фX174 Virion DNA | NEB酶试剂 New England Biolabs

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ΦX174 Virion DNA

  • View sequence details
  • ΦX174 Virion DNA is the single-stranded viral DNA isolated from bacteriophage ΦX174 am3 cs70.
  • Molecular weight: 1.70 x 106 daltons
  • Size: 5386 bases

фX174 Virion DNA | NEB酶试剂 New England Biolabs

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
N3023S 1,000 µg/ml 50 µg ¥899.00 有 无 有 无 无
N3023L 1,000 µg/ml 250 µg ¥3,419.00 有 无 无 无 无
如何订购
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产品信息

ΦX174 Virion DNA is the single-stranded viral DNA isolated from bacteriophage ΦX174 am3 cs70. It has a molecular weight of 1.70 x 106 daltons and is 5386 bases in length. Greater than 85% of the molecules are circular.

DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.

产品来源

The phage is grown in E. coli HF4704 and purified by cesium chloride density gradient centrifugation. The DNA is isolated from the purified phage by phenol extraction according to the procedure of Sinsheimer.

产品类别:
DNA Plasmids & Substrates Products

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • N3023S     -20    
        ΦX174 Virion DNA N3023SVIAL -20 1 x 0.05 ml 1 mg/ml
    • N3023L     -20    
        ΦX174 Virion DNA N3023LVIAL -20 1 x 0.25 ml 1 mg/ml

  • 特性和用法

    贮存溶液

    10 mM Tris-HCl
    1 mM EDTA
    pH 8 @ 25°C

  • 相关产品

    相关产品

    • фX174 RF I DNA
    • фX174 RF II DNA
    • Monarch® Plasmid Miniprep Kit 
    • Monarch® DNA Gel Extraction Kit
    • Monarch® PCR & DNA Cleanup Kit (5 μg)

  • 注意事项
    1. ϕhiX174 is available in different forms: single-stranded (from phage particles) or double-stranded (from phage infected E.coli). Generally, single-stranded DNAs are not cut by restriction enzymes.

  • 参考文献
    1. Sanger, F., Coulson, A.R., Friedmann, T., Air, G.M., Barrell, B.G., Brown, N.L., Fiddes, J. C., Hutchison, C.A. III, Slocombe, P.M. and Smith, M. (1978). J. Mol.Biol.. 125, 225-246.
    2. Sinsheimer, R.L. (1966). Cantoni and Davies(Ed.), Procedures in Nucleic Acid Research. 569. Harper and Row.

工具 & 资源

  • Web 工具
    • DNA Sequences and Maps Tool

FAQs & 问题解决指南

  • FAQs
    1. What is the DNA sequence of this product?

  • 实验技巧
    φhiX174 is available in different forms: single-stranded (from phage particles) or double-stranded (from phage infected E.coli). Generally, single-stranded DNAs are not cut by restriction enzymes.

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    фX174 Virion DNA | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

质控、安全 & 法规

  • 质控分析
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  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • N3023S_L_v1

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • N3023S_L_v1_1241407
    • N3023S_L_v1_1251704
    • N3023S_L_v1_1261712
    • N3023L_v1_10014944
    • N3023S_v1_10014946
    • N3023L_v1_10041287
    • N3023S_v1_10041289
    • N3023L_v1_10062072
    • N3023S_v1_10062073
    • N3023L_v1_10070480
    • N3023S_v1_10070482
    • N3023L_v1_10081344
    • N3023S_v1_10091083
    • N3023L_v1_10092839
    • N3023S_v1_10092841
    • N3023L_v1_10105326
    • N3023S_v1_10110285
    • N3023L_v1_10126536
    • N3023S_v1_10126538
    • N3023L_v1_10139913
    • N3023L_v1_10147355
    • N3023S_v1_10150026

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • ΦX174 Virion DNA