世界*实验材料供应商 Viagen Biotech, Inc.正式上海金畔生物为其中国代理, Viagen Biotech, Inc.在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品,的服务,签约 Viagen Biotech, Inc.就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获
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Viagen Biotech Inc.是创新的DNA提取技术和生命科学研究产品的供应商。ViagenDirectPCR®DNA提取系统是一种单管系统,用于从小鼠尾巴,耳朵片,卵黄囊和培养细胞中快速制备DNA。由Viagen Biotech Inc.的科学家开发的正在申请的组件允许得到的DNA提取物与用于基因分型的基因组PCR相容。生物样品的粗提取物与许多分子生物学级反应(如聚合酶链反应(PCR))不相容,部分原因是粗提物中含有抑制剂。DirectPCR试剂不仅介导生物样品的快速溶解,而且还含有有效抑制粗裂解物对PCR扩增的抑制活性的抑制剂,同时zui大限度地保持了释放的基因组DNA的完整性。该过程*消除了任何溶液转移或开管步骤,节省了大量的时间和精力。DirectPCR试剂每尾只花费26美分,可以节省任何使用量。
Viagenbiotech致力于简便快速提取和制备核酸(DNA / RNA),以批量、快速进行基因分析。公司拥有的技术不仅加快样品的裂解,而且降低了粗提物中PCR抑制因素,因而使DNA提取与PCR基因分析兼容,样品经一步消化后即可进行PCR扩增,并且保证了基因组的完整性。
Proteinase K Solution 5ml (20mg/ml)
Availability: In stock
Quick Overview
Genomic PCR Grade Proteinase K Solution
Catalog No. 501-PK
Stable for 12 months at -20℃ / Ships Refrigerated Fedex Overnight
Details
Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Proteinase K is classified as a serine protease. The smallest peptide to be hydrolysed is a tetrapeptide.
DNA Extraction lysis reagent for DNA tests & genotyping: The Proteinase K solution is useful when a small amount of biological samples are processed. Add the enzyme solution to DirectPCR reagents at the final concentration of 0.5-1.0 mg/ml (25-50 μl Proteinase K solution per 1 ml DirectPCR reagent). Follow the instruction manuals for DirectPCR Reagent Protocol/Manual for each blood, blood stain, buccal swab, hair root DNA genotyping. Once diluted in DirectPCR reagents, Proteinase K is stable for ~24 hrs.
Other applications: Proteinase K digests native proteins very effectively. It can therefore be used to remove DNases and RNases when isolating DNA and RNA from tissues or cells.
Usage Conditions:
• Proteinase K is active over a wide pH range—optimal activity between 6.5 and 9.5
• Under denaturing conditions—e.g., in the presence of SDS or urea
• In the presence of metal chelating agents—e.g., EDTA
• At high temperatures—optimum digestion temperature is 65°C
Unit Definition: One unit of the enzyme liberates folin-positive amino acids and peptides corresponding to 1μmol tyrosine in 1min at 37°C using denatured hemoglobin as substrate.
Quality Control: Tested for the absence of endo-, exodeoxyribonucleases and ribonucleases
部分产品报价如下:
货号 | 品名 | 规格 | 价格 | 品牌 |
101-T | DirectPCR Lysis Reagent (mouse tail) | 50mL | 1160 | Viagen Biotech, Inc. |
102-T | DirectPCR Lysis Reagent (mouse tail) | 100mL | 2040 | Viagen Biotech, Inc. |
201-Y | DirectPCR Lysis Reagent (yolk sac) | 50mL | 1160 | Viagen Biotech, Inc. |
202-Y | DirectPCR Lysis Reagent (yolk sac) | 100mL | 2040 | Viagen Biotech, Inc. |
301-C | DirectPCR Lysis Reagent (cell) | 50mL | 1160 | Viagen Biotech, Inc. |
302-C | DirectPCR Lysis Reagent (cell) | 100mL | 2040 | Viagen Biotech, Inc. |
401-E | DirectPCR Lysis Reagent (mouse ear) | 25mL | 1160 | Viagen Biotech, Inc. |
402-E | DirectPCR Lysis Reagent (mouse ear) | 50mL | 2040 | Viagen Biotech, Inc. |
21141 | Redsafe Nucleic Acid Staining Solution | 1ml | 1180 | Viagen Biotech, Inc. |
505-PKP | Genomic PCR Grade Proteinase K Powder | 100mg | 1300 | Viagen Biotech, Inc. |
506-PKP | Genomic PCR Grade Proteinase K Powder | 200mg | 2200 | Viagen Biotech, Inc. |
507-PKP | Genomic PCR Grade Proteinase K Powder | 500mg | 4700 | Viagen Biotech, Inc. |
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6:我们*代理的品牌有:Antibody Research Corporation,arcticzymes,Biorelevant,AmberGen, Inc. ,clemente-associates,clodronaiposomes,Columbia Biosciences,enzyme research,Gene Bridges GmbH,Genovis,AmberGen, Inc. Biotechnology GmbH,Haematologic Technologies HTI Haemtech,hookelabs,Immudex,Innovative Research of America,inspiralis ,List Biological Laboratories, Inc.,lumafluor,Microsurfaces,multiplicom,nanotools,Pel-Freez Biologicals,pentapharm,progen,Protein Ark,QA-Bio,Inc,QA-Bio,IncQuickZyme Biosciences,Teknova,TriLink BioTechnologies, Inc.,Zyagen Laboratories 等
7:我们还是invitrogen,qiagen,Midland BioProducts Corporationam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知名*批发,欢迎合作。