人类原代细胞一览
推荐的培养基* | 产品 | 接种密度 | 溶解后传代 | 标记物** | 种群加倍 |
---|---|---|---|---|---|
C-22070 | 人类心肌细胞 (HCM) | 10,000 – 15,000 cells per cm² |
P2 | Sarcomeric alpha-Actinin+ Slow muscle myosin+ |
> 15 |
C-27101 | 人类软骨细胞(HCH) | 10,000 – 20,000 cells per cm² |
P2 | Differentiation tested | > 10 |
C-22010 C-22011 |
人类脐静脉内皮细胞(HUVEC) | 5,000 – 10,000 cells per cm² |
P1 | vWF+ CD31+ Dil-Ac-LDL uptake+ |
> 15 |
C-22010 C-22011 |
人类脐静脉内皮细胞(HUVEC) 混合 | 5,000 – 10,000 cells per cm² |
P1 | vWF+ CD31+ Dil-Ac-LDL uptake+ |
> 15 |
C-22010 C-22011 |
人类脐静脉内皮细胞 (HUVEC) 预先筛选 |
5,000 – 10,000 cells per cm² |
P1 | vWF+ CD31+ Dil-Ac-LDL uptake+ |
> 15 |
C-22010 C-22011 |
人类脐动脉内皮细胞 (HUAEC) | 5,000 – 10,000 cells per cm² |
P1 | vWF+ CD31+ Dil-Ac-LDL uptake+ |
> 15 |
C-22020 C-22022 |
人类主动脉内皮细胞 (HAoEC) | 5,000 – 10,000 cells per cm² |
P2 | vWF+ CD31+ Dil-Ac-LDL uptake+ |
> 15 |
C-22020 C-22022 |
人类冠状动脉内皮细胞 (HCAEC) | 5,000 – 10,000 cells per cm² |
P2 | vWF+ CD31+ Dil-Ac-LDL uptake+ |
> 15 |
C-22010 C-22011 |
人类肺动脉内皮细胞 (HPAEC) | 5,000 – 10,000 cells per cm² |
P2 | vWF+ CD31+ Dil-Ac-LDL uptake+ |
> 15 |
C-22010 C-22011 |
人类大隐静脉内皮细胞 (HSaVEC) | 5,000 – 10,000 cells per cm² |
P2 | vWF+ CD31+ Dil-Ac-LDL uptake+ |
> 15 |
C-22020 C-22022 |
人类皮肤微血管内皮细胞 (HDMEC) 青少年包皮 |
10,000 – 20,000 cells per cm² |
P2 | vWF+ CD31+ Dil-Ac-LDL uptake+ |
> 15 |
C-22020 C-22022 |
人类皮肤微血管内皮细胞 (HDMEC) 成年人供者 |
10,000 – 20,000 cells per cm² |
P2 | vWF+ CD31+ Dil-Ac-LDL uptake+ |
> 15 |
C-22020 C-22022 |
人类皮肤微血管内皮细胞 (HDMEC) 预先筛选 |
10,000 – 20,000 cells per cm² |
P2 | vWF+ CD31+ Dil-Ac-LDL uptake+ |
> 15 |
C-22020 | 人类皮肤血液内皮细胞 (HDBEC) 青少年包皮 |
10,000 – 20,000 cells per cm² |
P2 | Podoplanin– Prox-1– CD31+ |
> 15 |
C-22020 | 人类皮肤血液内皮细胞 (HDBEC) 成年人供者 |
10,000 – 20,000 cells per cm² |
P2 | Podoplanin– Prox-1– CD31+ |
> 15 |
C-22020 | 人类皮肤淋巴内皮细胞 (HDLEC) 青少年包皮 |
10,000 – 20,000 cells per cm² |
P2 | Podoplanin– Prox-1– CD31+ |
> 15 |
C-22020 | 人类皮肤淋巴内皮细胞 (HDLEC) 成年人供者 |
10,000 – 20,000 cells per cm² |
P2 | Podoplanin– Prox-1– CD31+ |
> 15 |
C-22020 C-22022 |
人类膀胱微血管内皮细胞 (HBdMEC) | 10,000 – 20,000 cells per cm² |
