Deep Vent® DNA Polymerase | NEB酶试剂 New England Biolabs

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Deep Vent® DNA Polymerase

  • Deep Vent® offers moderate fidelity and robust performance
  • Extremely high thermostability: half-life of 23 hours at 95°C
  • 5X higher fidelity than Taq
  • Difficult templates: ideal for GC-rich or looped sequence
  • Extremely High Thermostability: half-life of 23 hours at 95°C

Deep Vent® DNA Polymerase | NEB酶试剂 New England Biolabs

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0258S 2,000 units/ml 200 units ¥1,129.00 无 无 有 无 无
M0258L 2,000 units/ml 1,000 units ¥4,509.00 无 无 无 无 无
如何订购
Please enter a quantity for at least one size

Deep Vent® DNA Polymerase | NEB酶试剂 New England Biolabs Need a custom/large volume order? Contact Us

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产品信息

Deep Vent DNA Polymerase was the second high-fidelity thermophilic DNA polymerase available from New England Biolabs. The fidelity of Deep Vent DNA Polymerase is derived in part from an integral 3´→ 5´ proofreading exonuclease activity. Deep Vent is even more stable than Vent at temperatures of 95 to 100°C.

Deep Vent® DNA Polymerase | NEB酶试剂 New England Biolabs
Deep Vent DNA Polymerase crystals (Ira Schildkraut and Rebecca Kucera, New England Biolabs, Inc.)
Deep Vent® DNA Polymerase | NEB酶试剂 New England Biolabs Amplification of Jurkat genomic DNA with Deep Vent DNA Polymerase. Amplicon sizes are indicated below gel. Marker M is the 1 kb DNA Ladder (NEB #N3232 ).

产品来源

An E. coli strain that carries the Deep Vent® DNA Polymerase gene from Pyrococcus species GB-D. The native organism was isolated from a submarine thermal vent at 2,010 meters (1) and is able to grow at temperatures as high as 104°C.

产品类别:
Other PCR Polymerases

应用:
Routine PCR,
PCR

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0258S     -20    
        Deep Vent® DNA Polymerase M0258SVIAL -20 1 x 0.1 ml 2,000 units/ml
        ThermoPol® Reaction Buffer Pack B9004SVIAL -20 1 x 1.5 ml 10 X
        Magnesium Sulfate (MgSO4) Solution B1003SVIAL -20 1 x 1.5 ml 100 mM
    • M0258L     -20    
        Deep Vent® DNA Polymerase M0258LVIAL -20 1 x 0.5 ml 2,000 units/ml
        ThermoPol® Reaction Buffer Pack B9004SVIAL -20 3 x 1.5 ml 10 X
        Magnesium Sulfate (MgSO4) Solution B1003SVIAL -20 1 x 1.5 ml 100 mM

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.

    反应条件

    1X ThermoPol® Reaction Buffer Pack

    1X ThermoPol® Reaction Buffer Pack
    20 mM Tris-HCl
    10 mM (NH4)2SO4
    10 mM KCl
    2 mM MgSO4
    0.1% Triton® X-100
    (pH 8.8 @ 25°C)

    贮存溶液

    10 mM Tris-HCl
    100 mM KCl
    1 mM DTT
    0.1 mM EDTA
    50% Glycerol
    0.1% Triton® X-100
    pH 7.4 @ 25°C

    热失活

    否

    分子量

    理论上的: 89000 daltons

    5′ – 3′ 核酸外切酶

    No

    3′ – 5′ 核酸外切酶

    Yes

    链置换

    ++

    产物末端

    • Blunt Ends

    单位活性检测条件

    1X ThermoPol Reaction Buffer, 200 µM each dNTP including [3H]-dTTP and 15 nM primed M13 DNA. 

  • 优势和特性

    Features

    • Ideal for GC-rich or looped sequences

    应用特性

    • Primer extension
    • PCR

  • 相关产品

    相关产品

    • Deoxynucleotide (dNTP) Solution Mix
    • Deoxynucleotide (dNTP) Solution Set

    单独销售的组分

    • ThermoPol® Reaction Buffer Pack
    • Magnesium Sulfate (MgSO4) Solution

  • 注意事项
    1. BSA is not provided with this enzyme since its presence is not necessary for most primer extension reactions. However, it is available as NEB #B9001. Acetylated BSA should not be used for primer extension reactions.
    2. The calculated half-life of Deep Vent DNA Polymerase at 95°C is 23 hours.
    3. Diluent D is also available (NEB# B8004). This diluent is recommended for making dilutions of Deep Vent Polymerase.
    4. Additional ThermoPol Reaction Buffer packs for this product are also available (NEB# B9004). Each buffer pack contains 4 vials of 10X buffer (1.5 mls each) and 1 vial of 100 mM MgSO4.

  • 参考文献
    1. Jannasch, H.W. et al. (1992). Appl. Environ. Microbiol.. 58, 3472-3481.

操作说明、说明书 & 用法

  • 操作说明
    1. Guidelines for PCR Optimization for Deep Vent® DNA Polymerase
    2. Protocol for a Routine Deep Vent® PCR

  • 使用指南
    • Guidelines for PCR Optimization with Thermophilic DNA Polymerases

工具 & 资源

  • 选择指南
    • DNA Polymerase Selection Chart
    • Thermophilic DNA Polymerases

FAQs & 问题解决指南

  • FAQs
    1. Can Deep Vent DNA Polymerase be used in other buffers?
    2. I can’t get Deep Vent DNA Polymerase to work yet Taq DNA Polymerase works fine.
    3. Are the DNA fragments produced by Deep Vent DNA Polymerase blunt-ended or do they have the single-base 3′ overhang that Taq DNA Polymerase yields?
    4. Can I use Deep Vent DNA Polymerase to polish the ends of DNA fragments?
    5. I want to clone primer extension products made with Vent DNA Polymerase or Deep Vent DNA Polymerase. Are some protocols better than others?
    6. Can Vent® DNA Polymerase (NEB# M0254) or Deep Vent® DNA Polymerase (NEB# M0258) be used to create blunt ends on primer extension products made with Taq DNA Polymerase (NEB# M0267)?

  • 问题解决指南
    • PCR Troubleshooting Guide

  • 实验技巧
    The amount of enzyme in the reaction is critical. Try a final concentration of 0.01 U/µl Deep Vent DNA Polymerase in the reaction.

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    Deep Vent® DNA Polymerase | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • M0258S_L_v2

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • M0258S_L_v1_0181512
    • M0258S_L_v1_0181606
    • M0258S_L_v1_0181612
    • M0258S_L_v1_0181702
    • M0258S_L_v1_0181706
    • M0258S_L_v1_0181712
    • M0258L_v2_10015886
    • M0258S_v2_10017725
    • M0258L_v2_10028994
    • M0258L_v2_10034648
    • M0258S_v2_10034093
    • M0258L_v2_10054254
    • M0258L_v2_10057547
    • M0258S_v2_10055405
    • M0258S_v2_10062085
    • M0258L_v2_10069472
    • M0258S_v2_10073242
    • M0258L_v2_10107186
    • M0258S_v2_10105665
    • M0258S_v2_10122364
    • M0258L_v2_10126419
    • M0258S_v2_10138863
    • M0258S_v2_10151856
    • M0258L_v2_10156575

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • Deep Vent® DNA Polymerase
    • ThermoPol® Reaction Buffer Pack
    • Magnesium Sulfate (MgSO4) Solution