DAPI染色液(即用型)

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上海金畔生物科技有限公司提供DAPI染色液(即用型) ，欢迎访问官网了解更多产品信息。

产品编号 C02-04002
英文名称 DAPI solution (Nuclear Labeling)
中文名称 DAPI染色液(即用型)
别    名 Nuclear Labeling Kit; DAPI solution(ready-to-use); DAPI Staining Solution;   DAPI溶液(即用型) 10ug/ml; DAPI染液(即用型); DAPI溶液（即用型）; 核染色试剂盒; 核染色试剂盒(即用型); 核染色试剂盒（即用型）; DAPI(4’,6-二脒基-2-苯基吲哚二盐酸盐); 4’,6-二脒基-2-苯基吲哚二盐酸盐;
DAPI染色液(即用型)
Specific References  (57)     |     C02-04002 has been referenced in 57 publications.
[IF=16.744] Lubin Zhou. et al. A self-pumping dressing with in situ modification of non-woven fabric for promoting diabetic wound healing. CHEM ENG J. 2022 Dec;:141108  
PubMed:10.1016/j.cej.2022.141108

[IF=9.995] Zhifang An. et al. A homotetrameric hemoglobin expressed in alveolar epithelial cells increases blood oxygenation in high-altitude plateau pika (Ochotona curzoniae). CELL REP. 2022 Oct;41:111446  Other ;  Other.  
PubMed:36198267

[IF=9.685] Zhang, Yuan. et al. Downregulating PDPK1 and taking phillyrin as PDPK1-targeting drug protect hepatocytes from alcoholic steatohepatitis by promoting autophagy. CELL DEATH DIS. 2022 Nov;13(11):1-14  
PubMed:36418288

[IF=9.587] Shi Yunhong. et al. N,N-Dimethyl-3β-hydroxycholenamide attenuates neuronal death and retinal inflammation in retinal ischemia/reperfusion injury by inhibiting Ninjurin 1. J NEUROINFLAMM. 2023 Dec;20(1):1-18  
PubMed:37029422

[IF=9.423] Yu Pang. et al. Bladder epithelial cell phosphate transporter inhibition protects mice against uropathogenic Escherichia coli infection. CELL REP. 2022 Apr;39:110698  Other ;  Other.  
PubMed:35443182

[IF=9.304] Jiantuan Xiong. et al. miR-195-3p alleviates homocysteine-mediated atherosclerosis by targeting IL-31 through its epigenetics modifications. 2021 Sep 30  IF ;  Mouse.  
PubMed:34592792

[IF=7.79] Zhaoyan Yang. et al. Water-dispersed CsPbBr3 Nanocrystals for Single Molecule Localization Microscopy with High Location Accuracy for Targeted Bioimaging. Nanoscale. 2022 Mar;:  Other ;  Other.  
PubMed:35415728

[IF=7.675] Jiahui Tang. et al. Increased Mobile Zinc Regulates Retinal Ganglion Cell Survival via Activating Mitochondrial OMA1 and Integrated Stress Response. ANTIOXIDANTS-BASEL. 2022 Oct;11(10):2001  Other ;  Other.  
PubMed:36290724

[IF=7.376] Li-Ming Yu. et al. Activation of PKG-CREB-KLF15 by melatonin attenuates Angiotensin II-induced vulnerability to atrial fibrillation via enhancing branched-chain amino acids catabolism. Free Radical Bio Med. 2021 Dec;:  IF ;  Mouse.  
PubMed:34864165

[IF=7.367] Yitian Du. et al. Engineered Microglia Potentiate the Action of Drugs against Glioma Through Extracellular Vesicles and Tunneling Nanotubes. 2021 Feb 28  IF,IHC ;  
PubMed:33644993

[IF=7.243] Junping Liu. et al. JAG1 enhances angiogenesis in triple-negative breast cancer through promoting the secretion of exosomal lncRNA MALAT1. GENES DIS. 2022 Aug;:  Other ;  Other.  
PubMed:10.1016/j.gendis.2022.07.006