P2 | vWF+ CD31+ Dil-Ac-LDL uptake+ |
> 15 |
C-22020 C-22022 |
人类心脏微血管内皮细胞 (HCMEC) | 10,000 – 20,000 cells per cm² |
P2 | vWF+ CD31+ Dil-Ac-LDL uptake+ |
> 15 |
C-22020 C-22022 |
人类肺脏微血管内皮细胞 (HPMEC) | 10,000 – 20,000 cells per cm² |
P2 | vWF+ CD31+ Dil-Ac-LDL uptake+ |
> 15 |
C-22020 C-22022 |
人类子宫微血管内皮细胞 (HUtMEC) | 10,000 – 20,000 cells per cm² |
P2 | vWF+ CD31+ Dil-Ac-LDL uptake+ |
> 15 |
C-21060 | 人类鼻部上皮细胞 (HNEpC) | 10,000 – 15,000 cells per cm² |
P2 | Cytokeratin+ | > 15 |
C-21060 | 人类气管上皮细胞 (HTEpC) | 10,000 – 15,000 cells per cm² |
P2 | Cytokeratin+ | > 15 |
C-21060 | 人类支气管上皮细胞 (HBEpC) | 10,000 – 15,000 cells per cm² |
P2 | Cytokeratin+ | > 15 |
C-21070 | 人类小气道上皮细胞 (HSAEpC) | 10,000 – 15,000 cells per cm² |
P2 | Cytokeratin+ | > 15 |
C-21010 | 人类乳腺上皮细胞 (HMEpC) | 10,000 – 15,000 cells per cm² |
P2 | Cytokeratin+ | > 15 |
C-26001 C-26030 |
人类肾脏上皮细胞 (HREpC) | 10,000 – 15,000 cells per cm² |
P2 | Cytokeratin+ | > 15 |
C-26001 C-26030 |
人类肾皮质上皮细胞 (HRCEpC) | 10,000 – 15,000 cells per cm² |
P2 | Cytokeratin+ | > 15 |
C-26040 | 人类胎盘上皮细胞 (HPlEpC) | 10,000 – 15,000 cells per cm² |
P2 | Cytokeratin+ | > 5 |
C-23010 | 正常人类皮肤成纤维细胞 (NHDF) 青少年包皮 |
3,500 – 7,000 cells per cm² |
P2 | CD90+ | > 15 |
C-23020 | 正常人类皮肤成纤维细胞 (NHDF) 成年人供者 |
3,500 – 7,000 cells per cm² |
P2 | CD90+ | > 15 |
C-23020 | 人类肺脏成纤维细胞 (HPF) | 3,500 – 7,000 cells per cm² |
P2 | CD90+ | > 15 |
C-23020 | 人类主动脉外膜成纤维细胞 (HAoAF) | 3,500 – 7,000 cells per cm² |
P2 | CD90+ | > 10 |
C-23025 | 人类心脏成纤维细胞 (HCF) | 3,500 – 7,000 cells per cm² |
P2 | CD90+ Smooth muscle α-actin– Slow muscle myosin– |
> 15 |
C-23020 | 人类子宫成纤维细胞 (HUF) | 3,500 – 7,000 cells per cm² |
P2 | CD90+ | > 15 |
C-23020 | 人类绒毛膜间充质成纤维细胞 (HVMF) | 3,500 – 7,000 cells per cm² |
P2 | CD90+ | > 15 |
C-26501 | 人类毛囊毛乳头细胞 (HFDPC) | 5,000 – 10,000 cells per cm² |
P2 | Alkaline phosphatase+ | > 10 |
C-25010 C-25020 |
人类肝脏细胞 (HHpC) | 150,000 cells per cm² | P0 | ECOD+ 7-HCG/7-HCS+ Albumin+ |
|
C-20011 | 正常人类表皮角质化细胞 (NHEK) 青少年包皮, 单一供者 |
5,000 cells per cm² | P2 | Cytokeratin+ | > 15 |
