Dianova公司为德国的病理免疫组化产品供应商,具备先进的抗体制备工艺及严格的质控系统,产品在上享有良好的声誉。
Dianova DIA-H09-L说明书
DIA-H09-L – Anti-IDH1 R132H (Hu) from Mouse (Clone: H09) – prediluted RTU – 8 ml
DIA-H09-L – 来自小鼠的抗IDH1 R132H(Hu)(克隆:H09) – 预先稀释的RTU – 8 ml
Art.-Nr.
DIA-H09-L
特异性
IDH1 R132H
物种反应性
人的
寄主物种
老鼠
同型
的IgG2a
克隆
H09
克隆性(单克隆/多克隆)
单克隆
应用
免疫组化(石蜡包埋切片)
免疫组化(IHC)
稀释
预稀释 – 即用型
格式
0.05%NaN3
即用型
预先稀释的抗体
2%BSA
产品系列/主题
肿瘤标志物/生物学
神经生物学
有可能的使用
仅供研究使用
温度 – 储存
2-8℃
搜索代码
IHC,FFPE,石蜡
DIAH09L
制造商/品牌
Dianova
福尔马林固定石蜡包埋的脑组织切片中人IDH1 R132H的免疫组织化学
A:IDH1突变特异性抗体克隆H09在间变性少突细胞瘤的肿瘤中心的强烈反应。
B:间变性星形细胞瘤的浸润区,通过抗体克隆H09特异性标记浸润性神经胶质瘤细胞。
C:用抗体克隆H09鉴定远离肿瘤中心的白质中的单个肿瘤细胞。
D:由少突神经胶质瘤浸润的皮质,具有抗体克隆H09对肿瘤细胞的特异性标记。
E:GFAP(胶质原纤维酸性蛋白,红色)和少突胶质细胞瘤浸润区的H09(棕色)的双重染色,证明肿瘤细胞的特异性标记但不是GFAP阳性反应性星形胶质细胞的特异性标记。
F:抗体克隆H09与IDH1 R132H突变的弥漫性星形细胞瘤(左)的强烈反应,但不与野生型肿瘤(右)反应。
(图片由德国海德堡大学海德堡大学神经病理学系/
德国癌症研究中心(DKFZ)临床合作单位神经病理学系医学博士Andreas von Deimling提供)
背景
抗体克隆 H09与福尔马林固定脑肿瘤组织切片中异柠檬酸脱氢酶1 (IDH1)R132H点突变特异性反应。IDH1密码子132的杂合点突变在世界卫生组织(WHO)II级和III级神经胶质瘤中很常见。IDH1 R132H突变发生在大约70%的星形细胞瘤和少突神经胶质瘤中。IDH1 R132H突变在特定脑肿瘤实体中的高频率和分布允许通过免疫组织化学对各种肿瘤的高度敏感和特异性区分,例如来自原发性胶质母细胞瘤的间变性星形细胞瘤或来自毛细胞星形细胞瘤或室管膜瘤的弥散性星形细胞瘤WHO II级。值得注意的是,从反应性神经胶质增生中识别具有IDH1突变的肿瘤的浸润边缘。
ATRX(dianova clone AX1)和IDH1R132H(dianova clone H09)的组合免疫组织化学
取代了分子检测。
用于诊断星形细胞瘤和少突神经胶质瘤的常规实用方法开始于针对IDH1 R132H和ATRX表达的进行IHC 。用ATRX和IDH1 R132H的初始IHC逐步分析分子参数,然后进行1p / 19q分析,然后通过IDH测序,显着减少了明确诊断所需的分子检测次数(Reuss等,2015)。
参考
- Capper D等。特异于IDH1 R132H突变的单克隆抗体。Acta Neuropathol。118(5):599-601,2009
- Capper D等。表征R132H突变特异性IDH1抗体在脑肿瘤中的结合。脑病理学。20(1):245-254,2010
- Preusser M等。诊断性神经病理学中的IDH测试:欧洲 – 中枢神经系统研究委员会邀请的评论和实用指南文章。Clinical Neuropathology,30(5):217-230,2011
- Van den Bent MJ等。实验室间比较IDH突变检测。J Neurooncol 112:173-178,2013
- Schumacher T等人。靶向突变体IDH1的疫苗诱导抗肿瘤免疫。Nature 2014,DOI:10.1038 / nature13387
- Reuss DE等人。ATRX和IDH1-R132H免疫组织化学,随后的拷贝数分析和IDH测序作为成人星形细胞瘤,少突神经胶质瘤和胶质母细胞瘤的“整合”诊断方法的基础。Acta Neuropathol。129(1):133-146,2015
- David NL等人。2016年世界卫生组织中枢神经系统肿瘤分类:摘要。Acta Neuropathol。,131:803-820,2016
数量:8毫升
通常的送货时间:1个工作日
482,00 € 加上增值税
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相关产品
DIA-H09 – 来自小鼠的抗IDH1 R132H(Hu)(克隆:H09) – 500μl
DIA-H09-M – 来自小鼠的抗IDH1 R132H(Hu)(克隆:H09) – 0.1 ml
DIA-AX1 – 来自小鼠的抗ATRX(Hu)(克隆:AX1) – 不对称。 - 500μl
SKU |
Product Name |
Quantity |
Price |
DIA-H09 |
Anti-IDH1 R132H (Hu) from Mouse (Clone: H09) |
500 µl |
482,00 € plus VAT
Free shipping inside Germany |
DIA-H09-L |
Anti-IDH1 R132H (Hu) from Mouse (Clone: H09) – prediluted RTU |
8 ml |
482,00 € plus VAT
Free shipping inside Germany |
DIA-H09-M |
Anti-IDH1 R132H (Hu) from Mouse (Clone: H09) |
0,1 ml |
142,00 € plus VAT |
Dianova2019年产品价格表
货号 |
品名 |
规格 |
价格 |
品牌 |
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200µg |
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DIA-100-M |
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20µg |
0 |
dianova |
DIA-120 |
Anti-Fibroblasten (CD90) (Hu) from Mouse (Clone: AS02) -FITC |
100test |
5360 |
dianova |
DIA-530-P05 |
Anti-p53 (Hu) from Mouse (Clone: CC53) |
0.5ml |
4780 |
dianova |
DIA-530-M |
Anti-p53 (Hu) from Mouse (Clone: CC53) |
0.1ml |
0 |
dianova |
DIA-670-P05 |
Anti-Ki-67/MIB1 (Hu) from Mouse (Clone: Ki-67P) CE-IVD |
0.5ml |
4780 |
dianova |
DIA-670-P1 |
