LongAmp® Hot Start Taq DNA Polymerase | NEB酶试剂 New England Biolabs

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LongAmp® Hot Start Taq DNA Polymerase

LongAmp® Hot Start Taq DNA Polymerase offers exceptional performance with long amplicons.

  • Aptamer-based inhibitor enables room temperature reaction setup
  • Optimized enzyme blend for amplification up to 30 kb
  • 2X higher fidelity than Taq
  • Master mix version available

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LongAmp® Hot Start Taq DNA Polymerase | NEB酶试剂 New England Biolabs

Tips for Amplifying Large Amplicons

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库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0534S 2,500 units/ml 500 units ¥1,329.00 有 有 无 无 有
M0534L 2,500 units/ml 2,500 units ¥5,299.00 无 无 无 无 无
如何订购
Please enter a quantity for at least one size

产品信息

LongAmp Hot Start Taq DNA Polymerase is a unique blend of aptamer-based Hot Start Taq and Deep Vent® DNA Polymerases. The aptamer-based inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of PCR reactions at room temperature. An advantage of the aptamer-based hot start mechanism is that it does not require a separate high temperature incubation step to activate the enzyme. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Hot Start Taq DNA Polymerase (1). LongAmp Hot Start Taq DNA Polymerase offers two-fold higher fidelity than Hot Start Taq DNA Polymerase alone. A wide range of PCR products can be generated; up to 30 kb from lambda or human genomic DNA.

LongAmp® Hot Start Taq DNA Polymerase | NEB酶试剂 New England Biolabs
Amplification of a 6 kb amplicon from varying amounts of human genomic DNA using LongAmp Hot Start Taq DNA Polymerase. Starting template amounts are indicated below the gel. Marker M is the NEB 2-Log DNA Ladder (NEB #N3200)

产品来源

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent® DNA Polymerase gene from Pyrococcus species GB-D.

产品类别:
LongAmp® DNA Polymerases Products

应用:
Hot Start PCR,
Long Range PCR,
Specialty PCR,

Routine PCR,

PCR

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0534S     -20    
        LongAmp® Hot Start Taq DNA Polymerase M0534SVIAL -20 1 x 0.2 ml 2,500 units/ml
        LongAmp® Taq Reaction Buffer Pack B0323SVIAL -20 1 x 1.5 ml 5 X
    • M0534L     -20    
        LongAmp® Hot Start Taq DNA Polymerase M0534LVIAL -20 1 x 1 ml 2,500 units/ml
        LongAmp® Taq Reaction Buffer Pack B0323SVIAL -20 4 x 1.5 ml 5 X

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.

    反应条件

    1X LongAmp® Taq Reaction Buffer Pack

    1X LongAmp® Taq Reaction Buffer Pack
    60 mM Tris-SO4
    20 mM (NH4)2SO4
    2 mM MgSO4
    3% Glycerol
    0.06% IGEPAL® CA-630
    0.05% Tween® 20
    (pH 9.1 @ 25°C)

    贮存溶液

    10 mM Tris-HCl
    100 mM KCl
    1 mM DTT
    0.1 mM EDTA
    0.5% Tween® 20
    0.5% IGEPAL® CA-630
    50% Glycerol
    pH 7.4 @ 25°C

    热失活

    否

    5′ – 3′ 核酸外切酶

    Yes

    3′ – 5′ 核酸外切酶

    Yes

    链置换

    +

    单位活性检测条件

    1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.

  • 优势和特性

    Features

     

    应用特性

     

  • 相关产品

    相关产品

    • Deoxynucleotide (dNTP) Solution Set
    • Deoxynucleotide (dNTP) Solution Mix
    • LongAmp® Taq PCR Kit
    • Magnesium Sulfate (MgSO4) Solution
    • LongAmp® Taq DNA Polymerase
    • LongAmp® Hot Start Taq 2X Master Mix
    • LongAmp® Taq 2X Master Mix

    单独销售的组分

    • LongAmp® Taq Reaction Buffer

  • 注意事项
    1. 5’→3′ flap endonuclease destroys displaced strand.

  • 参考文献
    1. Barnes, W.M. (1994). Proc.Natl. Acad. Sci. USA. 91, 2216-2220.
    2. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
    3. Powell, L.M. et al. (1987). Cell. 50, 831-840.
    4. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
    5. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res.. 18, 7465.

操作说明、说明书 & 用法

  • 操作说明
    1. PCR Protocol for LongAmp® Hot Start Taq DNA Polymerase (M0534)

  • 使用指南
    • Activity of Restriction Enzymes in PCR Buffers
    • Guidelines for PCR Optimization with Taq DNA Polymerase
    • Guidelines for PCR Optimization with Thermophilic DNA Polymerases

工具 & 资源

  • 选择指南
    • DNA Polymerase Selection Chart
    • Thermophilic DNA Polymerases

  • Web 工具
    • Tm Calculator

FAQs & 问题解决指南

  • FAQs
    1. What is the recommended enzyme amount when using LongAmp™ Hot Start Taq DNA Polymerase?
    2. What is the fidelity of the LongAmp™ Hot Start Taq DNA Polymerase compared to Taq DNA Polymerase?
    3. Can the extension step be carried out at 72°C when using LongAmp™ Hot Start Taq DNA Polymerase?
    4. What type of DNA ends result from a primer extension reaction or a PCR using LongAmp™ Hot Start Taq DNA Polymerase?
    5. What is the extension rate when using LongAmp™ Hot Start Taq DNA Polymerase?
    6. Why is the product a smear when visualized on an agarose gel?
    7. Can LongAmp™ Hot Start Taq DNA Polymerase be used to amplify GC-rich amplicons?
    8. Is Monarch-purified gDNA suitable for long-range PCR?

  • 问题解决指南
    • PCR Troubleshooting Guide
    • Taq PCR Kit Troubleshooting Guide

  • 实验技巧
    Using an extension temperature of 65°C is recommended in LongAmp Taq reactions

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    LongAmp® Hot Start Taq DNA Polymerase | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

  • 专题文章
    • Using aptamers to control enzyme activities: Hot Start Taq and beyond

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • M0534S_L_v1
    • M0534S_L_v2

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • M0534S_L_v1_0061512
    • M0534S_L_v1_0071606
    • M0534S_L_v1_0071612
    • M0534S_L_v1_0071712
    • M0534S_v1_10015647
    • M0534L_v1_10016146
    • M0534S_v1_10024742
    • M0534L_v1_10029420
    • M0534L_v1_10037245
    • M0534S_v1_10048518
    • M0534L_v1_10048517
    • M0534S_v1_10060285
    • M0534L_v2_10069476
    • M0534S_v2_10074203
    • M0534L_v2_10075651
    • M0534S_v2_10099468
    • M0534L_v2_10108227
    • M0534S_v2_10109943
    • M0534L_v2_10117357
    • M0534L_v2_10122834
    • M0534S_v2_10125365
    • M0534L_v2_10140976
    • M0534S_v2_10138865

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • LongAmp® Hot Start Taq DNA Polymerase