C-20011 | 正常人类表皮角质化细胞 (NHEK) 青少年包皮,混合 | 5,000 cells per cm² | P2 | Cytokeratin+ | > 15 |
C-20011 | 正常人类表皮角质化细胞 (NHEK) 成年人单一供者 |
5,000 cells per cm² | P2 | Cytokeratin+ | > 15 |
C-20011 | 正常人类表皮角质化细胞 (NHEK) 成年人供者, 混合 |
5,000 cells per cm² | P2 | Cytokeratin+ | > 15 |
C-24010 | 正常人类表皮黑色素细胞 (NHEM) 青少年包皮 |
5,000 – 10,000 cells per cm² |
P2 | Mel-5+ | > 15 |
C-24300 | 正常人类表皮黑色素细胞 (NHEM) 青少年包皮,培养于M2培养基 |
5,000 – 10,000 cells per cm² |
P2 | Mel-5+ | > 15 |
C-24300 | 正常人类表皮黑色素细胞 (NHEM) 成年人供者,培养于M2培养基 |
5,000 – 10,000 cells per cm² |
P2 | Mel-5+ | > 15 |
C-27001 | 人类成骨细胞 (HOB) | 10,000 – 20,000 cells per cm² |
P2 | Alkaline phosphatase+ Mineralization tested |
> 10 |
C-27410 C-27436 C-27438 |
人类白色前成脂肪细胞 (HWP) 皮下脂肪 |
5,000 cells per cm² |
P2 | Differentiation tested | > 10 |
C-27410 C-27436 C-27438 |
人类白色前成脂肪细胞 (HWP) 内脏脂肪 |
5,000 cells per cm² |
P2 | Differentiation tested | > 10 |
C-23060 C-23061 |
人类骨骼肌细胞 (SkMC) |
3,500 – 7,000 cells per cm² |
P2 | Sarcomeric Myosin+ Differentiation tested |
> 15 |
C-22062 | 人类主动脉平滑肌细胞 (HAoSMC) |
7,500 – 10,000 cells per cm² |
P2 | Smooth muscle alpha-Actin+ | > 15 |
C-22062 | 人类冠状动脉平滑肌细胞 (HCASMC) | 7,500 – 10,000 cells per cm² |
P2 | Smooth muscle alpha-Actin+ | > 15 |
C-22062 | 人类肺动脉平滑肌细胞 (HPASMC) |
7,500 – 10,000 cells per cm² |
P2 | Smooth muscle alpha-Actin+ | > 15 |
C-22062 | 人类脐带动脉平滑肌细胞 (HUASMC) | 7,500 – 10,000 cells per cm² |
P2 | Smooth muscle alpha-Actin+ | > 15 |
C-22062 | 人类气管平滑肌细胞 (HTSMC) | 7,500 – 10,000 cells per cm² |
P2 | Smooth muscle alpha-Actin+ | > 15 |
C-22062 | 人类支气管平滑肌细胞(HBSMC) | 7,500 – 10,000 cells per cm² |
P2 | Smooth muscle alpha-Actin+ | > 15 |
C-22062 | 人类膀胱平滑肌细胞 (HBdSMC) | 7,500 – 10,000 cells per cm² |
P2 | Smooth muscle alpha-Actin+ | > 15 |
C-22062 | 人类前列腺平滑肌细胞 (HPrSMC) | 7,500 – 10,000 cells per cm² |
P2 | Smooth muscle alpha-Actin+ | > 15 |
C-22062 | 人类子宫平滑肌细胞 (HUtSMC) |
7,500 – 10,000 cells per cm² |
P2 | Smooth muscle alpha-Actin+ | > 15 |
* 该表格中的培养基为即用型。培养基试剂盒信息,请参见第4章。
** CD 31, Prox-1 和肾小球上皮细胞整合膜蛋白抗体均可以从 PromoKine购买。
- 心肌细胞
- 软骨细胞
- 内皮细胞(大血管)
- 内皮细胞(微血管)
- 上皮细胞
- 成纤维细胞
- 毛囊毛乳头细胞
- 肝细胞