pSilencer1.0
型号 | 载体名称 | 出品公司 | 载体用途 |
VRA0337 | pSilencer1.0 | Ambion | RNAi载体 |
One of the biggest challenges in the use of RNA interference (RNAi) to
study gene function in mammalian systems is delivering the small interfering RNAs (siRNA) or siRNA expression vector into cells. The
pSilencer™ ade.0-CMV System employs adenoviral mediated gene
transfer—a well characterized and useful tool for the delivery of DNA
sequences both in vivo and in vitro. Once inside a mammalian cell, a
modified CMV promoter drives the expression of a hairpin siRNA for
RNAi studies. Using this kit, researchers can produce a replication-deficient adenovirus containing a hairpin siRNA template that targets their
gene of interest. It has been shown that adenoviral vectors are capable of
expressing functional siRNAs that enter the RNAi pathway and reduce
target gene expression in both tissue culture cells and animals (Xia 2002).