世界*实验材料供应商 Addgene上海金畔生物为其中国代理， Addgene在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品，的服务， Addgene就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获
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Addgene是一个性的非盈利性储存库，旨在帮助科学家共享质粒。质粒是生命科学中常用的基于DNA的研究试剂。当科学家发表研究论文时，他们将相关的质粒沉积在Addgene。然后，当其他科学家读了这本出版物时，他们可以轻松访问进行未来实验所需的质粒。在Addgene之前，科学家的任务是不断向每个新的请求科学家发送质粒。现在，科学家将他们的质粒运送到了Addgene一次，我们照顾质量控制，MTA合规性，运输和记录保存。
对于寻求使用质粒的科学家，Addgene提供了一个可搜索的高质量质粒数据库，合并库和质粒试剂盒，价格合理。Addgene存储库中的所有质粒均按照质量控制目的进行排序，并在条形码到达我们的设施之前跟踪条形码，直到它们装运。科学家可以 通过Addgene的在线订购系统请求质粒。到2016年，Addgene还为科学家提供了常用的质粒即可使用的AAV和慢病毒制剂。
Addgene还正在建立各种教育资源，包括协议，博客文章和电子书。我们的质粒101和101 CRISPR博客系列旨在帮助各级科学家了解 更多关于分子生物学，克隆，基因工程等。Addgene的团队还为科学家提供实时质粒排除问题 – 无论是关于质粒序列突变的问题，还是帮助选择*实验质粒。通过提供这些服务，Addgene的目标是为世界各地的研究和发现创造一个持久的资源。
部分产品信息如下：
Plasmids
- pPAGFP-C1 (Plasmid #11910)
Insert: PAGFP Aequorea victoria
Tags: None
Expression: Mammalian
Mutation: These plasmids are essentiall…
Use:
Deposited by: Jennifer Lippincott-Schwartz
- pPAGFP-N1 (Plasmid #11909)
Insert: pPAGFP-N1 Aequorea victoria
Tags: None
Expression: Mammalian
Mutation: These plasmids are essentiall…
Use:
Deposited by: Jennifer Lippincott-Schwartz
- pCXWB-EBNA1 (Plasmid #37624)
PurposeNon-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids
Insert: EBNA-1 ( BKRF1 human herpesvirus 4 )
Tags: None
Expression: Mammalian
Mutation: None
Use:
Deposited by: Shinya Yamanaka
- pMD2.G (Plasmid #12259)
PurposeVSV-G envelope expressing plasmid
Insert: VSV G
Tags: None
Expression: Mammalian
Mutation: None
Use: Lentiviral ; Envelope
Deposited by: Didier Trono
- pSL-MS2-12X (Plasmid #27119)
Insert: MS2 binding sites (12)
Tags: None
Expression: Bacterial
Mutation: None
Use:
Deposited by: Robert Singer
- pcDNA3.1-GFP(1-10) (Plasmid #70219)
PurposeExpresses GFP(1-10) in mammalian cells
Insert: GFP(1-10) Synthetic
Tags: None
Expression: Mammalian
Mutation: None
Use:
Deposited by: Bo Huang
- pC1-HyPer-3 (Plasmid #42131)
Insert: HYPER 3 Synthetic
Tags: None
Expression: Mammalian
Mutation: None
Use:
Deposited by: Vsevolod Belousov
- AAVS1 SA-2A-puro-pA donor (Plasmid #22075)
PurposeAAV zinc finger donor vector for genomic targeting
Insert: SA-Puro Human
Tags: None
Expression:
Mutation: None
Use: ZFN donor
Deposited by: Rudolf Jaenisch
- pAAV-Ef1a-DIO EYFP (Plasmid #27056)
Insert: EYFP Human
Tags: None
Expression: Mammalian
Mutation: None
Use: AAV
Deposited by: Karl Deisseroth
- pMS2-GFP (Plasmid #27121)
Insert: MS2 bacteriophage MS2
Tags: SV40 NLS, HA, EGFP
Expression: Mammalian
Mutation: None
Use:
Deposited by: Robert Singer
- gag/pol (Plasmid #14887)
Insert: gag / pol
Tags: None
Expression: Mammalian
Mutation: None
Use: Retroviral
Deposited by: Tannishtha Reya
- pCXLE-EGFP (Plasmid #27082)
PurposeIntegration-free (episomal) expression of EGFP
Insert: eGFP
Tags: None
Expression: Mammalian
Mutation: None
Use:
Deposited by: Shinya Yamanaka
- LV-Cre pLKO.1 (Plasmid #25997)
Insert: Cre recombinase bacteriophage
Tags: None
Expression: Mammalian
Mutation: None
Use: Lentiviral
Deposited by: Elaine Fuchs
- pEB-Tg (Plasmid #28220)
Insert: SV40 large T SV40
Tags: None
Expression: Mammalian
Mutation: None
Use:
Deposited by: Linzhao Cheng
- mCerulean C1 (Plasmid #27796)
Insert: mCerulean C1
Tags: None
Expression: Mammalian
Mutation: None
Use:
Deposited by: Steven Vogel
- pGFP-Rab8A (Plasmid #24898)
Insert: hRab8A Human
Tags: GFP
Expression: Mammalian
Mutation: None
Use:
Deposited by: Maxence Nachury
- pLKO.1 puro GFP siRNA (Plasmid #12273)
Insert: GFP siRNA
Tags: None
Expression: Mammalian
Mutation: None
Use: Lentiviral, RNAi
Deposited by: Bob Weinberg
- Cas9m4-VP64 (Plasmid #47319)
PurposeCas9m4 Activator
Insert: Cas9m4-VP64
Tags: None
Expression:
Mutation: None
Use: CRISPR
Deposited by: George Church
- pUAS-luc2 (Plasmid #24343)
Insert: Luciferase
Tags: 5XGAL4
Expression: Mammalian
Mutation: None
Use:
Deposited by: Liqun Luo
- LV-Lac (Plasmid #12108)
Insert: LacZ
Tags: None
Expression: Mammalian
Mutation: None
Use: Lentiviral
Deposited by: Inder Verma