NEB酶试剂 New England Biolabsuffer Set 1.1, 2.1, 3.1 and CutSmart | NEB酶试剂 New England Biolabs

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NEBuffer™ Set (1.1, 2.1, 3.1 and CutSmart®)

This product has been replaced with NEBuffer™ Set (r1.1, r2.1, r3.1 and rCutSmart™) (NEB #B7030).

  • Catalog # B7200 was discontinued on December 15, 2021
New England Biolabs provides 10X NEBuffer with each restriction endonuclease to assure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. 

Set contains 1.25ml vial of each buffer.

 

精选视频

NEB酶试剂 New England Biolabsuffer Set 1.1, 2.1, 3.1 and CutSmart | NEB酶试剂 New England Biolabs

Traditional Cloning Workflow

产品信息

New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer.

 

NEBuffer 1.1
1X Buffer Components
10mM Bis-Tris-Propane-HCl
10mM MgCl2
100μg/ml BSA
pH [email protected]°C

NEBuffer 2.1
1X Buffer Components
50mM NaCl
10mM Tris-HCl
10mM MgCl2
100μg/ml BSA
pH [email protected]°C

NEBuffer 3.1
1X Buffer Components
100mM NaCl
50mM Tris-HCl
10mM MgCl2
100μg/ml BSA
pH [email protected]°C

CutSmart Buffer
1X Buffer Components
50mM Potassium Acetate
20mM Tris-acetate
10mM Magnesium Acetate
100μg/ml BSA
pH [email protected]°C

产品类别:
Discontinued Products

  • 特性和用法

  • 相关产品

    相关产品

    • Monarch® Plasmid Miniprep Kit 
    • Monarch® DNA Gel Extraction Kit
    • Monarch® PCR & DNA Cleanup Kit (5 μg)

  • 注意事项
    1. pH range: The pH of 10X NEBuffer 1.1 is between pH 6.9 and 7.1. The pH of 10X NEBuffer 2.1, NEBuffer 3.1 and CutSmart Buffer is between pH 7.8 and 8.0.

FAQs & 问题解决指南

  • FAQs
    1. Why do I see additional DNA bands on my gel after a restriction digest?
    2. Why is my Restriction Enzyme not cutting DNA?
    3. Why do I see a DNA smear on an agarose gel after a restriction digest?
    4. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    NEB酶试剂 New England Biolabsuffer Set 1.1, 2.1, 3.1 and CutSmart | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • B7200S_v1

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • B7200S_v0_10045728
    • B7200S_v1_10047928
    • B7200S_v1_10053286
    • B7200S_v1_10059052
    • B7200S_v1_10065823
    • B7200S_v1_10067914
    • B7200S_v1_10077456
    • B7200S_v1_10090689
    • B7200S_v1_10092812
    • B7200S_v1_10095451
    • B7200S_v1_10096608
    • B7200S_v1_10097619
    • B7200S_v1_10099639