pcDNA6.2-GWEmGFP-miR negative

型号	载体名称	出品公司	载体用途
VGI0404	pcDNA6.2-GWEmGFP-miR negative	Invitrogen	Gateway系统

Comments for:

CMV promoter:

attB1 site:

EmGFP:

EmGFP forward sequencing primer site:

5’ miR flanking region:

miR-neg control:

3’ miR flanking region:

attB2 site （c）:

miRNA reverse sequencing primer site （c）:

TK polyadenylation signal:

f1 origin:

SV40 early promoter and origin:

EM7 promoter:

Blasticidin resistance gene:

SV40 polyadenylation signal:

pUC origin （c）:

Spectinomycin resistance gene （c）:

Spect ni omycin

5’ miR flanking

 region attB1 EmGFP attB2

pcDNATM 6.2-GW/EmGFP-miR-neg

control plasmid

5759 nucleotides

1 – 588

680 – 704

713 – 1432

1409 – 1428

1492 – 1518

1519 – 1578

1579 – 1623

1652 – 1676

1667 – 1686

1705 – 1976

2088 – 2516

2543 – 2851

2906 – 2972

2973 – 3371

3529 – 3659

3797 – 4470

4540 – 5550