OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer | NEB酶试剂 New England Biolabs

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OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases for use with routine and difficult PCR experiments.

• Unique hot start formulation eliminates nonspecific amplification and does not require a separate activation step

• Obtain high yields with GC rich templates (>65%)

• Offered with inert tracking dyes for easy and direct loading of PCR products onto gels

OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer | NEB酶试剂 New England Biolabs

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0489S 2 X 100 reactions (50 µl) ¥719.00 有 无 无 无 无
M0489L 2 X 500 reactions (50 µl) ¥3,249.00 无 无 无 无 无
如何订购
Please enter a quantity for at least one size

产品信息

OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer is an optimized, ready-to-use blend of Taq and Deep Vent® DNA Polymerases combined with an aptamer-based inhibitor. This enzyme blend is ideally suited to PCR applications from GC-rich templates, including pure DNA solutions, bacterial colonies, and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient master mix formulation contains dNTPs, MgSO4, buffer components and stabilizers as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask co-migrating DNA bands.

Comparison of OneTaq Products to Other Commercially Available Polymerases

OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer | NEB酶试剂 New England Biolabs 

Amplification of a selection of high GC human genomic DNA targets demonstrates OneTaq performance. All polymerases were cycled according to manufacturer’s recommendations, including the use of additives to enhance the amplification of targets with high GC content. Yield (dot size) and purity (color) of reaction product were quantified from triplicate reactions on a Perkin Elmer LabChip®. A large, dark green dot represents the highest yield and purity.

OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer | NEB酶试剂 New England Biolabs

重点

产品来源

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent® DNA Polymerase gene from Pyrococcus species GB-D.

产品类别:
OneTaq® DNA Polymerases Products,
Taq DNA Polymerase Products,
Master Mixes Products

应用:
Hot Start PCR,
Multiplex PCR,
Specialty PCR,

Routine PCR,

PCR

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0489S     -20    
        OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer M0489SVIAL -20 2 x 1.25 ml 2 X
        OneTaq® High GC Enhancer B9026AVIAL -20 1 x 2 ml 不适用
    • M0489L     -20    
        OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer M0489SVIAL -20 10 x 1.25 ml 2 X
        OneTaq® High GC Enhancer B9026AVIAL -20 3 x 2 ml 不适用

  • 特性和用法

    1X 预混液组分

    80 mM Tris-SO4
    1X Tartrazine
    25 units/ml OneTaq Hot Start DNA Polymerase
    20 mM (NH4)2SO4
    0.2 mM dNTPs
    2 mM MgSO4
    5% Glycerol
    5% DMSO
    0.06% IGEPAL® CA-630
    0.05% Tween® 20
    1X Xylene Cyanol
    pH 9.2 @ 25°C

    热失活

    否

    单位活性检测条件

    1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.

    Unit Definition:
    One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.

    Reaction Conditions:
    1X OneTaq Hot Start Quick-Load Master Mix with GC Buffer, DNA template and primers in a total reaction volume of 50 µl.

  • 优势和特性

    应用特性

    • GC-rich PCR
    • High Sensitivity PCR
    • High Throughput PCR
    • Colony PCR
    • Long PCR (up to ~6 kb genomic)

  • 相关产品

    相关产品

    • Magnesium Sulfate (MgSO4) Solution
    • OneTaq® DNA Polymerase
    • OneTaq® Hot Start 2X Master Mix with GC Buffer
    • OneTaq® Hot Start 2X Master Mix with Standard Buffer
    • OneTaq® Hot Start DNA Polymerase
    • OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

  • 注意事项
    1. Product specifications for individual components in the OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer are available separately.
    2. OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer is stable for fifteen freeze-thaw cycles when stored at -20°C.
    3. OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer is also stable for one month at 4°C, so for frequent use, an aliquot may be kept at 4°C.

  • 参考文献
    1. Barnes, W.M. (1994). Proc. Natl. Acad. Sci. USA. 91, 2216-2220.
    2. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
    3. Powell, L.M. et al. (1987). Cell. 50, 831-840.

操作说明、说明书 & 用法

  • 操作说明
    1. Protocol for OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer
    2. Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases

  • 使用指南
    • Activity of Restriction Enzymes in PCR Buffers
    • Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases

  • 应用实例
    • Robust Colony PCR from Multiple E. coli Strains using OneTaq® Quick-Load® Master Mixes

工具 & 资源

  • Web 工具
    • Tm Calculator

FAQs & 问题解决指南

  • FAQs
    1. How should I set up a PCR using the OneTaq® Hot Start Master Mixes?
    2. Can I use my regular Taq-based cycling conditions for OneTaq® Hot Start DNA Polymerase based products?
    3. How do I activate OneTaq® Hot Start Polymerase?
    4. What are the stability and storage requirements of the Quick-Load® OneTaq® Master Mixes?
    5. What type of DNA ends result from a primer extension reaction or a PCR using OneTaq® DNA Polymerase?
    6. How long a product can be made by OneTaq® DNA Polymerase?
    7. Can OneTaq® DNA Polymerase be used with uracil-containing primers or bisulfite-treated DNA?
    8. What is the fidelity of OneTaq® DNA Polymerase?
    9. When should I add the High GC Enhancer?
    10. Where can I find help troubleshooting my PCR?
    11. How should I determine an appropriate annealing temperature for my reaction?
    12. How is OneTaq DNA Polymerase different from LongAmp™ Taq DNA Polymerase?

  • 实验技巧
    A master mix with a gel loading dye is the ultimate time-saver! It allows for easy reaction set up and with the gel loading dye already in the mix, the results of your PCR are available even faster.

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

  • 专题文章
    • Using aptamers to control enzyme activities: Hot Start Taq and beyond

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • M0489S_L_v1
    • M0489S_L_v2

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • M0489S_L_v1_0271706
    • M0489S_L_v1_0281712
    • M0489L_v1_10012825
    • M0489S_v1_10012555
    • M0489L_v1_10014225
    • M0489S_v1_10014224
    • M0489L_v1_10017659
    • M0489L_v1_10009605
    • M0489S_v1_10029613
    • M0489S_v1_10033580
    • M0489L_v1_10027511
    • M0489S_v1_10036260
    • M0489L_v1_10036261
    • M0489S_v1_10044967
    • M0489L_v1_10044966
    • M0489L_v1_10050004
    • M0489S_v1_10050005
    • M0489L_v1_10053241
    • M0489S_v1_10053240
    • M0489L_v1_10054255
    • M0489S_v1_10055340
    • M0489L_v1_10057970
    • M0489S_v1_10060657
    • M0489S_v1_10062229
    • M0489L_v1_10062228
    • M0489L_v2_10068191
    • M0489S_v2_10068190
    • M0489L_v2_10075712
    • M0489L_v2_10082801
    • M0489L_v2_10092014
    • M0489L_v2_10098039
    • M0489S_v2_10103240
    • M0489S_v2_10104877
    • M0489L_v2_10105615
    • M0489S_v2_10108356
    • M0489L_v2_10112139
    • M0489L_v2_10112546
    • M0489S_v2_10112454
    • M0489L_v2_10112761
    • M0489L_v2_10117612
    • M0489S_v2_10119635
    • M0489L_v2_10119755
    • M0489L_v2_10122516
    • M0489L_v2_10130738
    • M0489L_v2_10138875
    • M0489S_v2_10145963
    • M0489L_v2_10145247
    • M0489L_v2_10159514
    • M0489L_v2_10161054

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer
    • OneTaq® High GC Enhancer