WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) |NEB酶试剂 New England Biolabs

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WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA)

Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets

  • Fast, clear pink-to-yellow visible detection of amplification
  • WarmStart® feature inhibits activity at room temperature
  • Offered with dUTP and thermolabile UDG for carryover contamination prevention (NEB #M1804)
  • Read how NEB developed and implemented a SARS-CoV-2 RT-LAMP workflow for workplace surveillance: 1) NEB Expressions article 2) PLOS ONE publication
  • Learn about a colorimetric RT-LAMP assay used for the detection of Eastern equine encephalitis virus in mosquitoes: AMCA publication
  • A publication by members of the global LAMP (gLAMP) Consortium provides a comprehensive review of LAMP and its role in the COVID-19 pandemic

WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) |

Negative reactions are indicated in pink and positive reactions are indicated by a change to yellow.

精选视频

WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) |

NEB® LAMP Primer Design Tool Tutorial

观看其他视频

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M1800S 2 X 100 reactions (25 µl) ¥2,569.00 有 有 有 无 有
M1800L 2 X 500 reactions (25 µl) ¥10,269.00 无 无 无 无 无
如何订购
Please enter a quantity for at least one size

产品信息

WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) |

The WarmStart Colorimetric LAMP 2X Master Mix is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of Loop-Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions. This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow (an overview of LAMP and primer design can be found in our video library, https://www.neb.com/tools-and-resources/video-library). This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15–40 minutes. 

Simple Visualization of Amplification Success

WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) |

Negative reactions are indicated in pink and positive reactions are indicated by a change to yellow.
产品类别:
Isothermal Amplification & Strand Displacement Products

应用:
Loop-Mediated Isothermal Amplification,
Isothermal Amplification

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M1800S     -20    
        WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA) M1800SVIAL -20 1 x 1.25 ml 2 X
    • M1800L     -20    
        WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA) M1800SVIAL -20 5 x 1.25 ml 2 X

  • 特性和用法

  • 优势和特性

    应用特性

    • LAMP amplification of RNA and DNA targets
    • Simple visual indication of amplification for easy detection and analysis
    • One-step field and point-of-need LAMP reactions
    • Easy demonstration of LAMP assays with built-in detection

  • 相关产品

    相关产品

    • Bst 2.0 WarmStart DNA Polymerase
    • WarmStart® RTx Reverse Transcriptase
    • Isothermal Amplification Buffer Pack
    • Deoxynucleotide (dNTP) Solution Mix
    • WarmStart® LAMP Kit (DNA & RNA)
    • Bst 3.0 DNA Polymerase
    • M1804 WarmStart Colorimetric LAMP 2X Master Mix with UDG

操作说明、说明书 & 用法

  • 操作说明
    1. WarmStart Colorimetric LAMP 2X Master Mix Typical LAMP Protocol (M1800)

  • 应用实例
    • Optimized Integration of New England Biolabs® Loop-mediated Isothermal Amplification (LAMP) Reagents with Axxin ISO Instruments
    • Facilitating Detection of SARS-CoV-2 Directly from Patient Samples: Precursor Studies with RT-qPCR and Colorimetric RT-LAMP Reagents

