LongAmp® Taq 2X Master Mix | NEB酶试剂 New England Biolabs

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LongAmp® Taq 2X Master Mix

Exceptional performance with long amplicons

  • Optimized enzyme blend for amplification up to 30 kb
  • 2X higher fidelity than Taq
  • Master mix is a 2X concentrated solution containing everything needed for robust amplification of complex or simple templates

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LongAmp® Taq 2X Master Mix | NEB酶试剂 New England Biolabs

Tips for Amplifying Large Amplicons

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库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0287S 2 X 100 reactions (50 µl) ¥1,549.00 有 有 有 有 无
M0287L 2 X 500 reactions (50 µl) ¥6,179.00 有 有 无 无 无
如何订购
Please enter a quantity for at least one size

产品信息

The LongAmp® Taq 2X Master Mix combines high quality NEB recombinant Taq DNA Polymerase with a trace amount of Deep Vent® DNA Polymerase. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). This ready-to-use mix offers two fold higher fidelity than Taq DNA Polymerase alone. A wide range of PCR products can be generated, up to 30 kb from lambda or human genomic DNA.

LongAmp® Taq 2X Master Mix | NEB酶试剂 New England Biolabs
Amplification of specific sequences from human genomic DNA using LongAmp Taq DNA Polymerase. Amplicon sizes are indicated below the gel. Marker M is the NEB 1 kb DNA Ladder (NEB #N3232 ).

产品来源

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent® DNA Polymerase gene from Pyrococcus species GB-D.

产品类别:
LongAmp® DNA Polymerases Products,
Master Mixes Products

应用:
Long Range PCR,
Specialty PCR,
Routine PCR,

PCR

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0287S     -20    
        LongAmp® Taq 2X Master Mix M0287SVIAL -20 2 x 1.25 ml 2 X
    • M0287L     -20    
        LongAmp® Taq 2X Master Mix M0287SVIAL -20 10 x 1.25 ml 2 X

  • 特性和用法

    1X 预混液组分

    60 mM Tris-SO4
    20 mM (NH4)2SO4
    2 mM MgSO4
    0.3 mM dNTPs
    3% Glycerol
    0.06% IGEPAL® CA-630
    0.05% Tween® 20
    125 units/ml LongAmp Taq DNA Polymerase
    pH 9.1 @ 25°C

    热失活

    否

    5′ – 3′ 核酸外切酶

    Yes

    3′ – 5′ 核酸外切酶

    Yes

    链置换

    +

    单位活性检测条件

    1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.

    Reaction Conditions: 
    1X LongAmp Taq Master Mix, DNA template and primers in a total reaction volume of 25–50 µl.

    Unit Definition:
    One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

  • 优势和特性

    应用特性

    • Long Range PCR
    • High Throughout PCR
    • Colony PCR

  • 相关产品

    相关产品

    • Deoxynucleotide (dNTP) Solution Mix
    • Deoxynucleotide (dNTP) Solution Set
    • LongAmp® Taq DNA Polymerase
    • LongAmp® Taq PCR Kit
    • Magnesium Sulfate (MgSO4) Solution
    • LongAmp® Hot Start Taq 2X Master Mix
    • LongAmp® Hot Start Taq DNA Polymerase

  • 注意事项
    1. LongAmp Taq 2X Master Mix is stable for fifteen freeze-thaw cycles when stored at -20°C.
    2. LongAmp Taq 2X Master Mix is also stable for three months at 4°C, so for frequent use, an aliquot may be kept at 4°C.

  • 参考文献
    1. Barnes, W. M. (1994). Proc. Natl. Acad. Sci. USA. 91, 2216-2220.
    2. Saiki R. K. et al. (1985). Science. 230, 1350-1354.
    3. Powell, L. M. et al. (1987). Cell. 50, 831-840.
    4. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
    5. Sarkar, G., Kapelner, S. and Sommer, S. S. (1990). Nucleic Acids Res. 18, 7465.

