Acyclonucleotide Set | NEB酶试剂 New England Biolabs

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Acyclonucleotide Set

Acyclonucleotide Set contains four separate tubes of acyNTPs (acyATP, acyCTP, acyGTP and acyTTP). 
Acyclonucleotides (acyNTPs) act as chain terminators and are thus useful in applications that normally employ dideoxynucleotides such as DNA sequencing (1,2) and SNP detection (3). AcyNTPs are especially useful in applications with archaeon DNA Polymerases, more specifically with Therminator™ DNA Polymerase. Therminator DNA Polymerase is an engineered enzyme with an increased capacity to incorporate analogs with altered sugars, such as ribonucleotides, dideoxynucleotides, 2´ deoxynucleotides and especially acyclo-base analogs (4,5). 

Acyclonucleotide Set | NEB酶试剂 New England Biolabs

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
N0460S 不适用 0.5 µmol ¥849.00 有 有 有 无 无
如何订购
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产品信息

When used with Therminator DNA Polymerase (NEB #M0261), the concentration of acyclo-base analog is roughly equivalent to dideoxynucleotide concentrations used with Thermo Sequenase™ (see figure).

Acyclonucleotide Set | NEB酶试剂 New England Biolabs
(50 ng/µl single-stranded M13mp18 DNA, 0.05 µM [32P]-S1224S primer, 1X ThermoPol Buffer, 50 µM dNTP, varying acyGTP and 0.05 units/µl Therminator) were mixed, incubated at 94°C for 5 minutes and then thermal cycled for 25 cycles at 94°C (30 seconds); 55°C (30 seconds); 72°C (30 seconds). Reaction products were separated on a denaturing polyacrylamide gel (QuickPoint, NOVEX, Inc.) and analyzed by autoradiography. Numbers above each lane give the molar ratio of acyGTP to dNTP in each lane (i.e., 50, 17, 5,6 and 1.9 µM acyGTP, respectively). Numbers to the side of the gel indicate the length of the primer extension product added to the primer.
产品类别:
Nucleotide Solutions Products

应用:
DNA Sequencing,
PCR,
PCR

  • 试剂盒组成

    试剂盒组成

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • N0460S     -20    
        AcycloATP N0461AVIAL -20 1 x 0.5 µmol 不适用
        AcycloCTP N0462AVIAL -20 1 x 0.5 µmol 不适用
        AcycloGTP N0463AVIAL -20 1 x 0.5 µmol 不适用
        AcycloTTP N0464AVIAL -20 1 x 0.5 µmol 不适用

  • 特性和用法

  • 相关产品

    相关产品

    • Therminator™ DNA Polymerase

  • 注意事项
    1. Thermo Sequenase™ is a trademark of GE Healthcare.
    2. Addition of 50 μl of distilled or deionized (Milli-Q™) water will result in a final concentration of 10 mM acyNTP. The hydrated acyNTP solution should subsequently be stored at -20°C.

  • 参考文献
    1. Sanger, F., Nicklen, S. and Coulson, A.R. (1977). Proc. Natl. Acad. Sci. USA. 74, 5463-5467.
    2. Trainor, G.L. (1996). U.S. Patent No. 5,558,991.
    3. Haff, L.A. and Simirnov, I.P. (1997). Genome Methods. 7, 378-388.
    4. Gardner, A.F. and Jack, W.E. (1999). Nucl. Acids Res.. 27, 2545-2553.
    5. Gardner, A.F. and Jack, W.E. (2002). Nucl. Acids Res.. 30(2), 605-613.

FAQs & 问题解决指南

  • FAQs
    1. Are substrates terminated with 3′ acyclonucleosides resistant to 3′-5′ exonuclease activity?
    2. Which DNA polymerases incorporate acyclonucleotides?
    3. Can acyclonucleotides be used in Sanger sequencing?
    4. Can acyclonucleotides be used for sequencing-by-synthesis?

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    Acyclonucleotide Set | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

  • 出版物
    • Gardner, A.F., Jackson, K.M., Boyle, M.M., Buss, J.A., Potapov, V., Gehring, A.M., Zatopek, K.M., Correa, I.R., Jr., Ong, J.L., Jack. W.E. (2019) Therminator DNA polymerase: Modified nucleotides and unnatural substrates Front Mol Biosci; 6, 28. PubMedID: 31069234​, DOI: 10.3389/fmolb.2019.00028

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • N0460S_v1

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • N0460S_v0_10013356
    • N0460S_v1_10013356
    • N0460S_v1_10022005
    • N0460S_v1_10048656
    • N0460S_v1_10092480
    • N0460S_v1_10123991
    • N0460S_v1_10151064
    • N0460S_v1_10154923
    • N0460S_v1_10157552
    • N0460S_v1_10163039

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • AcycloATP
    • AcycloCTP
    • AcycloGTP
    • AcycloTTP