细胞增殖及毒性检测试剂盒

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上海金畔生物科技有限公司提供细胞增殖及毒性检测试剂盒 ，欢迎访问官网了解更多产品信息。

产品编号 BA00208
英文名称 Cell Counting Kit-8
中文名称 细胞增殖及毒性检测试剂盒
别    名 Cell Counting Kit-8 (CCK-8); CCK-8; WST-8; Enhanced Cell Counting Kit-8; CCK-8 Cell Proliferation and Cytotoxicity Assay Kit; CCK-8 WST-8; Cell Counting Kit – 8;   CCK-8细胞增殖及毒性检测试剂盒; CCK8试剂盒; CCK-8/WST-8试剂盒; CCK-8细胞活力检测试剂盒; 细胞计数试剂盒-8（CCK-8）; CCK-8试剂盒(细胞增殖及毒性检测试剂盒); Cell Counting Kit-8（CCK-8试剂盒）; 细胞计数试剂盒-8; Cell Counting Kit-8(CCK-8)细胞活性检测试剂盒; 细胞活性检测试剂盒;
细胞增殖及毒性检测试剂盒
Specific References  (99)     |     BA00208 has been referenced in 99 publications.
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保存条件 Protect from light. Store at 2-8℃ for one year. Store at -20℃ for two year.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Cell Counting Kit-8，即增强型 CCK-8 试剂盒，是一种基于 WST-8 而广泛应用于细胞增殖和细胞毒性的快速、高灵敏度、无放射性的比色检测试剂盒。WST-8 在电子耦合试剂存在的情况下，可以被线粒体内的一些脱氢酶还原生成橙黄色的 formazan，且细胞增殖越多越快，则颜色越深；细胞毒性越大，则颜色越浅。对于同样的细胞，颜色的深浅 (生成的 formazan 量) 和细胞数目呈线性关系。

WST-8 是 MTT 的一种升级替代产品，和 MTT 或其它 MTT 类似产品，如 XTT、MTS 等相比有明显的优点。第一，MTT 被线粒体内的一些脱氢酶还原生成的 formazan 不是水溶性的，需要有特定的溶剂来溶解；而 WST-8 和 XTT、MTS 产生的 formazan 都是水溶性的，可以省去后续的溶解步骤。第二，WST-8 产生的 formazan 比 XTT 和 MTS 产生的 formazan 更易溶解。第三，WST-8 比 XTT 和 MTS更加稳定，使实验结果更可靠。第四，WST-8 和 MTT、XTT 等相比线性范围更宽，灵敏度更高，并且更加稳定。

WST-8 对细胞无明显毒性，可以直接加入到细胞样品中，无需预配各种成分；显色后，可以在不同时间反复用酶标仪读板，检测时间更加灵活，便于确定最佳测定时间；酚红和血清对本试剂盒的测定无明显影响。
产品图片
细胞增殖及毒性检测试剂盒
Bioss CCK-8产品(BA00208)与国际G品牌对比图:图中左侧样本为SP2/O的检测数据,右侧样本为3T3的检测数据.Bioss产品稳定性与特异性与国际G品牌同等优质.

细胞增殖及毒性检测试剂盒
Bioss CCK-8产品(BA00208)与国际D品牌对比图:图中左侧样本为SP2/O的检测数据,右侧样本为3T3的检测数据.Bioss产品稳定性与特异性与国际D品牌同等优质.