[IF=6.633] Li-Ming Yu. et al. Icariin attenuates excessive alcohol consumption-induced susceptibility to atrial fibrillation through SIRT3 signaling. BBA-MOL BASIS DIS. 2022 Oct;1868:166483  
PubMed:35798229

[IF=6.63] Guo, Zhi-chen. et al. Porphyromonas gingivalis promotes the progression of oral squamous cell carcinoma by activating the neutrophil chemotaxis in the tumour microenvironment. CANCER IMMUNOL IMMUN. 2022 Dec;:1-17  
PubMed:36513851

[IF=6.543] Yu Xing. et al. Changes of Functional, Morphological, and Inflammatory Reactions in Spontaneous Peripheral Nerve Reinnervation after Thermal Injury. Oxid Med Cell Longev. 2022;2022:9927602  Other ;  Other.  
PubMed:35154578

[IF=5.895] Xinling Wang. et al. Enhancement of BCAT2-Mediated Valine Catabolism Stimulates β-Casein Synthesis via the AMPK-mTOR Signaling Axis in Bovine Mammary Epithelial Cells. J AGR FOOD CHEM. 2022;XXXX(XXX):XXX-XXX  Other ;  Other.  
PubMed:35916279

[IF=5.895] Sheng-Zhong Han. et al. miR-455-3p Is Negatively Regulated by Myostatin in Skeletal Muscle and Promotes Myoblast Differentiation. J AGR FOOD CHEM. 2022;70(33):10121–10133  Other ;  Other.  
PubMed:35960196

[IF=5.561] Yu, Li-Ming. et al. Inhibition of ferroptosis by icariin treatment attenuates excessive ethanol consumption-induced atrial remodeling and susceptibility to atrial fibrillation, role of SIRT1. APOPTOSIS. 2023 Jan;:1-20  
PubMed:36708428

[IF=5.555] Pengsen Wu. et al. IL-11 Is Elevated and Drives the Profibrotic Phenotype Transition of Orbital Fibroblasts in Thyroid-Associated Ophthalmopathy. Front Endocrinol. 2022; 13: 846106  Other ;  Other.  
PubMed:35273577

[IF=5.552] Zhou L et al. Levo-corydalmine Attenuates Vincristine-Induced Neuropathic Pain in Mice by Upregulating the Nrf2/HO-1/CO Pathway to Inhibit Connexin 43 Expression. Neurotherapeutics. 2019 Oct 15.  IHF ;  
PubMed:31617070

[IF=5.279] Min-Min Hu. et al. Sesamol Reverses Myofiber-Type Conversion in Obese States via Activating the SIRT1/AMPK Signal Pathway. J Agr Food Chem. 2022;70(7):2253–2264  Other ;  Other.  
PubMed:35166533

[IF=5.085] Shuang-Yan Chang. et al. miR-320 regulates myogenesis by targeting growth factor receptor-bound protein-2 and ameliorates myotubes atrophy. INT J BIOCHEM CELL B. 2022 Jun;147:106212  Other ;  Other.  
PubMed:10.1016/j.biocel.2022.106212

[IF=5.08] Ye, Fan. et al. Rutin ameliorates inflammatory pain by inhibiting P2X7 receptor in mast cells. J PHYSIOL BIOCHEM. 2022 Dec;:1-9  
PubMed:36512286

[IF=4.831] Lu Qi. et al. A newly identified small molecular compound acts as a protein kinase inhibitor to suppress metastasis of colorectal cancer. Bioorg Chem. 2021 Feb;107:104625  
PubMed:33454506

[IF=4.803] Xing Du. et al. The mevalonate pathway promotes the metastasis of osteosarcoma by regulating YAP1 activity via RhoA. Genes Dis. 2020 Nov;:  IF ;  
PubMed:10.1016/j.gendis.2020.11.009

