上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
WarmStart® LAMP Kit (DNA & RNA)
Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets
Compatible with multiple detection methods
WarmStart® technology inhibits activity at room temperature
This reagent has been incorporated into Oxford Nanopore’s LamPORE technology, a combination of LAMP with nanopore sequencing, initially implemented to provide a rapid, low-cost and scalable assay for the detection of SARS-CoV-2.
Learn more about how RT-LAMP is being used for rapid COVID-19 screening
LAMP Fluorescent Dye (NEB #B1700) component available separately
精选视频
NEB® LAMP Primer Design Tool Tutorial
观看其他视频
产品信息
The WarmStart LAMP Kit (DNA & RNA) is designed to provide a simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provides rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. This kit is supplied with the WarmStart LAMP 2X Master Mix, which contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized LAMP buffer solution. Both Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase have been engineered for improved performance in LAMP and RT-LAMP reactions. A fluorescent dye is also supplied to enable real-time fluorescence measurement of LAMP. The WarmStart LAMP Kit is compatible with multiple detection methods, including turbidity detection, real-time fluorescence detection (when used with LAMP fluorescent dye) and end-point visualization.
The WarmStart LAMP Kit (DNA & RNA) is compatible with multiple detection methods
The WarmStart LAMP Kit (DNA & RNA) includes fluorescent dye for real-time fluorescence measurement. Alternately, detection can be accomplished by turbidity detection or a variety of endpoint visualization methods.
For carryover contamination prevention, a version containing dUTP and thermolabile UDG is available: WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG) (NEB #E1708).
A RNA target (HMBS2) was amplified from Jurkat total RNA using the WarmStart LAMP Kit and OptiGene Master Mix (ISO-001). Reactions were performed at 65°C for 74 minutes on a real-time thermocycler (Bio-Rad CFX96) in triplicate. Time to result is set as the time at which the fluorescence crossed a threshold of 10% of maximal fluorescence. NEB’s WarmStart LAMP Kit resulted in faster and more robust detection as compared to the OptiGene Master Mix.
Experiments were designed to amplify a DNA target (BRCAb) and a RNA target (HMBS2) by LAMP or RT-LAMP, respectively. A 25 μl reaction containing 10 ng HeLa DNA or 5 ng Jurkat total RNA, 1X Master Mix, 1X LAMP primers and fluorescent dye was incubated on a Bio-Rad CFX98 instrument at 65°C for 75 minutes. Lucigen® OmniAmp® RNA & DNA Kit and OptiGene Isothermal Master Mix (ISO-001 ) were run according to manufacturers’ recommendations.
NEB WarmStart LAMP Kit (DNA & RNA) enables specific amplification across multiple species/targets
PRIMER SET
DNA TEMPLATE
HeLa DNA
Lyme DNA
B. malayi DNA
E. coli DNA
C. elegans DNA
BRCAb (human)
+
CFTR (human)
+
LymO (Lyme)
+
BmHhal (B. malayi)
+
dnaE (E. coli)
+
Lec10b (C. elegans)
+
Lec6 (C. elegans)
+
In each individual reaction, multiple gDNA templates were added along with primers specific to a target in only one species. Reactions were incubated at 65°C for 74 minutes on a thermocycler (Bio-Rad CFX96) in triplicate. Positive results (+) were observed only in the expected species/target combinations, demonstrating high specificity of the WarmStart LAMP reactions.
LAMP Primers (we recommend using NEB LAMP Primer Design Tool)
Target Nucleic Acid Samples
Molecular Biology Grade H2O
Heat block, water bath, real-time turbidimeter or thermocycler (with real-time fluorescence measurement if desired) and instrument-appropriate reaction vessels
Powered by Bioz See more details on Bioz