clones 14-3-3 epsilonView Data pGEX 14-3-3 epsilon E183/K     GST Bacterial £110.00
cDNA Clones 14-3-3 epsilonView Data pGEX 14-3-3 epsilon F199/Y     GST Bacterial £110.00
cDNA Clones 14-3-3 epsilonView Data pGEX-6-14-3-3 epsilon HSU54778 Human GST Bacterial £110.00
cDNA Clones 14-3-3 epsilonView Data pIRES2-mCherry 14-3-3 epsilon/pEGFP NM_006761.4 GI:195546907   GFP Mammalian £110.00
cDNA Clones 14-3-3 epsilonView Data pJET-m-cherry 14-3-3 epsilon       Other £110.00
cDNA Clones 14-3-3 epsilonView Data TOPO-14-3-3 epsilon HSU54778 Human   Other £110.00
cDNA Clones 14-3-3 epsilon (mouse)View Data pBluescript KS+ mouse 14-3-3 epsilon NM_009536.4 GI:226874905 with silent changes c639t and t711c Mouse   Other £110.00
cDNA Clones 14-3-3 etaView Data Hygro-retrovir-FLAG-mCherry 14-3-3 eta   Human FLAG, mCherry Mammalian £110.00
cDNA Clones 14-3-3 etaView Data pcDNA5-FRT/TO-mCherry 14-3-3 eta     mCherry Mammalian £110.00
cDNA Clones 14-3-3 etaView Data pEBG6P-14-3-3 eta NP_003396.1 Human GST Mammalian £110.00
cDNA Clones 14-3-3 etaView Data pET28a 14-3-3 eta NP_003396.1 Human 6His Bacterial £110.00
cDNA Clones 14-3-3 etaView Data pET28a 14-3-3 sigma NP_006133.1 Human 6His Bacterial £110.00
cDNA Clones 14-3-3 etaView Data pGEX6P-1-14-3-3 eta NP_003396.1 Human GST Bacterial £110.00
cDNA Clones 14-3-3 etaView Data pJET-m-cherry 14-3-3 eta (Bgl2-Xho1)       Other £110.00
cDNA Clones 14-3-3 eta (mouse)View Data pBluescript KS+ mouse 14-3-3 eta NM_011738.2 GI:162951868 Mouse   Other £110.00
cDNA Clones 14-3-3 gammaView Data Hygro-retrovir-FLAG-mCherry 14-3-3 gamma   Human FLAG, mCherry Mammalian £110.00
cDNA Clones 14-3-3 gammaView Data pcDNA5-FRT/TO-mCherry 14-3-3 gamma     mCherry Mammalian £110.00
cDNA Clones 14-3-3 gammaView Data pEBG6P-14-3-3 gamma AAH20963.1 Human GST Mammalian £110.00
cDNA Clones 14-3-3 gammaView Data pET28a 14-3-3 gamma AAH20963.1 Human 6His Bacterial £110.00
cDNA Clones 14-3-3 gammaView Data pGEX6P-1-14-3-3 gamma AAH20963.1 Human GST Bacterial £110.00
cDNA Clones 14-3-3 gammaView Data pJET-m-cherry 14-3-3 gamma       Other £110.00
cDNA Clones 14-3-3 gamma (mouse)View Data pBluescript KS+ mouse 14-3-3 gamma NM_018871.3 GI:229577260 Mouse   Other £110.00
cDNA Clones 14-3-3 sigmaView Data Hygro-retrovir-FLAG-mCherry 14-3-3 sigma   Human FLAG, mCherry Mammalian £110.00
cDNA Clones 14-3-3 sigmaView Data pcDNA5-FRT/TO-mCherry 14-3-3 sigma     mCherry Mammalian £110.00
cDNA Clones 14-3-3 sigmaView Data pCMV5-FLAG 14-3-3 sigma AF029081 Human FLAG Mammalian £110.00