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Human Factor VIIa

HFVIIa             Human Factor VIIa

Prepared from purified Human Factor VII using Human Factor XIIa. The Factor Xlla is removed using affinity chromatography. Purity is determined by SDS-PAGE. Human Factor VIIa reduces to 29,500 and 23,500 with the addition of 2-mercaptoethanol. Activity is determined via clotting assay.  Factor Vlla, in the presence of calcium ions and Tissue factor, activates Factors IX and X to their enzymatically active forms, Factor IXa and Xa.

Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/pH 7.4
*Extinction Coefficient （1%） = 13.9
Molecular Weight = 50,000 daltons

Also available: des-Gla-Human Factor VIIa and 
Factor VIIa inactivated