EagI |NEB酶试剂 New England Biolabs

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EagI

This product has been discontinued and is replaced with the High-Fidelity version, EagI-HF (NEB #R3505)

  • Catalog # R0505 was discontinued on March 17, 2021
EagI |
同裂酶 | 单字母标示 | Pronunciation: EagI |
  • Time-Saver™ qualified for digestion in 5-15 minutes
  • High Fidelity (HF®) version available (NEB #3505) supplied with CutSmart® Buffer
  • Supplied with 1 vial of Gel Loading Dye, Purple (6X)
  • Restriction Enzyme Cut Site: C/GGCCG

精选视频

EagI |

Reduce Star Activity with High-Fidelity Restriction Enzymes

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产品信息

EagI has a High Fidelity version, EagI-HF® (NEB #R3505)

High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

产品来源

An E. coli strain that carries the EagI gene from Enterobacter agglomerans (R. Morgan).

产品类别:
Discontinued Products

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme required to digest 1 µg of pXba DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

    反应条件

    1X NEBuffer™ 3.1
    Incubate at 37°C

    1X NEBuffer™ 3.1
    100 mM NaCl
    50 mM Tris-HCl
    10 mM MgCl2
    100 µg/ml BSA
    (pH 7.9 @ 25°C)

    在不同缓冲液中的活性

    NEBuffer™ 1.1: 10%
    NEBuffer™ 2.1: 25%
    NEBuffer™ 3.1: 100%

    稀释兼容性

    • 稀释液 B

    贮存溶液

    10 mM Tris-HCl
    500 mM NaCl
    1 mM DTT
    0.1 mM EDTA
    200 µg/ml BSA
    50% Glycerol
    pH 8 @ 25°C

    热失活

    65°C for 20 min

    甲基化敏感性

    dam 甲基化: Not Sensitive
    dcm 甲基化: Not Sensitive
    CpG甲基化: Blocked

    同裂酶

    XmaIII+

  • 相关产品

    相关产品

    • EagI-HF®
    • Monarch® Plasmid Miniprep Kit 
    • Monarch® DNA Gel Extraction Kit
    • Monarch® PCR & DNA Cleanup Kit (5 μg)

    单独销售的组分

    • Gel Loading Dye, Purple (6X)
    • NEBuffer 3.1

  • 注意事项
    1. EagI is an isoschizomer of XmaIII.
    2. For full EagI activity, the pH of the reaction mix must be between (7.9 and 9.0 @ 25°C).
    3. This enzyme has shown to have lower activity on some supercoiled plasmids, with more than 1 unit required to digest 1 μg plasmid DNA. For complete digestion of 1 μg of plasmid DNA please follow our recommended digestion protocol.
    4. Blocked by CpG methylation.

操作说明、说明书 & 用法

  • 操作说明
    1. Optimizing Restriction Endonuclease Reactions
    2. Restriction Digest Protocol
    3. Double Digest Protocol with Standard Restriction Enzymes

  • 使用指南
    • Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
    • Activity of Restriction Enzymes in PCR Buffers
    • Cleavage Close to the End of DNA Fragments
    • Dam and Dcm Methylases of E. coli
    • Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
    • Double Digests
    • Effects of CpG Methylation on Restriction Enzyme Cleavage
    • Heat Inactivation
    • NEBuffer Activity/Performance Chart with Restriction Enzymes
    • Optimizing Restriction Endonuclease Reactions
    • Restriction Endonucleases – Survival in a Reaction
    • Restriction Enzyme Diluent Buffer Compatibility
    • Restriction Enzyme Tips
    • Single Letter Codes
    • Star Activity
    • Traditional Cloning Quick Guide

工具 & 资源

  • 选择指南
    • Alphabetized List of Recognition Sequences
    • Cleavage of Supercoiled DNA
    • Compatible Cohesive Ends and Generation of New Restriction Sites
    • Dam-Dcm and CpG Methylation
    • Frequencies of Restriction Sites
    • Isoelectric Points (pI) for Restriction Enzymes
    • Isoschizomers
    • NEB Diluent and Buffer Table
    • Recleavable Filled-in 5′ Overhangs
    • Time-Saver™ Qualified Enzymes
    • Why Choose Recombinant Enzymes?

  • Web 工具
    • Competitor Cross-Reference Tool
    • DNA Sequences and Maps Tool
    • Double Digest Finder
    • Enzyme Finder
    • NEBcutter™ v3.0
    • NEBioCalculator®
    • REBASE®

FAQs & 问题解决指南

  • FAQs
    1. Does EagI produce commonly used compatible ends?
    2. Are more units of EagI required to cut supercoiled DNA than lambda DNA?
    3. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
    4. Why is my Restriction Enzyme not cutting DNA?
    5. Why do I see a DNA smear on an agarose gel after a restriction digest?
    6. Why do I see additional DNA bands on my gel after a restriction digest?
    7. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
    8. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
    9. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?

  • 问题解决指南
    • Restriction Enzyme Troubleshooting Guide

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    EagI | Powered by Bioz See more details on Bioz

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • R0505M_v1
    • R0505S_L_v1

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • R0505S_L_v1_0511401
    • R0505M_v1_0471303
    • R0505M_v1_0491307
    • R0505S_L_v1_0471303
    • R0505S_L_v1_0491308
    • R0505S_L_v1_0521410
    • R0505S_L_v1_0511410
    • R0505M_v1_0521411
    • R0505S_L_v1_0521411
    • R0505S_L_v1_0541505
    • R0505S_L_v1_0521407
    • R0505M_v1_0511402
    • R0505M_v1_0531501
    • R0505M_v1_0531505
    • R0505S_L_v1_0551602
    • R0505S_L_v1_0551605
    • R0505M_v1_0551602
    • R0505M_v1_0551608
    • R0505S_L_v1_0571611
    • R0505M_v1_0571709
    • R0505S_L_v1_0571705
    • R0505S_L_v1_0571712
    • R0505M_v1_0571803
    • R0505L_v1_10008867
    • R0505S_v1_10010521
    • R0505M_v1_10021733
    • R0505L_v1_10035405
    • R0505M_v1_10035407
    • R0505S_v1_10035409
    • R0505S_v1_10043635
    • R0505L_v1_10043637
    • R0505M_v1_10050279
    • R0505L_v1_10065277
    • R0505S_v1_10062746
    • R0505M_v1_10069598
    • R0505M_v1_10077743
    • R0505L_v1_10084611
    • R0505S_v1_10084612
    • R0505L_v1_10091754
    • R0505M_v1_10091766
    • R0505M_v1_10092489
    • R0505L_v1_10098734

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • EagI