| 组织特异性cDNA(大鼠) | ||||||
| 品牌 | Code No. | 产品名称 | 包装量 | 价格（元） | 说明书 | 数量 |
| Clontech | 637312 | Rat Brain QUICK-Clone cDNA | 2 × 10 Rxns | ￥7,931 |  |
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| Clontech | 637314 | Rat Heart QUICK-Clone cDNA | 2 × 10 Rxns | ￥7,931 | |
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| Clontech | 637317 | Rat Kidney QUICK-Clone cDNA | 2 × 10 Rxns | ￥7,931 | |
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| Clontech | 637313 | Rat Liver QUICK-Clone cDNA | 2 × 10 Rxns | ￥7,931 | |
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| Clontech | 637318 | Rat Pancreas QUICK-Clone cDNA | 2 × 10 Rxns | ￥7,931 | |
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| Clontech | 637315 | Rat Spleen QUICK-Clone cDNA | 2 × 10 Rxns | ￥7,931 | |
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| Clontech | 637316 | Rat Testis QUICK-Clone cDNA | 2 × 10 Rxns | ￥7,931 | |
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| 高质量的cDNA模板是PCR扩增得到良好结果的必备条件。QUICK-Clone cDNA是预制的双链cDNA，您可以使用基因特异性引物从中扩增感兴趣的序列，是扩增先前分离的、结构相关的或跨物种cDNA的理想选择。使用oligo (dT) 引物从来自多种大鼠组织的高质量poly A+ RNA合成的QUICK-Clone cDNA，经纯化以去除残留的RNA并去除小于400 bp的cDNA片段。使用QUICK-Clone cDNA可让您扩增感兴趣的cDNA，同时避免传统的文库构建和筛选步骤，还可用于使用基因特异性或简并引物制作杂交探针 (Parmentier et al. 1989; Wilks et al. 1989; Vallins et al. 1990; Lee et al. 1988; Schuchman, Jackson, and Desnick 1990)。 |
| 概述 |
| · 高纯度的双链cDNA · 直接通过PCR克隆基因，而不是文库筛选 · 从高质量的大鼠组织和细胞系中制备 · 非常适合扩增先前分离的、结构相关的或跨物种的cDNA
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| 更多信息 |
| 应用 |
| · 无需文库筛选即可克隆cDNA · 使用基因特异性或简并引物制作杂交探针 · 非常适合扩增先前分离的、结构相关的或跨物种的cDNA |
| 参考文献 |
| Lee, C. et al. Generation of cDNA probes directed by amino acid sequence: cloning of urate oxidase. Science 239 (1988). |
| Parmentier, M. et al. Molecular cloning of the thyrotropin receptor. Science 246 (1989). |
| Schuchman, E. H., Jackson, C. E. & Desnick, R. J. Human arylsulfatase B: MOPAC cloning, nucleotide sequence of a full-length cDNA, and regions of amino acid identity with arylsulfatases A and C. Genomics 6, 149–158 (1990). |
| Vallins, W. J. et al. Molecular cloning of human cardiac troponin I using polymerase chain reaction. FEBS Lett. 270, 57–61 (1990). |
| Wilks, A. F., Kurban, R. R., Hovens, C. M. & Ralph, S. J. The application of the polymerase chain reaction to cloning members of the protein tyrosine kinase family. Gene 85, 67-74 (1989). |
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