操作说明、说明书 & 用法

  • 操作说明
    1. Protocol for LongAmp™ Taq 2X Master Mix

  • 使用指南
    • Activity of Restriction Enzymes in PCR Buffers
    • Guidelines for PCR Optimization with Taq DNA Polymerase
    • Guidelines for PCR Optimization with Thermophilic DNA Polymerases

工具 & 资源

  • 选择指南
    • DNA Polymerase Selection Chart
    • Thermophilic DNA Polymerases

  • Web 工具
    • Tm Calculator

FAQs & 问题解决指南

  • FAQs
    1. What is the fidelity of the LongAmp™ Taq DNA Polymerase compared to Taq DNA Polymerase?
    2. What type of DNA ends result from a primer extension reaction or a PCR using LongAmp™ Taq DNA Polymerase?
    3. What is the extension rate when using LongAmp™ Taq DNA Polymerase?
    4. Can the extension step be carried out at 72°C when using LongAmp™ Taq DNA Polymerase?
    5. Can LongAmp™ Taq DNA Polymerase be used to amplify GC-rich amplicons?
    6. Is Monarch-purified gDNA suitable for long-range PCR?

  • 问题解决指南
    • PCR Troubleshooting Guide
    • Taq PCR Kit Troubleshooting Guide

  • 实验技巧
    Using an extension temperature of 65°C is recommended in LongAmp Taq reactions.

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    LongAmp® Taq 2X Master Mix | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • M0287S_L_v1
    • M0287S_L_v2

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • M0287S_L_v1_0271603
    • M0287S_L_v1_0291608
    • M0287S_L_v1_0301702
    • M0287S_L_v1_0321708
    • M0287S_L_v1_0331802
    • M0287L_v1_10015290
    • M0287S_v1_10015662
    • M0287L_v1_10018988
    • M0287L_v1_10019217
    • M0287S_v1_10024153
    • M0287L_v1_10025856
    • M0287S_v1_10031777
    • M0287L_v1_10034132
    • M0287S_v1_10034133
    • M0287L_v1_10036832
    • M0287S_v1_10038159
    • M0287L_v1_10041512
    • M0287S_v1_10041789
    • M0287S_v1_10046049
    • M0287L_v1_10048420
    • M0287L_v1_10049788
    • M0287S_v1_10049786
    • M0287L_v1_10051883
    • M0287S_v1_10052112
    • M0287L_v1_10055343
    • M0287S_v1_10056296
    • M0287L_v1_10057675
    • M0287L_v1_10062064
    • M0287S_v1_10062065
    • M0287L_v2_10065904
    • M0287S_v2_10065909
    • M0287L_v2_10070252
    • M0287L_v2_10071793
    • M0287S_v2_10071794
    • M0287L_v2_10077337
    • M0287S_v2_10080299
    • M0287L_v2_10080306
    • M0287L_v2_10084988
    • M0287L_v2_10087405
    • M0287S_v2_10084989
    • M0287S_v2_10088963
    • M0287L_v2_10093300
    • M0287S_v2_10093299
    • M0287L_v2_10097874
    • M0287S_v2_10097875
    • M0287L_v2_10101074
    • M0287S_v2_10101209
    • M0287L_v2_10106249
    • M0287S_v2_10104858
    • M0287S_v2_10106912
    • M0287L_v2_10111724
    • M0287S_v2_10111723
    • M0287S_v2_10116770
    • M0287L_v2_10115880
    • M0287S_v2_10119090
    • M0287L_v2_10121538
    • M0287S_v2_10122307
    • M0287S_v2_10129528
    • M0287L_v2_10124118
    • M0287S_v2_10132277
    • M0287L_v2_10123494
    • M0287L_v2_10135769
    • M0287S_v2_10137220
    • M0287L_v2_10143139
    • M0287S_v2_10143233
    • M0287L_v2_10146122
    • M0287S_v2_10149749
    • M0287L_v2_10152161
    • M0287S_v2_10157008
    • M0287L_v2_10157164
    • M0287L_v2_10159732
    • M0287S_v2_10159704

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • LongAmp® Taq 2X Master Mix