M13mp18 RF I DNA | NEB酶试剂 New England Biolabs

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M13mp18 RF I DNA

  • View sequence details
  • Phage vectors derived from bacteriophage M13
  • Covalently closed circular DNA
  • Includes 13 unique restriction sites, and β-galactosidase gene
  • Blue/white selection

M13mp18 RF I DNA | NEB酶试剂 New England Biolabs

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
N4018S 100 µg/ml 10 µg ¥909.00 有 有 有 无 无
如何订购
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产品信息

M13mp18 is the double-stranded, covalently closed, circular form of DNA derived from bacteriophage M13. This phage vector contains single HindIII, SphI, SbfI, PstI, SalI (AccI/ HincII), XbaI, BamHI, SmaI (XmaI), KpnI (Acc65I), SacI and EcoRI sites within the β-Galactosidase gene (1). When a fragment of DNA is inserted into one of these sites, the β-Galactosidase gene is inactivated, providing selection for clones on the appropriate indicator plate (2).

DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.

产品来源

The phage M13mp18 is propagated in E. coli ER2738(3). The replicative form of DNA is isolated from infected cells and purified by a standard plasmid purification procedure. The final preparation is tested for its suitability as a vector.

产品类别:
DNA Plasmids & Substrates Products

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • N4018S     -20    
        M13mp18 RF I DNA N4018SVIAL -20 1 x 0.1 ml 100 µg/ml

  • 特性和用法

    贮存溶液

    10 mM Tris-HCl
    1 mM EDTA
    pH 8 @ 25°C

  • 相关产品

    相关产品

    • M13mp18 Single-stranded DNA
    • Monarch® Plasmid Miniprep Kit 
    • Monarch® DNA Gel Extraction Kit
    • Monarch® PCR & DNA Cleanup Kit (5 μg)

  • 注意事项
    1. M13mp18 DNA may occur in two different forms:
      1. Single-stranded (from phage particles); M13mp18 Single-Stranded DNA or
      2. Double-stranded (from phage infected E.coli); M13mp18 RFI DNA.

      Generally single-stranded DNAs are not cut by restriction enzymes.

  • 参考文献
    1. Norrander, J., Kempe, T. and Messing, J. (1983). Gene. 26, 101-106.
    2. Messing, J., Crea, R. and Seeburg, P.H. (1981). Nucl. Acids Res.. 9, 309-321.
    3. Messing, J. (1979). Recombinant DNA Technical Bulletin (NIH). 2, 43-48.

操作说明、说明书 & 用法

  • 操作说明
    1. M13mp18 Phage Transfection

工具 & 资源

  • Web 工具
    • DNA Sequences and Maps Tool

FAQs & 问题解决指南

  • FAQs
    1. What is the DNA sequence of this product?

  • 实验技巧

    M13mp18 DNA may occur in two different forms:

    1. Single-stranded (from phage particles); M13mp18 Single-Stranded DNA or
    2. Double-stranded (from phage infected E.coli); M13mp18 RFI DNA.

    Generally single-stranded DNAs are not cut by restriction enzymes.

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    M13mp18 RF I DNA | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

质控、安全 & 法规

  • 质控分析
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  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • N4018S_v1

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • N4018S_v1_0421401
    • N4018S_v1_0421407
    • N4018S_v1_0421504
    • N4018S_v1_0421511
    • N4018S_v1_0421604
    • N4018S_v1_0421608
    • N4018S_v1_0421612
    • N4018S_v1_0421704
    • N4018S_v1_0421709
    • N4018S_v1_10011801
    • N4018S_v1_10033484
    • N4018S_v1_10047725
    • N4018S_v1_10059897
    • N4018S_v1_10078107
    • N4018S_v1_10086916
    • N4018S_v1_10100841
    • N4018S_v1_10118740
    • N4018S_v1_10139509
    • N4018S_v1_10151998

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • M13mp18 RF I DNA