M13mp18 Single-stranded DNA | NEB酶试剂 New England Biolabs

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M13mp18 Single-stranded DNA

  • View sequence details
  • Single stranded, circular viral DNA from M13mp18
  • Useful as a template in the dideoxy-nucleotide termination sequencing method

M13mp18 Single-stranded DNA | NEB酶试剂 New England Biolabs

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N4040S 250 µg/ml 10 µg ¥519.00 有 有 有 无 有
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产品信息

The single-stranded viral DNA is isolated from M13mp18. M13mp18 is a M13 lac phage vector which contains single HindIII, SphI, SbfI, PstI, SalI (AccI/ HincII), XbaI, BamHI, SmaI (XmaI), KpnI (Acc65I), SacI and EcoRI sites within the gene encoding β-Galactosidase.This DNA preparation is useful as a standard and has been tested as a template in the dideoxy-nucleotide termination method of sequencing DNA.

来源

M13mp18 phage is propagated in E. coli ER2738.The phage is purified by polyethylene glycol precipitation. The single-strandedDNA is then extracted with phenol.

产品类别:
DNA Plasmids & Substrates Products

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • N4040S     -20    
        M13mp18 Single-stranded DNA N4040SVIAL -20 1 x 0.04 ml 250 µg/ml

  • 特性和用法

    贮存溶液

    10 mM Tris-HCl
    1 mM EDTA
    pH 8 @ 25°C

  • 相关产品

    相关产品

    • M13mp18 RF I DNA
    • Monarch® Plasmid Miniprep Kit 
    • Monarch® DNA Gel Extraction Kit
    • Monarch® PCR & DNA Cleanup Kit (5 μg)

  • 注意事项
    1. M13mp18 DNA may occur in two different forms:
      1. Single-stranded (from phage particles); M13mp18 Single-Stranded DNA or
      2. Double-stranded (from phage infected E.coli); M13mp18 RFI DNA.

      Generally single-stranded DNAs are not cut by restriction enzymes.

  • 参考文献
    1. Messing, J. (1983). R. Wu(Ed.), Methods Enzymol.. 101, 20. San Diego: Academic Press.

操作说明、说明书 & 用法

  • 操作说明
    1. M13mp18 Phage Transfection

FAQs & 问题解决指南

  • FAQs
    1. Is the M13mp18 Single-Stranded DNA (N4040S) circular or linear?
    2. M13mp18 DNA may occur in two different forms:
    3. What is the DNA sequence of this product?

  • 实验技巧

    M13mp18 DNA may occur in two different forms:

    1. Single-stranded (from phage particles); M13mp18 Single-Stranded DNA or
    2. Double-stranded (from phage infected E.coli); M13mp18 RFI DNA.

    Generally single-stranded DNAs are not cut by restriction enzymes.

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

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质控、安全 & 法规

  • 质控分析
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  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • N4040S_v2

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • N4040S_v2_0161401
    • N4040S_v2_0161407
    • N4040S_v2_0161412
    • N4040S_v1_0161505
    • N4040S_v2_0161505
    • N4040S_v2_0161509
    • N4040S_v2_0161511
    • N4040S_v2_0161604
    • N4040S_v2_0161608
    • N4040S_v2_0161612
    • N4040S_v2_0181704
    • N4040S_v2_0181711
    • N4040S_v2_10012712
    • N4040S_v2_10015742
    • N4040S_v2_10019261
    • N4040S_v2_10021428
    • N4040S_v2_10026363
    • N4040S_v2_10034840
    • N4040S_v2_10042806
    • N4040S_v2_10046526
    • N4040S_v2_10052522
    • N4040S_v2_10059943
    • N4040S_v2_10063189
    • N4040S_v2_10065784
    • N4040S_v2_10068067
    • N4040S_v2_10077569
    • N4040S_v2_10080165
    • N4040S_v2_10082621
    • N4040S_v2_10085103
    • N4040S_v2_10092186
    • N4040S_v2_10099228
    • N4040S_v2_10122684
    • N4040S_v2_10128627
    • N4040S_v2_10111163
    • N4040S_v2_10118171
    • N4040S_v2_10139437
    • N4040S_v2_10142677
    • N4040S_v2_10150978
    • N4040S_v2_10160098

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • M13mp18 Single-stranded DNA