pCMV-VSV-G载体序列说明书
型号 | 载体名称 | 出品公司 | 载体用途 |
VMA0369 | pCMV-VSV-G | Addgene | 慢病毒载体 |
5′ sequencing primer: T7
Vector for constitutive expression of VSV-G glycoprotein. Also known as pHCMV-G or pHCMV-VSV-G.
Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. pCMV-VSV-G expresses the G glycoprotein of the vesicular stomatitis virus (VSV-G) under the control of the CMV immediate-early promoter. VSV-G is used in pseudotyping of Moloney Murine Leukenia Virus(MMLV)-based retroviral vectors by mediating viral entry. VSV-G interacts with phospholipidcomponents of the target cell membrane and fosters the fusion of viral and cellular membranes. VSV-G does not require a cell surface receptor and can serve as a surrogate viral envelope protein.
The vector contains the ampicillin-resistance gene for propagation and antibiotic selection in bacteria.