HAT培养基添加剂 (50×)

发表于由

上海金畔生物科技有限公司提供HAT培养基添加剂 (50×) ，欢迎访问官网了解更多产品信息。

产品编号 C7102
英文名称 HAT Supplement (50×)
中文名称 HAT培养基添加剂 (50×)
别    名 HAT Media Supplement (50×) Hybri-Max™; H0262 (Sigma-Aldrich); HAT Media Supplement(50×); HAT Supplement, Hypoxanthine-Aminopterin-Thymidin (HAT) media supplement  HAT补充剂; 次黄嘌呤氨基蝶呤胸苷（HAT）培养基补充剂; HAT添加剂;
保存条件 Store at -20℃.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍

HAT Supplement is a liquid mixture of sodium hypoxanthine, aminopterin and thymidine. After dilution, HAT is suitable for use as a post-fusion selective medium to eliminate unfused or self-fused HGPRT-myeloma cells.

γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma;

Application:
HAT Media Supplement (50×) Hybri-Max™ has been used:
• in the selection and validation of hypoxanthine-guanine phosophoribosyltransferase (HPRT)-deficient clones.
• in the generation of mAbs (monoclonal antibodies).
• in the luciferase-based bioassay (LUC) assay.

Reconstitution:
Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 0.4 μM aminopterin, 16 μM thymidine.