OneTaq® Hot Start DNA Polymerase |NEB酶试剂 New England Biolabs

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OneTaq® Hot Start DNA Polymerase

The ONE polymerase for your endpoint PCR needs

OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases for use with routine and difficult PCR experiments.

  • Unique hot start formulation eliminates non-specific amplification and does not require a separate activation step
  • Robust yield with minimal optimization
  • Ideal for routine, AT- or GC-rich templates
  • Room temperature reaction setup

精选视频

OneTaq® Hot Start DNA Polymerase |

How to Amplify GC-rich DNA

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库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0481S 5,000 units/ml 200 units ¥889.00 有 有 无 无 无
M0481L 5,000 units/ml 1,000 units ¥3,559.00 有 无 无 无 无
M0481X 5,000 units/ml 5,000 units ¥14,209.00 无 无 无 无 无
如何订购
Please enter a quantity for at least one size

OneTaq® Hot Start DNA Polymerase | Want to try this product? Request a sample

产品信息

OneTaq® Hot Start DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases combined with an aptamer-based inhibitor. The 3´→ 5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1) and the hot start formulation combines convenience with decreased interference from primer dimers and secondary products. The OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template’s GC content.

The inhibitor binds reversibly, blocking polymerase activity at temperatures below 45°C. The polymerase is activated during normal cycling conditions, allowing reactions to be set up at room temperature. OneTaq Hot Start DNA Polymerase does not require a separate high temperature incubation step to activate the enzyme and can be used in typical Taq-based cycling protocols.

OneTaq Hot Start DNA Polymerase is supplied with two 5X buffers: (Standard and GC), as well as a High GC Enhancer solution. For most routine and/or AT-rich amplicons (Lambda, etc.) or complex amplicons with up to ~65% GC content, OneTaq Standard Reaction Buffer provides robust amplification. For GC-rich amplicons, the OneTaq GC Reaction Buffer can improve both performance and yield. For particularly high GC or difficult amplicons (i.e. ≥ 75% GC), the OneTaq High GC Enhancer can be added at a final concentration of 10–20% to reactions containing OneTaq GC Reaction Buffer.

Comparison of OneTaq Products to Other Commercially Available Polymerases

OneTaq® Hot Start DNA Polymerase | 

Amplification of a selection of high GC human genomic DNA targets demonstrates OneTaq performance. All polymerases were cycled according to manufacturer’s recommendations, including the use of additives to enhance the amplification of targets with high GC content. Yield (dot size) and purity (color) of reaction product were quantified from triplicate reactions on a Perkin Elmer LabChip®. A large, dark green dot represents the highest yield and purity.
OneTaq® Hot Start DNA Polymerase |

产品来源

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent® DNA Polymerase gene from Pyrococcus species GB-D.

产品类别:
OneTaq® DNA Polymerases Products,
Taq DNA Polymerase Products

应用:
Hot Start PCR,
Multiplex PCR,
Specialty PCR,

Routine PCR,

PCR

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0481S     -20    
        OneTaq® Hot Start DNA Polymerase M0481SVIAL -20 1 x 0.04 ml 5,000 units/ml
        OneTaq® Standard Reaction Buffer Pack B9022SVIAL -20 1 x 2 ml 5 X
        OneTaq® GC Reaction Buffer Pack B9023SVIAL -20 1 x 2 ml 5 X
        OneTaq® High GC Enhancer B9026AVIAL -20 1 x 2 ml 不适用
    • M0481L     -20    
        OneTaq® Hot Start DNA Polymerase M0481LVIAL -20 1 x 0.2 ml 5,000 units/ml
        OneTaq® Standard Reaction Buffer Pack B9022SVIAL -20 5 x 2 ml 5 X
        OneTaq® GC Reaction Buffer Pack B9023SVIAL -20 3 x 2 ml 5 X
        OneTaq® High GC Enhancer B9026AVIAL -20 3 x 2 ml 不适用
    • M0481X     -20    
        OneTaq® Hot Start DNA Polymerase M0481LVIAL -20 5 x 0.2 ml 5,000 units/ml
        OneTaq® Standard Reaction Buffer Pack B9022SVIAL -20 25 x 2 ml 5 X
        OneTaq® GC Reaction Buffer Pack B9023SVIAL -20 15 x 2 ml 5 X
        OneTaq® High GC Enhancer B9026AVIAL -20 15 x 2 ml 不适用

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.

    反应条件

    1X OneTaq® Standard Reaction Buffer Pack

    1X OneTaq® Standard Reaction Buffer Pack
    20 mM Tris-HCl
    22 mM NH4Cl
    22 mM KCl
    1.8 mM MgCl2
    0.06% IGEPAL® CA-630
    0.05% Tween® 20
    (pH 8.9 @ 25°C)

    使用浓度

    1.25 units/50μl reaction

    贮存溶液

    10 mM Tris-HCl
    100 mM KCl
    1 mM DTT
    0.1 mM EDTA
    50% Glycerol
    0.5% Tween® 20
    0.5% IGEPAL® CA-630
    pH 7.4 @ 25°C

    热失活

    否

    5′ – 3′ 核酸外切酶

    Yes

    3′ – 5′ 核酸外切酶

    Yes

    链置换

    No

    单位活性检测条件

    1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA. 

  • 优势和特性

    应用特性

    • High Sensitivity PCR
    • High Throughput PCR
    • Routine PCR
    • GC-rich PCR
    • AT-rich PCR
    • Colony PCR
    • Long PCR (up to ~6 kb genomic

  • 相关产品

    相关产品

    • Deoxynucleotide (dNTP) Solution Set
    • Deoxynucleotide (dNTP) Solution Mix
    • Magnesium Chloride (MgCl2) Solution
    • Magnesium Sulfate (MgSO4) Solution
    • OneTaq® DNA Polymerase

    单独销售的组分

    • OneTaq® Standard Reaction Buffer Pack
    • OneTaq® GC Reaction Buffer Pack

  • 注意事项
    1. The OneTaq High GC Enhancer should not be used alone. It should be added only to the OneTaq GC Reaction Buffer and will typically enhance yields when other conditions have failed.
    2. Product specifications for individual components in the OneTaq Hot Start DNA Polymerase mix are available separately.

  • 参考文献
    1. Barnes, W.M. (1994). Proc. Natl. Acad. Sci. USA. 91, 2216-2220.
    2. Saiki R.K. et al. (1985). Science. 230, 1530-1554.
    3. Powell, L.M. et al. (1987). Cell. 50, 831-840.

操作说明、说明书 & 用法

  • 操作说明
    1. Protocol for OneTaq Hot Start DNA Polymerase (M0481)

  • 使用指南
    • Activity of Restriction Enzymes in PCR Buffers
    • Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases
    • Guidelines for PCR Optimization with Thermophilic DNA Polymerases

  • 应用实例
    • Robust Colony PCR from Multiple E. coli Strains using OneTaq® Quick-Load® Master Mixes

工具 & 资源

  • 选择指南
    • DNA Polymerase Selection Chart
    • Thermophilic DNA Polymerases

  • Web 工具
    • Tm Calculator

FAQs & 问题解决指南

  • FAQs
    1. How should I set up a PCR using OneTaq® Hot Start DNA Polymerase?
    2. Can I use my regular Taq-based cycling conditions for OneTaq® DNA Polymerase based products?
    3. Which buffer should I use?
    4. How do I activate OneTaq® Hot Start Polymerase?
    5. Is OneTaq® Hot Start DNA Polymerase active in other Taq reaction buffers?
    6. When should I add the High GC Enhancer?
    7. Can OneTaq® DNA Polymerase be used in colony PCR?
    8. What type of DNA ends result from a primer extension reaction or a PCR using OneTaq® DNA Polymerase?
    9. How long a product can be made by OneTaq® DNA Polymerase?
    10. Can OneTaq® DNA Polymerase be used with uracil-containing primers or bisulfite-treated DNA?
    11. What is the fidelity of OneTaq® DNA Polymerase?
    12. Which buffer should I use if I want to control the level of magnesium in the reaction?
    13. Where can I find help troubleshooting my PCR?
    14. How should I determine an appropriate annealing temperature for my reaction?
    15. How is OneTaq DNA Polymerase different from LongAmp™ Taq DNA Polymerase?

  • 问题解决指南
    • PCR Troubleshooting Guide

  • 实验技巧
    Did you know most OneTaq Hot Start reactions amplify more efficiently and robustly when you use a 68°C extension temperature?

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    OneTaq® Hot Start DNA Polymerase | Powered by Bioz See more details on Bioz

  • 专题文章
    • Using aptamers to control enzyme activities: Hot Start Taq and beyond

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • M0481S_L_X_v1
    • M0481S_L_X_v2

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • M0481S_L_X_v1_0101512
    • M0481S_L_X_v1_0101606
    • M0481S_L_X_v1_0101612
    • M0481S_L_X_v1_0101706
    • M0481S_L_X_v1_0101712
    • M0481L_v1_10012698
    • M0481S_v1_10012672
    • M0481X_v1_10014190
    • M0481X_v1_10018226
    • M0481L_v1_10019278
    • M0481X_v1_10019277
    • M0481G_v0_10010785
    • M0481X_v1_10023116
    • M0481L_v1_10027986
    • M0481X_v1_10027987
    • M0481X_v1_10028225
    • M0481S_v1_10030724
    • M0481X_v1_10032797
    • M0481X_v1_10034640
    • M0481L_v1_10034534
    • M0481X_v1_10038373
    • M0481G_v0_10040424
    • M0481L_v1_10035625
    • M0481X_v1_10039159
    • M0481X_v1_10042309
    • M0481L_v1_10042310
    • M0481X_v1_10042687
    • M0481X_v1_10047068
    • M0481L_v1_10048422
    • M0481L_v1_10048747
    • M0481X_v1_10048735
    • M0481S_v1_10051085
    • M0481L_v1_10054104
    • M0481X_v1_10054103
    • M0481S_v1_10054098
    • M0481X_v1_10057356
    • M0481S_v1_10057355
    • M0481S_v1_10061082
    • M0481L_v1_10060135
    • M0481X_v1_10060860
    • M0481G_v0_10060005
    • M0481L_v1_10062271
    • M0481L_v1_10063190
    • M0481X_v1_10062270
    • M0481L_v1_10064519
    • M0481X_v1_10063898
    • M0481L_v2_10065923
    • M0481X_v2_10065924
    • M0481X_v2_10068534
    • M0481L_v2_10070052
    • M0481X_v2_10070051
    • M0481X_v2_10071139
    • M0481S_v2_10068671
    • M0481L_v2_10072336
    • M0481X_v2_10073013
    • M0481L_v2_10074869
    • M0481X_v2_10074870
    • M0481X_v2_10079006
    • M0481L_v2_10082438
    • M0481X_v2_10088678
    • M0481L_v2_10092409
    • M0481X_v2_10096732
    • M0481X_v2_10097058
    • M0481L_v2_10096733
    • M0481S_v2_10095635
    • M0481X_v2_10098356
    • M0481L_v2_10101200
    • M0481X_v2_10101201
    • M0481G_v0_10103258
    • M0481L_v2_10105512
    • M0481X_v2_10105513
    • M0481L_v2_10115544
    • M0481X_v2_10114378
    • M0481G_v0_10119920
    • M0481S_v2_10118631
    • M0481G_v0_10120891
    • M0481L_v2_10117179
    • M0481X_v2_10117180
    • M0481L_v2_10120993
    • M0481X_v2_10120994
    • M0481G_v0_10127405
    • M0481L_v2_10129099
    • M0481X_v2_10129100
    • M0481L_v2_10132263
    • M0481X_v2_10134799
    • M0481L_v2_10138006
    • M0481X_v2_10138005
    • M0481G_v1_10142489
    • M0481L_v2_10139838
    • M0481X_v2_10139839
    • M0481L_v2_10144877
    • M0481S_v2_10141008
    • M0481X_v2_10144876
    • M0481L_v2_10148034
    • M0481G_v1_10149467
    • M0481G_v1_10157100
    • M0481G_v1_10158544
    • M0481S_v2_10160179
    • M0481L_v2_10156027
    • M0481X_v2_10148700

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • OneTaq® Hot Start DNA Polymerase
    • OneTaq® High GC Enhancer