LongAmp® Taq PCR Kit |NEB酶试剂 New England Biolabs

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LongAmp® Taq PCR Kit

Exceptional performance with long amplicons

  • Optimized enzyme blend for amplification up to 30 kb from low complexity templates and difficult templates, such has human genomic DNA
  • 2X higher fidelity than Taq
  • Kit contains 5X Reaction Buffer for optimal performance

精选视频

LongAmp® Taq PCR Kit |

Tips for Amplifying Large Amplicons

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库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
E5200S 不适用 100 reactions (50 µl) ¥1,349.00 有 有 无 无 无
如何订购
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产品信息

The LongAmp Taq PCR Kit features LongAmp Taq DNA Polymerase, which is a unique blend of Taq and Deep Vent® DNA Polymerases. The 3´→5´ exonuclease activity of Deep Vent® Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). LongAmp Taq DNA Polymerase offers two fold higher fidelity than Taq Polymerase alone. The LongAmp Taq PCR Kit is supplied with LongAmp Taq Reaction Buffer, which is optimized to give highest PCR yields up to 30 kb. The kit can generate a wide range of PCR products, up to 30 kb from plasmid or from human genomic DNA.

LongAmp® Taq PCR Kit |
Amplification of specific sequences from human genomic DNA using LongAmp Taq DNA Polymerase. Amplicon sizes are indicated below the gel. Marker M is NEB 1 kb DNA Ladder (NEB #N3232).
产品类别:
LongAmp® DNA Polymerases Products

应用:
Long Range PCR,
Specialty PCR,
Routine PCR,

PCR

  • 试剂盒组成

    Kit 组分

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • E5200S     -20    
        LongAmp® Taq DNA Polymerase M0323SVIAL -20 1 x 0.2 ml 2,500 units/ml
        Deoxynucleotide (dNTP) Solution Mix N0447SVIAL -20 1 x 0.2 ml 10 mM each dNTP
        LongAmp® Taq Reaction Buffer Pack B0323SVIAL -20 1 x 1.5 ml 5 X
        Magnesium Sulfate (MgSO4) Solution B1003SVIAL -20 1 x 1.5 ml 100 mM
        Nuclease-free Water B1502AVIAL -20 2 x 1.5 ml 不适用

  • 特性和用法

  • 优势和特性

    应用特性

    • Long Range PCR
    • Colony PCR

  • 相关产品

    相关产品

    • Deoxynucleotide (dNTP) Solution Set
    • LongAmp® Taq DNA Polymerase
    • Magnesium Sulfate (MgSO4) Solution
    • LongAmp® Hot Start Taq 2X Master Mix
    • LongAmp® Hot Start Taq DNA Polymerase

  • 参考文献
    1. Barnes, W.M. (1994). Proc. Natl. Acad. Sci. 91, 2216-2220.
    2. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
    3. Powell, L.M. et al. (1987). Cell. 50, 831-840.
    4. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
    5. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res. 18, 7465.

操作说明、说明书 & 用法

  • 操作说明
    1. Protocol for LongAmp™ Taq PCR Kit

  • 使用指南
    • Activity of Restriction Enzymes in PCR Buffers
    • Guidelines for PCR Optimization with Taq DNA Polymerase
    • Guidelines for PCR Optimization with Thermophilic DNA Polymerases

工具 & 资源

  • 选择指南
    • DNA Polymerase Selection Chart
    • Thermophilic DNA Polymerases

  • Web 工具
    • Tm Calculator

FAQs & 问题解决指南

  • FAQs
    1. What is the recommended enzyme amount when using LongAmp™ Taq DNA Polymerase?
    2. Can the extension step be carried out at 72°C when using LongAmp™ Taq DNA Polymerase?
    3. What is the extension rate when using LongAmp™ Taq DNA Polymerase?
    4. Why is the product a smear when visualized on an agarose gel?
    5. Can the LongAmp™ kit be used to amplify GC-rich amplicons?
    6. How is OneTaq DNA Polymerase different from LongAmp™ Taq DNA Polymerase?
    7. What type of DNA ends result from a primer extension reaction or a PCR using LongAmp™ Taq DNA Polymerase?
    8. What is the fidelity of the LongAmp™ Taq DNA Polymerase compared to Taq DNA Polymerase?
    9. Can NEBuilder® HiFi DNA Assembly master mix remove both 3′ and 5′ end mismatches?

  • 问题解决指南
    • PCR Troubleshooting Guide
    • Taq PCR Kit Troubleshooting Guide

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    LongAmp® Taq PCR Kit | Powered by Bioz See more details on Bioz

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • E5200S_v1

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • E5200S_v1_0411712
    • E5200S_v1_10013129
    • E5200S_v1_10015908
    • E5200S_v1_10024730
    • E5200S_v1_10029415
    • E5200S_v1_10034811
    • E5200S_v1_10043958
    • E5200S_v1_10052221
    • E5200S_v1_10061500
    • E5200S_v1_10067259
    • E5200S_v1_10069146
    • E5200S_v1_10073017
    • E5200S_v1_10080294
    • E5200S_v1_10088161
    • E5200S_v1_10092965
    • E5200S_v1_10100863
    • E5200S_v1_10108304
    • E5200S_v1_10112948
    • E5200S_v1_10119049
    • E5200S_v1_10135893
    • E5200S_v1_10147001
    • E5200S_v1_10159710

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • LongAmp® Taq DNA Polymerase
    • Deoxynucleotide (dNTP) Solution Mix
    • Magnesium Sulfate (MgSO4) Solution
    • Nuclease-free Water