Backbone size （bp）: 7567
Modifications to Backbone: EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. EGFP and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
Cloning site 5′: HpaI
Cloning site 3′: XhoI
5′ sequencing primer: mU6-F

pSicoR allows for conditional （Cre-Lox）, stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS OFF shRNA expression.