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lucerna DFHBI-1T 大量现货
名称：FHBI-1T
英文全名：（(Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one )
用途：与目的RNA结合后被激活发射荧光。
分子式：C13H9F5N2O2
分子量：320.21
结构式：
CAS号码：1539318-36-0
吸收与发射光谱：
Absorption and fluorescence emission spectra of Spinach2TM/DFHBI-1T in pH 7.4 buffer.
状态：冻干粉
保存：-20度 避光保存2年
纯度：>95% by HPLC
与DFHBI荧光强度比较
STORAGE CONDITIONS: Stable in the dark for 2 year at -20 ºC.
QUALITY ASSURANCE: Products are analyzed by 1H NMR and LC-MS and provided at purity of >95% by HPLC.
USAGE STATEMENT: This product is intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Due to the highly specific nature of fluorophores and aptamers, we cannot predict or be held responsible with respect to how LucernaTM products will behave in its customers' systems. Researchers using LucernaTM products should conduct optimization studies to achieve the optimal result possible for their intended application.
LICENSING: Cornell University has filed patents titled “RNA sequences that switch on the fluorescence of small molecules and their use in detection of RNA in vitro and in cells” and “novel methods for RNA detection and quantification.” All commercial users should contact Cornell University for a use license.
DFHBI-1T IMAGES
A. Live-cell imaging of a COS7 cell expressing CGG60-Spinach2TM in the presence of either 20 µM DFHBI or DFHBI-1T. Images of the same cell were acquired using a 100 msec exposure with a GFP filter set. B. Quantification of average DFHBI-1T brightness of 10 cells expressing CGG60-SpinachTM normalized to the brightness of DFHBI.
参考文献：
Song W, Strack RL, Svensen N, Jaffrey SR. 2014. Plug-and-Play Fluorophores Extend the Spectral Properties of Spinach. JACS136(4):1198-1201.
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