CutSmart® Buffer | NEB酶试剂 New England Biolabs

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CutSmart® Buffer

This product has been replaced by rCutSmart Buffer (NEB #B6004).

  • Catalog # B7204 was discontinued on December 15, 2021
  • Over 210 enzymes are 100% active in CutSmart® Buffer simplifying double digests
  • Visit NEBCutSmart.com to learn more about the CutSmart Buffer system

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CutSmart® Buffer | NEB酶试剂 New England Biolabs

Traditional Cloning Workflow

产品信息

New England Biolabs provides a colorcoded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer.

Over 215 restriction enzymes are 100% active in CutSmart Buffer, making it significantly easier to set up your double digest reactions. Since CutSmart Buffer includes BSA, there are also fewer tubes and pipetting steps to worry about. Additionally, many DNA modifying enzymes are 100% active in CutSmart Buffer, eliminating the need for subsequent purification.

产品类别:
Discontinued Products

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • B7204V     -20    
        CutSmart® Buffer B7204SVIAL -20 1 x 1.25 ml 10 X

  • 特性和用法

    1X 缓冲液组分

    50 mM Potassium Acetate
    20 mM Tris-acetate
    10 mM Magnesium Acetate
    100 µg/ml BSA
    pH [email protected]°C

  • 相关产品

    相关产品

    • Monarch® Plasmid Miniprep Kit 
    • Monarch® DNA Gel Extraction Kit
    • Monarch® PCR & DNA Cleanup Kit (5 μg)

  • 注意事项
    1. The pH of 10X CutSmart Buffer is between pH 7.8 and 8.0.

操作说明、说明书 & 用法

  • 使用指南
    • Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

工具 & 资源

  • 选择指南
    • NEB Diluent and Buffer Table

FAQs & 问题解决指南

  • FAQs
    1. How should the NEBuffer be used?
    2. The buffer arrived thawed. Are the buffer and enzyme still active?
    3. You have replaced NEBuffer 1 with NEbuffer 1.1, NEBuffer 2 with NEBuffer 2.1, and NEBuffer 3 with 3.1. Where is NEBuffer 4.1?
    4. Why did you add BSA into all the restriction enzyme reaction buffers?
    5. Why did you remove DTT from your restriction enzyme buffers?
    6. I don’t want to use BSA in my buffer. What are my options? 
    7. Which restriction enzymes are used in GoldenBraid Assembly?
    8. Why do I see a DNA smear on an agarose gel after a restriction digest?
    9. Why is my Restriction Enzyme not cutting DNA?
    10. Why do I see additional DNA bands on my gel after a restriction digest?
    11. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
    12. Are the alkaline phosphatases active in NEBuffers?

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    CutSmart® Buffer | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • B7204S_v1

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • B7204S_v1_2971802
    • B7204S_v1_2981802
    • B7204S_v1_2991802
    • B7204S_v1_3001802
    • B7204S_v1_3011802
    • B7204S_v1_3031803
    • B7204S_v1_3041803
    • B7204S_v1_2941801
    • B7204S_v1_2951801
    • B7204S_v1_3051803
    • B7204S_v1_3061804
    • B7204S_v1_3071804
    • B7204S_v1_10012579
    • B7204S_v1_10022775
    • B7204S_v1_10026516
    • B7204S_v1_10030519
    • B7204S_v1_10028833
    • B7204S_v1_10032141
    • B7204S_v1_10032234
    • B7204S_v1_10034585
    • B7204S_v1_10037873
    • B7204S_v1_10038737
    • B7204S_v1_10039418
    • B7204S_v1_10037084
    • B7204S_v1_10043025
    • B7204S_v1_10045641
    • B7204S_v1_10048294
    • B7204S_v1_10050604
    • B7204S_v1_10050486
    • B7204S_v1_10052527
    • B7204S_v1_10055577
    • B7204S_v1_10056120
    • B7204S_v1_10056880
    • B7204S_v1_10055312
    • B7204S_v1_10057666
    • B7204S_v1_10062819
    • B7204S_v1_10056381
    • B7204S_v1_10063365
    • B7204S_v1_10065370
    • B7204S_v1_10066490
    • B7204S_v1_10064163
    • B7204S_v1_10067947
    • B7204S_v1_10070297
    • B7204S_v1_10072875
    • B7204S_v1_10076156
    • B7204S_v1_10079346
    • B7204S_v1_10075168
    • B7204S_v1_10080770
    • B7204S_v1_10078755
    • B7204S_v1_10082862
    • B7204S_v1_10083516
    • B7204S_v1_10084516
    • B7204S_v1_10082239
    • B7204S_v1_10085422
    • B7204S_v1_10090266
    • B7204S_v1_10090426
    • B7204G_v0_10091521
    • B7204S_v1_10090439
    • B7204G_v0_10090444
    • B7204S_v1_10092130
    • B7204S_v1_10092318
    • B7204S_v1_10092398
    • B7204S_v1_10091851
    • B7204G_v0_10093099
    • B7204S_v1_10093217
    • B7204S_v1_10095450
    • B7204S_v1_10097149
    • B7204S_v1_10098398
    • B7204S_v1_10098507
    • B7204S_v1_10098511
    • B7204S_v1_10118304
    • B7204S_v1_10121359

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • CutSmart® Buffer