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5-methyl-dCTP
Cytosine modification at carbon 5 (C5) represents an important epigenetic modification. It is also believed to be the starting substrate for the Ten-Eleven Translocation (TET) family of enzymes and their associated oxidation pathways.
- 5-methyl-dCTP (2′-deoxy-5-methylcytidine 5′-triphosphate) offers the ability to enzymatically make defined, fully methylated, cytosine-substituted DNA, which can be used for a variety of biochemical and cellular applications
- 5-methyl-dCTP is supplied as a 10 mM solution in Milli-Q™ water. Nucleotide concentration is determined by measurements of absorbance at 260 nm.
产品信息
- 产品类别:
- Nucleotide Solutions Products
- 应用:
- Histones & Nucleosome Assembly,
- DNA Sequencing,
- DNA Methylation Analysis,
- PCR,
- RT-PCR & cDNA Synthesis,
PCR
- 产品组分信息
产品组分信息
本产品提供以下试剂或组分:
NEB # 名称 组分货号 储存温度 数量 浓度 -
N0356S -20 5-methyl-dCTP N0356SVIAL -20 1 x 0.1 ml 10 mM
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- 特性和用法
提供
Milli-Q water as a triethylammonium salt
分子量
理论上的: 481.1 daltons
- 相关产品
相关产品
- Deoxynucleotide (dNTP) Solution Set
- 注意事项
- Supplied In:
Milli-Q™ water as an ammonium salt at (pH 7.0) - Formula:
C10H15N3O13P3 (free acid) - Diluent Compatibility:
Can be diluted using sterile distilled water, preferably Milli-Q™ water, or can be diluted using sterile TE (10 mM Tris-HCl, 1 mM EDTA (pH 7.5)).
- Supplied In:
FAQs & 问题解决指南
- FAQs
- Can 5-methyl-dCTP be used in in-vitro transcription reactions to make modified RNA?
引用 & 技术文献
- 引用文献
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质控、安全 & 法规
- 质控分析
每一新批次的 NEB 产品都要经过质控,以满足指定的质量标准,对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息,可从此处查阅。
- 产品说明与变更通知
产品说明与变更通知
产品说明表包含产品的储存温度、有效期和规格,这些文件的命名规则如下:[货号]_[规格]_[版本]
- N0356S_v1
- N0356S_v2
- CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控,这些文件的命名规则如下: [货号]_[规格]_[版本]_[Lot#]
- N0356S_v1_10031184
- N0356S_v1_10054779
- N0356S_v1_10077391
- N0356S_v1_10093418
- N0356S_v2_10109461
- SDS
以下 SDS 文件可以帮助您安全地使用该产品
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5-methyl-dCTP
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