LigaTrap®Technologies开发了一种新颖的专有技术亲和力配体组合,专门用于从各种物种中纯化单克隆和多克隆免疫球蛋白。除了物种特异性配体外,优点包括能够在比蛋白质a和G(pH≤3.0)更温和的pH(pH 4.0)下洗脱结合抗体,从而降低pH敏感抗体沉淀和失活的可能性。我们的目标是利用优于目前市场上可用的配体来产生高纯度抗体。LigaTrap®团队对目前市场上的净化产品进行了广泛的并行测试。LigaTrap®团队还扩展了我们的能力,包括我们的专家流程开发和下游优化服务。
LigaTrap®正积极与治疗行业快速发展的技术和平台合作。
IgM Purification Resin and Columns
Part # | Name | Amount | Price |
LT-143KIT |
LigaTrap Human IgM Kit |
1 Kit | $379.00 |
LT-143 |
LigaTrap Human IgM Resin |
5 ml | $699.00 |
LT-143-1x1ml |
LigaTrap® Human IgM Purification Column |
1 ml | $149.00 |
LT-143-1x5ml |
LigaTrap® Human IgM Purification Column |
1 x 5 ml | $699.00 |
LT-143-3x1ml |
LigaTrap® Human IgM Purification Column |
3 x 1 ml | $429.00 |
IgM纯化树脂和色谱柱
IgM的研究始于1937年的报告,即用肺炎球菌多糖超免疫的马产生的抗体比典型的兔γ-球蛋白大得多,分子量为990,000道尔顿。根据其大尺寸,新抗体初被称为γ-巨球蛋白,然后在随后的术语中称为“宏”的IgM-M。
IgM是大的抗体,并且是对抗原初次暴露时出现的一个抗体。就已研究的人类和其他哺乳动物而言,脾脏是产生特定IgM的主要部位,脾脏是负责产生抗体的浆母细胞所在。
IgM单克隆抗体越来越适合用作治疗工具,同时在其研究和诊断应用中仍然很受欢迎。蛋白A和G色谱法继续着生命科学行业提纯IgG,但是蛋白A / G证明不适合IgM纯化。
除了提高产量和纯度,LigaTrap®人IgM纯化树脂(LT-143)还解决了许多与标准亲和配体有关的不足,包括与其他常见纯化方法相关的蛋白水解和重复消毒的挑战。
免责声明:由于可能与其他免疫球蛋白发生交叉反应,因此不建议在血清的纯化应用中使用LigaTrap Human IgA纯化树脂。
IgM产量和纯度
使用IgM纯化树脂的主要竞争者从含有5%FBS的CHO培养基中纯化人IgM。LigaTrap®LT-143树脂使用标准色谱条件。IgM纯化树脂的主要竞争者使用了三种不同的制造商推荐的结合条件。
可比性
SDS页面
LigaTrap纯化试剂盒和树脂产品目录
关于LigaTrap
LigaTrap纯化试剂盒和树脂 方便易用。只需将样品添加到树脂中,混合并离心。LigaTrap®纯化方案不仅易于遵循,而且可以快速简便地回收纯化的抗体,以便在终用途分析中进行筛选。可以同时处理多个样品,从而 快速 筛选抗体的产生。LigaTrap®旋转柱仅需使用移液器和离心机,无需设备。每个旋转柱多可以进行10次纯化,每个试剂盒都可以进行100多次纯化,从而使LigaTrap®成为一种经济高效的抗体纯化方法。
LigaTrap®Technologies是*、新颖且获得的肽/肽配体技术的创新者。我们强大的亲和力配体组合具有更长的生命周期,需要温和的pH洗脱来产生高纯度抗体。
Part # | Name | Amount | Price |
LT-136KIT | LigaTrap Goat IgG Kit | 1 Kit | $294.00 |
LT-136 | LigaTrap Goat IgG Resin | 5 ml | $450.00 |
LT-136-1x1ml | LigaTrap® Goat IgG Purification Column | 1 ml | $100.00 |
LT-136-1x5ml | LigaTrap® Goat IgG Purification Column | 1 x 5 ml | $475.00 |
LT-136-3x1ml | LigaTrap® Goat IgG Purification Column | 3 x 1 ml | $295.00 |
LT-095KIT | LigaTrap Human IgG Kit | 1 Kit | $294.00 |
LT-095 | LigaTrap Human IgG Resin | 5 ml | $450.00 |
LT-095-1x1ml | LigaTrap® Human IgG Purification Column | 1 ml | $100.00 |
LT-095-1x5ml | LigaTrap® Human IgG Purification Column | 1 x 5 ml | $475.00 |
LT-095-3x1ml | LigaTrap® Human IgG Purification Column | 3 x 1 ml | $295.00 |
LT-137KIT | LigaTrap Mouse IgG Kit | 1 Kit | $294.00 |
LT-137 | LigaTrap Mouse IgG Resin | 5 ml | $450.00 |
LT-137-1x1ml | LigaTrap® Mouse IgG Purification Column | 1 ml | $100.00 |
LT-137-1x5ml | LigaTrap® Mouse IgG Purification Column | 1 x 5 ml | $475.00 |
LT-137-3x1ml | LigaTrap® Mouse IgG Purification Column | 3 x 1 ml | $295.00 |
LT-139KIT | LigaTrap Rabbit IgG Kit | 1 Kit | $294.00 |
LT-139 | LigaTrap Rabbit IgG Resin | 5 ml | $450.00 |
LT-139-1x1ml | LigaTrap® Rabbit IgG Purification Column | 1 ml | $100.00 |
LT-139-1x5ml | LigaTrap® Rabbit IgG Purification Column | 1 x 5 ml | $475.00 |
LT-139-3x1ml | LigaTrap® Rabbit IgG Purification Column | 3 x 1 ml | $295.00 |
LT-138KIT | LigaTrap Rat IgG Kit | 1 Kit | $294.00 |
LT-138 | LigaTrap Rat IgG Resin | 5 ml | $450.00 |
LT-138-1x1ml | LigaTrap® Rat IgG Purification Column | 1 ml | $100.00 |
LT-138-1x5ml | LigaTrap® Rat IgG Purification Column | 1 x 5 ml | $475.00 |
