pSilencer1.0载体说明书


 pSilencer1.0

型号 载体名称 出品公司 载体用途
VRA0337 pSilencer1.0 Ambion RNAi载体

One of the biggest challenges in the use of RNA interference (RNAi) to

study gene function in mammalian systems is delivering the small interfering RNAs (siRNA) or siRNA expression vector into cells. The

pSilencer™ ade.0-CMV System employs adenoviral mediated gene

transfer—a well characterized and useful tool for the delivery of DNA

sequences both in vivo and in vitro. Once inside a mammalian cell, a

modified CMV promoter drives the expression of a hairpin siRNA for

RNAi studies. Using this kit, researchers can produce a replication-deficient adenovirus containing a hairpin siRNA template that targets their

gene of interest. It has been shown that adenoviral vectors are capable of

expressing functional siRNAs that enter the RNAi pathway and reduce

target gene expression in both tissue culture cells and animals (Xia 2002).