RIPA裂解液(强)

上海金畔生物科技有限公司提供RIPA裂解液(强) ,欢迎访问官网了解更多产品信息。

产品编号 C5029
英文名称 RIPA Lysis buffer (strong)
中文名称 RIPA裂解液(强)
别    名 RIPA buffer(high); RIPA buffer(strong); RIPA buffer; lysis buffer;   蛋白提取试剂盒; 高效组织细胞裂解液; 高效RIPA裂解液(组织/细胞); 蛋白裂解液(组织/细胞);
RIPA裂解液(强)
Specific References  (7)     |     C5029 has been referenced in 7 publications.
[IF=6.107] Wei-Yun Shen. et al. Platelets reprogram monocyte functions by secreting MMP-9 to benefit postoperative outcomes following acute aortic dissection. ISCIENCE. 2023 May;:106805  
PubMed:37250799

[IF=5.732] Shen, Weiyun. et al. Infiltrating circulating monocytes provide an important source of BMP4 at the early stage of spinal cord injury. DIS MODEL MECH. 2023 Jan;16(1):  
PubMed:36518009

[IF=5.121] Huang Sida. et al. A non-coding variant in 5’ untranslated region drove up-regulation of Pseudo-kinase EPHA10 and caused non-syndromic hearing loss in humans. HUM MOL GENET. 2022 Sep;:  Other ;  Other.  
PubMed:36048850

[IF=4.848] Han Qi. et al. LINC00337 Regulates KLF5 and Maintains Stem-Cell Like Traits of Cervical Cancer Cells by Modulating miR-145. Front Oncol. 2020 Aug;10:1433  other ;  
PubMed:32923396

[IF=4.225] Yaohua Fan. et al. Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance. Front Pharmacol. 2021; 12: 632602  Other ;  
PubMed:33967768

[IF=3.869] Liang Yu. et al. METTL3-Mediated m6A Methylation Regulates Muscle Stem Cells and Muscle Regeneration by Notch Signaling Pathway. Stem Cells Int. 2021;2021:9955691  Other ;  
PubMed:34093712

[IF=3.423] Wenfeng Li. et al. Shikonin improves pulmonary vascular remodeling in monocrotaline‑induced pulmonary arterial hypertension via regulation of PKM2. MOL MED REP. 2023 Mar;27(3):1-10  
PubMed:36734266

保存条件 -20℃保存,有效期12个月。
产品介绍 RIPA裂解液是一种传统的细胞组织快速裂解液,裂解得到的蛋白样品可用于常规的 Western、IP 等实验。蛋白样品可用BCA Protein Assay Kit (C05-02001) 测定蛋白浓度。由于含有较高浓度的去污剂,不能用Bradford法测定RIPA裂解后的蛋白浓度。RIPA 裂解液种类很多,根据其裂解液的强度不同为强、中、弱三类。为保证实验结果请根据检测样本与检测目的的不同选择适当的裂解液。请参考附件(小助手):各种裂解液的主要特点和差异。
RIPA裂解液(强)(Powerful RIPA Lysis Buffer)主要由50mM Tris(pH 7.4),150mM NaCl,1% Triton X-100,1% sodium deoxycholate,0.1% SDS、EDTA 等组成,并含有多种蛋白酶抑制剂成分,可以有效抑制蛋白的降解,维持原有的蛋白间相互作用。
含有PMSF(100mM, 100×)一支。
本产品仅用于科研领域,不用于临床诊断。