LT-138-3x1ml | LigaTrap® Rat IgG Purification Column | 3 x 1 ml | $295.00 |
LT-141KIT | LigaTrap Sheep IgG Kit | 1 Kit | $294.00 |
LT-141 | LigaTrap Sheep IgG Resin | 5 ml | $450.00 |
LT-141-1x1ml | LigaTrap® Sheep IgG Purification Column | 1 ml | $100.00 |
LT-141-1x5ml | LigaTrap® Sheep IgG Purification Column | 1 x 5 ml | $475.00 |
LT-141-3x1ml | LigaTrap® Sheep IgG Purification Column | 3 x 1 ml | $295.00 |
LT-142KIT | LigaTrap Chicken IgY Kit | 1 Kit | $349.00 |
LT-142 | LigaTrap Chicken IgY Resin | 5 ml | $555.00 |
LT-142-1x1ml | LigaTrap® Chicken IgY Purification Column | 1 ml | $125.00 |
LT-142-1x5ml | LigaTrap® Chicken IgY Purification Column | 1 x 5 ml | $595.00 |
LT-142-3x1ml | LigaTrap® Chicken IgY Purification Column | 3 x 1 ml | $365.00 |
LT-143KIT | LigaTrap Human IgM Kit | 1 Kit | $379.00 |
LT-143 | LigaTrap Human IgM Resin | 5 ml | $699.00 |
LT-143-1x1ml | LigaTrap® Human IgM Purification Column | 1 ml | $149.00 |
LT-143-1x5ml | LigaTrap® Human IgM Purification Column | 1 x 5 ml | $699.00 |
LT-143-3x1ml | LigaTrap® Human IgM Purification Column | 3 x 1 ml | $429.00 |
LT-144KIT | LigaTrap Llama IgG Kit | 1 Kit | $294.00 |
LT-144 | LigaTrap Llama IgG Resin | 5 ml | $450.00 |
LT-144-1x1ml | LigaTrap® Llama IgG Purification Column | 1 ml | $100.00 |
LT-144-1x5ml | LigaTrap® Llama IgG Purification Column | 1 x 5 ml | $475.00 |
LT-144-3x1ml | LigaTrap® Llama IgG Purification Columns | 3 x 1 ml | $295.00 |
LT-145KIT | LigaTrap Mouse IgM Kit | 1 Kit | $379.00 |
LT-145 | LigaTrap Mouse IgM Resin | 5 ml | $669.00 |
LT-145-1x1ml | LigaTrap® Mouse IgM Purification Column | 1 ml | $149.00 |
LT-145-1x5ml | LigaTrap® Mouse IgM Purification Column | 1 x 5 ml | $699.00 |
LT-145-3x1ml | LigaTrap® Mouse IgM Purification Column | 3 x 1 ml | $429.00 |
LT-146KIT | LigaTrap Human IgA Kit | 1 Kit | $294.00 |
LT-146 | LigaTrap Human IgA Resin | 5 ml | $450.00 |
LT-146-1x1ml | LigaTrap® Human IgA Purification Column | 1 ml | $100.00 |
LT-146-1x5ml | LigaTrap® Human IgA Purification Column | 1 x 5 ml | $475.00 |
LT-146-3x1ml | LigaTrap® Human IgA Purification Column | 3 x 1 ml | $295.00 |
LT-147Kit | LigaTrap Rat IgM Kit | 1 Kit | $379.00 |
LT-147 | LigaTrap Rat IgM Resin 5ml | 5 ml | $669.00 |
LT-147-1x1ml | LigaTrap® Rat IgM Purification Column | 1 ml | $149.00 |
LT-147-1x5ml | LigaTrap® Rat IgM Purification Column | 1 x 5 ml | $699.00 |
LT-147-3x1ml | LigaTrap® Rat IgM Purification Column | 3 x 1 ml | $429.00 |
BU-123-FP | LigaTrap Elution Buffer | 125ml | $22.00 |
BU-124-FP | LigaTrap Regeneration Buffer | 50ml | $22.00 |
BU-125-FP | LigaTrap Neutralization Buffer | 15ml | $22.00 |
BU-126-FP | LigaTrap Storage Buffer | 50 ml | $22.00 |
BU-131-FP | LigaTrap Sample Diluent 2.0 | 15 ml | $22.00 |
BU-132-FP | LigaTrap Equilibration/Wash Buffer 2.0 | 250ml | $27.00 |
BU-123-500-FP | LigaTrap Elution Buffer | 500ml | $60.00 |
BU-124-500-FP | LigaTrap Regeneration Buffer | 500 ml | $125.00 |
BU-125-250-FP | LigaTrap Neutralization Buffer | 250 ml | $149.00 |
BU-126-500-FP | LigaTrap Storage Buffer | 500 ml. | $125.00 |
BU-131-250-FP | LigaTrap Sample Diluent 2.0 | 250 ml. | $235.00 |
BU-132-1-FP | LigaTrap Equilibration/Wash Buffer 2.0 | 1L | $85.00 |