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1ml |
6500 |
dianova |
DIA-670-M |
Anti-Ki-67/MIB1 (Hu) from Mouse (Clone: Ki-67P) CE-IVD |
0.1ml |
0 |
dianova |
DIA-700-P05 |
Anti-GFAP (Hu) from Mouse (Clone: IF3) CE-IVD |
0.5ml |
4780 |
dianova |
DIA-700-M |
Anti-GFAP (Hu) from Mouse (Clone: IF3) CE-IVD |
0.5ml |
0 |
dianova |
DIA-AX1 |
Anti-ATRX (Hu) from Mouse (Clone: AX1) |
0.5ml |
6760 |
dianova |
DIA-CAL-100 |
Anti-CALRETICULIN mutation (Hu) from Mouse (Clone CAL2) |
0.1ml |
5960 |
dianova |
DIA-CAL-250 |
Anti-CALRETICULIN mutation (Hu) from Mouse (Clone CAL2) |
0.25ml |
13960 |
dianova |
DIA-H09 |
Anti-IDH1 R132H (Hu) from Mouse (Clone: H09) CE-IVD |
0.5ml |
9640 |
dianova |
DIA-H09-M |
Anti-IDH1 R132H (Hu) from Mouse (Clone: H09) CE-IVD |
0.1ml |
2840 |
dianova |
DIA-H09-L |
Anti-IDH1 R132H (Hu) from Mouse (Clone: H09), prediluted, ready-to-use |
8ml |
9640 |
dianova |
DIA-W09 |
Anti-IDH1 (Hu) from Rat (Clone: W09) |
0.5ml |
4960 |
dianova |
DIA-TG1 |
Anti-TIGIT (Hu) from Mouse (Clone TG1) |
0.5ml |
20400 |
dianova |
DIA-TG1-M |
Anti-TIGIT (Hu) from Mouse (Clone TG1) |
0.1ml |
5720 |
dianova |
DIA-TC8 |
Anti-CD8 (Hu) from Mouse (Clone TC8 ) |
0.5ml |
9200 |
dianova |
DIA-P16-OD |
Anti-P16 (Hu) from Mouse (Clone JAP16) |
0.1ml |
7360 |
dianova |
DIA-303 |
Anti-CD3 (Ms, FFPE) from Rat (Clone: HH3E) |
0.5ml |
6900 |
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DIA-310 |
Anti-CD31 (Ms, FFPE) from Rat (Clone: SZ31) |
0.5ml |
8040 |
dianova |
DIA-404 |
Anti-CD4 (Ms, FFPE) from Rat (Clone GHH8) |
0.2ml |
6900 |
dianova |
DIA-808 |
Anti-CD8a (Ms, FFPE) from Rat (Clone GHH8) |
0.2ml |
6900 |
dianova |
DIA-60 |
Anti-FasL, soluble (Hu) from Mouse (Clone: 94) |
1ml |
4760 |
dianova |
DIA-900-100 |
Anti-HIS-Tag (affinity 3x 10‐10M (Biacore™) from Mouse (Clone: 13/45/31) |
100µg |
2960 |
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DIA-900-200 |
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200µg |
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DIA-900-FITC |
Anti-HIS Epitope-Tag (affinity 3x 10‐10M (Biacore™) from Mouse (Clone:13/45/31) -FITC |
1ml |
5960 |
dianova |
DIA-900-BIOT |
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100µg |
3960 |
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DIA-910-200-ABF |
Anti-HIS Epitope-Tag (affinity 3x 10‐10M (Biacore™) from Mouse (Clone:13/45/31) Acid/BSA-free |
200µg |
4960 |
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DIA.910-1MG-ABF |
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50pcs. |
1620 |
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5pcs. |
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100pcs. |
2440 |
dianova |
SCR-038447 |
Immunoselect Antifading Mounting Medium |
15ml |
960 |
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1180 |
dianova |
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Immunoselect Antifading Mounting Medium PI without hardener |
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1180 |
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DIA-0302-I |
p53-auto antibody ELISA-Kit (Human) CE-IVD |
96test |
10960 |
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