重组人MEGACD40L® 蛋白（可溶）

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产品编号 产品名称 产品规格 产品等级 产品价格
ALX-522-110-C010  MEGACD40L (soluble) (human), (recombinant)
重组人Mega CD40L（可溶） 		 10 μg
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  • 参考文献

重组人MEGACD40L® 蛋白（可溶）重组人MEGACD40L® 蛋白（可溶）

用于免疫反应中协同刺激激活的高活性、高纯度CD40L蛋白质

◆特点

●　高活性MEGACD40L® 低聚物模拟体内膜辅助CD40L聚合和激活而无需增强剂

●　高纯度，低内毒素水平

●　丰富的生产经验保证产品批次间一致性和可靠性

●　优化的配方以增强稳定性并提高活性

MEGACD40L是一种高活性蛋白，是两个三聚体CD40配体分子通过Adiponectin/ACRP30/AdipoQ的胶原域人工连接而成。在体内，这种蛋白质能有效的模拟CD40L的天然膜辅助聚合。可简单有效的替代 [CD40L+增强剂]组合（产品编号：ALX-850-064）。

◆产品规格

别名

  CD40L:ACRP30headless, CD154:ACRP30headless,

  TNFSF 5:ACRP30headless,  gp39:ACRP30headless

分子量

  ~38 kDa

来源

  CHO细胞表达。人CD40L（CD154）（aa 116-261）与小鼠ACRP30headless

  (aa 18-111)N端以及一个FLAG® -tag融合。

UniProt ID

  P29965 (human CD40L), Q60994 (mouse Adiponectin)

浓度

  复溶后0.1 mg/mL

产品类型

  冻干，含PBS

纯度

  ≥90%（SDS-PAGE）

纯化细节

  多步层析纯化

内毒素含量

  ≤0.01EU/μg 纯化蛋白（LAL 测试；Cape Cod Associates）

生物活性

  与人CD40结合。B细胞激活（已证明存在CD86剂量依赖性上调）

应用

  可用于调节免疫和炎症反应，作为免疫治疗的临床前研究的一种蛋白质工具，

  用来监测病人接受化疗的过程以及模拟自体免疫反应表型。

配制

  用100 μL的无菌水溶解。以含5%胎牛血清的培养基进行进一步稀释。

运输

  冰袋运输

长期储存

  -20℃

使用/稳定性

  收货后可稳定储存在-20℃至少6个月

处理

  避免反复冻融。复溶后，分装成小份储存于-20℃。

技术信息/产品说明

  FLAG是Sigma-Aldrich Co.的注册商标。

◆应用实例

重组人MEGACD40L® 蛋白（可溶）

MEGACD40L® (产品编号：ALX-522-110)激活B淋巴细胞。方法：外周血单核细胞（PBMCs）37℃、5%CO2 在48孔板（1×106 细胞/孔）中培养48小时。每孔包含200 μL已梯度稀释的MEGACD40L® 无血清测试培养基。处理后，清洗细胞，用小鼠anti-human CD19-PE和小鼠anti-humanCD86-APC进行双重染色，然后用流式细胞术进行分析。所示数据为在不同的MEGACD40L® 浓度下，共染色为CD19+ 和CD86+ B细胞的百分数。

重组人MEGACD40L® 蛋白（可溶）

重组人MEGACD40L®（可溶）（产品编号：ALX-522-110）结构示意图


重组人MEGACD40L® 蛋白（可溶）

PBMCs（外周血单核细胞）37℃、5%CO2 在48孔板（1×106细胞/孔）中培养48小时，每孔包含200 μL已梯度稀释的MEGACD40L®（产品编号：ALX-522-110）无血清测试培养基、CD40L(产品编号：ALX-522-015)和CD40L+2 μg/mL 增强剂（产品编号：ALX-804-034）。处理后，用PBS+1%BSA清洗细胞3次。用小鼠anti-human CD19（PE偶联）和小鼠anti-humanCD86（APC偶联）进行双重染色，4℃，2小时。用PBS+1%BSA清洗细胞3次，用PBS重悬。样品用BD Facs Calibur 流式细胞分析仪进行分析。所示数据为在不同的浓度下CD86+B细胞百分数/CD19+B细胞百分数。

重组人MEGACD40L® 蛋白（可溶）

1000 ng/mL、7个连续批次的MEGACD40L® 用于B细胞激活实验数据


重组人MEGACD40L® 蛋白（可溶）

通过在指定的温度下储存蛋白2个月来进行MEGACD40L® 稳定性测试。用B细胞激活实验来进行蛋白活性测试。结果显示在严苛的储存条件下MEGACD40L® 仍能保持较好的活性。

◆相关产品


产品编号

产品名称

包装

产品描述

ALX-522-120-C010

MEGACD40L (soluble) (mouse), (recombinant)
MegaCD40L （可溶性） （小鼠）, （重组）

10 μg

用于免疫反应中协同激活活性的高活性、高纯度CD40L蛋白。

ALX-850-064-KI01

CD40L, soluble (human) (recombinant) set
CD40L, 可溶性 （人） （重组） 套装

1 set

配体+增强剂用于提高稳定性和增强免疫活性。

ADI-CSA-187-E

CD 40L monoclonal antibody (5F3)
CD40L, 单抗 （5F3）

100 μg

流式细胞术

ALX-805-037-C100

CD40L (human) monoclonal antibody (blocking) (MK13A4)
CD40L （人）, 单抗 （封闭作用） （MK13A4）

100 μg

流式细胞术，FUNC

ALX-522-015-6010

CD40L (soluble) (human), (recombinant)
  CD40L （可溶性） （人）, （重组）

6×10 μg

增加对B细胞的刺激。
由E.coli表达。人CD40L（CD154）（aa 116-261）胞外区域与一个短肽（6   aa）的N端以及一个FLAG®-tag融合，≥95%（SDS-PAGE），ELISA

ALX-522-015-2010

2×10 μg

ALX-522-015-C010

10 μg

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