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HeLa Genomic DNA
- Catalog # N4006 was discontinued on December 15, 2021
- Highly pure HeLa Genomic DNA for use in a wide variety of applications including: PCR, SNP analysis and southern blotting
- Genomic DNA library construction
- Control DNA for Methylation-specific PCR (MSP), Bisulfite sequencing, Methylation-sensitive Single-Nucleotide Primer Extension (MS-SNuPE,) Combined Bisulfite Restriction Analysis (COBRA), Bisulfite treatment and PCR-single strand Confirmation Polymorphism Analysis (Bisulfite-PCR-SSCP/BiPS)
产品信息
Product Source:
HeLa (cervix adenocarcinoma) cells were grown to confluency in DMEM plus 10% fetal bovine serum. Genomic DNA was isolated by a standard genomic purification protocol (1), phenol extracted and equilibrated to 10 mM TrisHCl (pH 7.5) and 1 mM EDTA.
- 产品类别:
- Discontinued Products
- 特性和用法
贮存溶液
10 mM Tris-HCl
1 mM EDTA
pH 7.5 @ 25°C - 优势和特性
应用特性
- A control for Methylation-Specific PCR (MSP) (2), Bisulfite sequencing, Methylation-sensitive Single-Nucleotide Primer Extension (Ms-SNuPE), Combined Bisulfite Restriciton Analysis (COBRA), Bisulfite treatment and PCR-Single-Strand Conformation Polymorphism Analysis (Bisulfite-PCR-SSCP/BiPS).
- PCR, SNP Analysis, Southern Blotting
- Genomic DNA library construction
- 注意事项
- Avoid multiple freeze/thaw cycles.
- 参考文献
- Sambrook, J. and Russell, D. (2001). Molecular Cloning: A Laboratory Manual. (3rd ed.), (pp. 6.4-6.12). Cold Spring Harbor: Cold Spring Ha.
引用 & 技术文献
- 引用文献
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质控、安全 & 法规
- 质控分析
每一新批次的 NEB 产品都要经过质控,以满足指定的质量标准,对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息,可从此处查阅。
- 产品说明与变更通知
产品说明与变更通知
产品说明表包含产品的储存温度、有效期和规格,这些文件的命名规则如下:[货号]_[规格]_[版本]
- N4006S_v1
- CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控,这些文件的命名规则如下: [货号]_[规格]_[版本]_[Lot#]
- N4006S_v1_0051603
- N4006S_v1_0051608
- N4006S_v1_0051612
- N4006S_v1_0051704
- N4006S_v1_0051708
- N4006S_v1_0051711
- N4006S_v1_10009459
- N4006S_v1_10026020
- N4006S_v1_10036630
- N4006S_v1_10058101
- N4006S_v1_10073960
- N4006S_v1_10077859
- N4006S_v1_10097031
- N4006S_v1_10116296
- SDS
以下 SDS 文件可以帮助您安全地使用该产品
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HeLa Genomic DNA
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