[IF=4.627] Xiong X et al. Extracellular matrix derived from human urine-derived stem cells enhances the expansion, adhesion, spreading, and differentiation of human periodontal ligament stem cells. Stem Cell Res Ther. 2019 Dec 18;10(1):396.  Other ;  
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[IF=4.599] Jimei Li. et al. A New Homotetramer Hemoglobin in the Pulmonary Surfactant of Plateau Zokors (Myospalax Baileyi). Front Genet. 2022; 13: 824049  Other ;  Other.  
PubMed:35368669

[IF=4.529] Zha Zheng. et al. Study on the Anti-demyelination Mechanism of Bu-Shen-Yi-Sui Capsule in the Central Nervous System Based on Network Pharmacology and Experimental Verification. MEDIAT INFLAMM. 2022;2022:9241261  
PubMed:35865997

[IF=4.473] Xudong Guo. et al. ARID3A plays a key regulatory role in palmitic acid-stimulated milk fat synthesis in mouse mammary epithelial cells. CELL BIOL INT. 2023 Feb;:  
PubMed:36786478

[IF=4.268] Lei Yu. et al. Oxocrebanine: A Novel Dual Topoisomerase inhibitor, Suppressed the Proliferation of Breast Cancer Cells MCF-7 by Inducing DNA Damage and Mitotic Arrest. Phytomedicine. 2021 Apr;84:153504  other ;  
PubMed:33611211

[IF=4.249] Zhou L et al. Levo-corydalmine alleviates vincristine-induced neuropathic pain in mice by inhibiting an NF-kappa B-dependent CXCL1/CXCR6 signaling pathway. Neuropharmacology. 2018 Jun;135:34-47.  IF ;  
PubMed:29518397

保存条件 Store at 2-8℃ for one year.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 基本信息：
λ Ex/λ Em（结合 DNA）= 360/460 nm
CAS 号：28718-90-3
分子量：350.25
分子式：C16H17Cl2N5

产品简介：
DAPI即4′,6-二脒基-2-苯基吲哚，能与DNA强力结合的荧光染料，常用与荧光显微镜及共聚焦观测。DAPI可以透过完整的细胞膜，用于活细胞和固定细胞的染色。分子式为C16H15N5•2HCl ，分子量为350.25。
DAPI可以穿透细胞膜与细胞核中的双链DNA结合而发挥标记的作用，可以产生比DAPI自身强20多倍的荧光。显微镜下可以看到显蓝色荧光的细胞，荧光显微镜观察细胞标记的效率高(几乎为100 %) ，且对活细胞无毒副作用。DAPI染色常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。DAPI也常用于普通的细胞核染色以及某些特定情况下的双链DNA染色。细胞经热激处理后用DAPI染色3分钟，在荧光显微镜下可以看到到细胞核的形态变化。
DAPI的最大激发波长为340nm，最大发射波长为488nm， DAPI和双链DNA结合后，最大激发波长为360nm，最大发射波长为460nm。
本DAPI染色液(DAPI Staining Solution)可以直接用于固定细胞或组织的细胞核染色。

使用方法：
1. 对于固定后的细胞或组织样品，适当洗涤去除固定剂。如果需要进行免疫荧光染色，可先进行免疫荧光染色，染完毕后再进行DAPI染色。如果不需要进行其它染色，则直接进行DAPI染色。
2. 对于贴壁细胞或组织切片，加入少量DAPI染色液，覆盖住样品即可。对于悬浮细胞，至少加入待染色样品体积3倍的色液，混匀。
3. 室温孵育5-10分钟。
4. 吸除DAPI染色液，用TBST、PBS或生理盐水洗涤2-3次，每次3-5分钟，洗掉未结合的DAPI。
5. 用带有360nm激发波长，460nm发射波长的滤光片的荧光显微镜观察细胞。

注意事项：
1. 荧光染料都存在淬灭的问题，建议染色后尽量当天完成检测。
2. 为减缓荧光淬灭可以使用抗荧光淬灭封片液（产品编号：C02-04003）。
3. DAPI对人体有一定刺激性，请注意适当防护，为了您的安全和健康，使用时戴一次性手套操作。
产品图片
DAPI染色液(即用型)
小鼠肺 石蜡切片 细胞核呈蓝色