polyA Spin™ mRNA Isolation Kit | NEB酶试剂 New England Biolabs

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polyA Spin™ mRNA Isolation Kit

We recommend Oligo d(T)25 Magnetic Beads (NEB #S1419S) as an alternative for purification of mRNA containing polyadenylic (poly A) regions. For NGS applications, the NEBNext Poly(A) mRNA Isolation Module (NEB #E7490) is recommended.

  • Catalog # S1560 was discontinued on March 31, 2020

This kit contains an affinity matrix used for the isolation of mRNA containing polyadenylic (poly A) regions (1) from purified total RNA. This matrix consists of oligo (dT)25 covalently coupled to a cross-linked cellulose bead. This product is guaranteed for twenty-four months when stored at 4°C in the supplied storage buffer.

  • A rapid and convenient alternative to traditional column chromatography for the isolation of full-length poly(A)+ eukaryotic messenger (mRNA) from samples of Total RNA
  • Shorter purification time: Prepare full-length mRNA in as little as 40 minutes
  • Maintains Resolution: When compared to a 100 mg column of high quality oligo (dT) cellulose in 1.0 mg total RNA sample range

Storage Temperature:  4°C

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polyA Spin™ mRNA Isolation Kit | NEB酶试剂 New England Biolabs

Avoiding RNase Contamination

产品信息

The polyA Spin™ Kit is a rapid and convenient alternative to traditional column chromatography for the isolation of full-length poly(A)+ eukaryotic messenger (mRNA) from samples of “total” RNA. Poly(A)+ RNA selection is made by affinity chromatography using spin columns prepackaged with NEB’s Oligo (dT)25-Cellulose beads (NEB #S1408S). Prepared essentially by the method of Gilham (1), this solid support has found widespread use in the “batch” preparation of low abundance mRNA (2). Its high binding capacity and rapid hybridization kinetics make it an ideal support for chromatographic poly(A)+ RNA selection. Intact mRNA can be isolated in as little as forty minutes from multiple cell lysates or samples of “total” RNA by spin chromatography in a microcentrifuge.
Reagents sufficient for the isolation and subsequent precipitation of poly(A)+ RNA from eight samples of as much as 1.0 mg of “total” RNA are provided. The isolated RNA can be used for in vitro translation experiments, the preparation of cDNA libraries, Northern analysis, subtractive hybridization or differential display.

产品类别:
Discontinued Products

  • 特性和用法

  • 优势和特性

    Features

    • Shorter Purification Time
    • Reduced Isolation Volumes
    • Maintains Resolution: When compared to a 100 mg column of high quality oligo (dT) cellulose in 1.0 mg total RNA sample range.

  • 注意事项
    1. The polyA Spin mRNA Isolation Kit complements and serves as an alternative to traditional column chromatography isolation of mRNA. Each polyA Spin spin column has the same column performance as a standard 100 mg column of high quality oligo (dT)-cellulose in the 1.0 mg total RNA sample range. Thus the shorter purification times and reduced working volumes associated with spin chromatography are realized while maintaining column chromatography levels of performance. This is of particular importance in the isolation of low abundance messenger RNA (1). For total RNA samples greater than 1.0 mg initial “first round” isolation of poly(A)+ material should be done by column or “batch” (NEB #S1408S) chromatography (2). First round isolation of poly(A)+ RNA by either column or spin chromatography typically yields a product contaminated with poly(A)– RNA. PolyA Spin spin chromatography is ideal for “second round” purification, yielding material with an A260/280 of 1.9 or greater with minimal product loss. The protocol has been optimized for the isolation of poly(A)+ RNA from 0.1–1.0 mg of total RNA isolated from eukaryotic tissue or cells that contain between 1–5% mRNA. The amount of poly(A)+ RNA isolated will vary with the type of tissue or cells used.
    2. This product is not designed to work with in-vitro RNA.

  • 参考文献
    1. Gilham, P.T. (1964). J. Amer. Chem. Soc.. 86, 4982.
    2. Farrell, R.E. (1993). RNA Methodologies. 104-106.
    3. Jacobson, A. (1987). S. Berger and A. Kimmel(Ed.), Methods Enzymol.. 152, 254-257. San Diego: Academic Press.

操作说明、说明书 & 用法

  • 操作说明
    1. Isolation of mRNA Using the PolyA Spin™ mRNA Isolation Kit
    2. Batch Method Protocol (S1560) for Isolation of mRNA Using the PolyA Spin™ mRNA Isolation Kit

  • 说明书
    产品说明书包含产品使用的详细信息、产品配方和质控分析。

    • manualS1560

  • 使用指南
    • cDNA/Reverse Transcriptase Tips

FAQs & 问题解决指南

  • 实验技巧
    Do not freeze. To preserve its components’ integrity the kit has to be stored between 2 and 8 degrees.
    The denaturing step is critical for adequate mRNA recovery so please, make sure your sample is thoroughly denatured before incubating it with the beads.
    Centrifugation speed should never exceed 12,000 x g

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  • 引用文献

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质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • S1560S_v1

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • S1560S_v0_10009227
    • S1560S_v0_10020511
    • S1560S_v1_10033588
    • S1560S_v1_10046561
    • S1560S_v1_10051851