工具 & 资源

  • Web 工具
    • NEB LAMP Primer Design Tool

FAQs & 问题解决指南

  • FAQs
    1. What is the difference between using Colorimetric LAMP and regular LAMP reactions?
    2. How long should I incubate the Colorimetric LAMP reaction before checking the results?
    3. What components are contained in the WarmStart® Colorimetric LAMP 2X Master Mix and what else do I need to perform a LAMP reaction?
    4. What is the Mg++ concentration in the Colorimetric LAMP 2X Master Mix? 
    5. Can the Colorimetric LAMP 2X Master Mix be used in instruments that utilize fluorescent detection of DNA amplification or real time turbidity measurement?
    6. How do I design LAMP Primers?
    7. The WarmStart LAMP Master Mix has some precipitation in the tube after thawing, is this normal?
    8. How stable are the WarmStart Colorimetric LAMP 2X Master Mixes?
    9. What buffer should I use for my target samples and primers going into the colorimetric LAMP reaction?
    10. What components are contained in the WarmStart Colorimetric LAMP Master Mix and what else I need to perform a LAMP reaction?
    11. What is the difference between the WarmStart® Colorimetric LAMP 2X Master Mix with UDG (NEB #M1804) and the WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) (NEB #M1800)?
    12. What if my colorimetric LAMP mix is orange instead of pink prior to amplification?
    13. Should I use Guanidine HCl (B2619A) in colorimetric LAMP reactions?
    14. How much sample can be added to the colorimetric LAMP reaction as input?
    15. Can I monitor the color change of the colorimetric LAMP reactions using a spectrophotometer?
    16. Can the WarmStart Colorimetric LAMP 2X Master Mixes be used in instruments that utilize fluorescence detection?
    17. What is the optimal LAMP/RT-LAMP amplification temperature?
    18. How fast should I expect a result?
    19. Can I set up my LAMP/RT-LAMP reactions at room temperature?
    20. Amplification occurred in my NTC sample(s) following isothermal incubation. What happened?
    21. Is a separate incubation step required for RT-LAMP?
    22. Does NEB have a master mix for LAMP or RT-LAMP reactions?
    23. What is LAMP and RT-LAMP?

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) | Powered by Bioz See more details on Bioz

  • 专题文章
    • Using aptamers to control enzyme activities: Hot Start Taq and beyond
    • Many Initiatives Turning to RT-LAMP as Alternative to PCR for Rapid COVID-19 Screening Assays

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    Change Notifications

    Product is now supplied in Sarstedt vials (February 28, 2022).

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • M1800S_L_v3

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • M1800S_L_v1_0071706
    • M1800S_L_v1_0091708
    • M1800S_L_v1_0111712
    • M1800S_L_v1_0121803
    • M1800S_v1_10011926
    • M1800S_v1_10012571
    • M1800S_v1_10017719
    • M1800L_v1_10012573
    • M1800S_v1_10028159
    • M1800S_v1_10035205
    • M1800L_v1_10037163
    • M1800S_v1_10037165
    • M1800S_v1_10039386
    • M1800S_v1_10039425
    • M1800L_v1_10039920
    • M1800S_v2_10042047
    • M1800L_v2_10044056
    • M1800L_v2_10047874
    • M1800L_v2_10048419
    • M1800S_v2_10048414
    • M1800L_v2_10049943
    • M1800S_v2_10053932
    • M1800L_v2_10055829
    • M1800L_v2_10060133
    • M1800L_v2_10062110
    • M1800S_v2_10055830
    • M1800S_v2_10064419
    • M1800S_v2_10065108
    • M1800L_v2_10064065
    • M1800L_v2_10067885
    • M1800L_v3_10068156
    • M1800L_v3_10068562
    • M1800L_v3_10069263
    • M1800L_v3_10070831
    • M1800L_v3_10071284
    • M1800S_v3_10071282
    • M1800L_v3_10072328
    • M1800S_v3_10072338
    • M1800L_v3_10072980
    • M1800L_v3_10074036
    • M1800S_v3_10075064
    • M1800L_v3_10075065
    • M1800L_v3_10076522
    • M1800S_v3_10077350
    • M1800L_v3_10085101
    • M1800S_v3_10082799
    • M1800S_v3_10088115
    • M1800L_v3_10088114
    • M1800L_v3_10069646
    • M1800S_v3_10089258
    • M1800L_v3_10090997
    • M1800S_v3_10093929
    • M1800L_v3_10096861
    • M1800L_v3_10108532
    • M1800L_v3_10109573
    • M1800S_v3_10100852
    • M1800S_v3_10109764
    • M1800L_v3_10115578
    • M1800L_v3_10117502
    • M1800L_v3_10120599
    • M1800S_v3_10117826
    • M1800L_v3_10127400
    • M1800S_v3_10140977
    • M1800L_v3_10141526
    • M1800S_v3_10148694
    • M1800S_v3_10156440
    • M1800L_v3_10155445
    • M1800S_v3_10161057

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA)