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OneTaq® 2X Master Mix with Standard Buffer
OneTaq® DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases for use with routine and difficult PCR experiments.
Obtain high yields across a wide range of AT / GC content
2X higher fidelity than Taq
Master mix is a 2X concentrated solution containing everything needed for robust amplification
精选视频
How to Amplify GC-rich DNA
观看其他视频
产品信息
OneTaq® 2X Master Mix with Standard Buffer is an optimized blend of Taq and Deep Vent® DNA Polymerases ideally suited to routine PCR applications from a variety of templates including pure DNA solutions, bacterial colonies, and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The convenient master mix formulation contains dNTPs, MgCl2, buffer components and stabilizers, requiring only the addition of primers and DNA template for robust amplification.
Comparison of OneTaq Products to Other Commercially Available Polymerases
Amplification of a selection of high GC human genomic DNA targets demonstrates OneTaq performance. All polymerases were cycled according to manufacturer’s recommendations, including the use of additives to enhance the amplification of targets with high GC content. Yield (dot size) and purity (color) of reaction product were quantified from triplicate reactions on a Perkin Elmer LabChip®. A large, dark green dot represents the highest yield and purity.
产品来源
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent® DNA Polymerase gene from Pyrococcus species GB-D.
产品类别:
OneTaq® DNA Polymerases Products,
Taq DNA Polymerase Products,
Master Mixes Products
应用:
Fast PCR,
Multiplex PCR,
Specialty PCR,
Routine PCR,
PCR
产品组分信息
产品组分信息
本产品提供以下试剂或组分：
NEB #
名称
组分货号
储存温度
数量
浓度
M0482S
-20
OneTaq® 2X Master Mix with Standard Buffer
M0482SVIAL
-20
2 x 1.25 ml
2 X
M0482L
-20
OneTaq® 2X Master Mix with Standard Buffer
M0482SVIAL
-20
10 x 1.25 ml
2 X
特性和用法
1X 预混液组分
20 mM Tris-HCl 22 mM KCl 22 mM NH4Cl 1.8 mM MgCl2 5% Glycerol 0.05% Tween® 20 0.06% IGEPAL® CA-630 0.2 mM dNTPs 25 units/ml OneTaq® DNA Polymerase pH 8.9 @ 25°C
热失活
否
单位活性检测条件
1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.
优势和特性
应用特性
GC-rich PCR
High Sensitivity PCR
High Throughput PCR
Colony PCR
Long PCR (up to ~6 kb genomic)
AT-rich PCR
相关产品
相关产品
Magnesium Chloride (MgCl2) Solution
OneTaq® DNA Polymerase
OneTaq® Hot Start DNA Polymerase
OneTaq® Quick-Load® 2X Master Mix with Standard Buffer
OneTaq® Hot Start 2X Master Mix with Standard Buffer
注意事项
OneTaq 2X Master Mix with Standard Buffer is stable for fifteen freeze-thaw cycles when stored at -20°C.
OneTaq 2X Master Mix with Standard Buffer is also stable for one month at 4°C, so for frequent use, an aliquot may be kept at 4°C.
Product specifications for individual components in the OneTaq 2X Master Mix with Standard Buffer are available separately.
参考文献
Barnes,W.M. (1994). Proc. Natl Acad. Sci. USA. 91, 2216-2220.
Saiki,R.K. et al (1985). Science. 230, 1350-1354.
Powell,L.M. et. al. (1987). Cell. 50, 831-840.
Sun,y., Hegamyer, G. and Colburn,N. (1993). Biotechniques. 15, 372-374.
Sarker, G., Kapelner, S. and Summer, S.S. (1990). Nuclear Acids Res.. 18, 7465.
Magda Matoušková, Pavel Veselý, Petr Daniel, Giada Mattiuzzo, Ralph Hector, Linda Scobie, Yasuhiro Takeuchi and Jiří Hejnar (2013). The Role of DNA Methylation in Expression and Transmission of Procine Endogenous Retrovirus. Journal of Virology. JVI.03262-12,
操作说明、说明书 & 用法
操作说明
Protocol for OneTaq® 2X Master Mix with Standard Buffer (M0482)
使用指南
Activity of Restriction Enzymes in PCR Buffers
Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases
Guidelines for PCR Optimization with Thermophilic DNA Polymerases
应用实例
Robust Colony PCR from Multiple E. coli Strains using OneTaq® Quick-Load® Master Mixes
工具 & 资源
选择指南
DNA Polymerase Selection Chart
Thermophilic DNA Polymerases
Web 工具
Tm Calculator
FAQs & 问题解决指南
FAQs
How should I set up a PCR using the OneTaq® Master Mixes?
Can I use my regular Taq-based cycling conditions for OneTaq® DNA Polymerase based products?
Can I use OneTaq® DNA Polymerase in “hot start” PCR to help with specificity or primer dimer problems?
What are the stability and storage requirements of the OneTaq® Master Mixes?
Can OneTaq® DNA Polymerase be used in colony PCR?
What type of DNA ends result from a primer extension reaction or a PCR using OneTaq® DNA Polymerase?
How long a product can be made by OneTaq® DNA Polymerase?
Can OneTaq® DNA Polymerase be used with uracil-containing primers or bisulfite-treated DNA?
What is the fidelity of OneTaq® DNA Polymerase?
Where can I find help troubleshooting my PCR?
How should I determine an appropriate annealing temperature for my reaction?
How is OneTaq DNA Polymerase different from LongAmp™ Taq DNA Polymerase?
问题解决指南
PCR Troubleshooting Guide
实验技巧
Use OneTaq 2X Master Mix with Standard Buffer to amplify routine targets with up to 65% GC content.
引用 & 技术文献
引用文献
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更多引用文献
Stöhr AC, López-Bueno A, Blahak S, Caeiro MF, Rosa GM, Alves de Matos AP, Martel A, Alejo A, Marschang RE (2015) Phylogeny and differentiation of reptilian and amphibian ranaviruses detected in europe PLoS One; 10(2), e0118633. PubMedID: 25706285, DOI: 10.1371/journal.pone.0118633
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