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Q5® High-Fidelity DNA Polymerase
Fidelity at its finest.
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515).
Highest fidelity amplification (~280X higher than Taq)
Ultra-low error rates
Superior performance for a broad range of amplicons (from high AT to high GC)
Hot start and master mix formats available
精选视频
Important Tips for Q5® Polymerase
观看其他视频
Want to try this product? Request a sample Interested in glycerol-free or custom formulations? – contact our Custom Solutions Group
Bulk packaging may also be available and requested for large recurring orders. Learn More
产品信息
Q5® High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.
Amplification of a variety of human genomic amplicons from low to high GC content using Q5 High-Fidelity DNA Polymerase. All reactions were conducted using 30 cycles of amplification and visualized by microfluidic LabChip® analysis.
产品来源
An E. coli strain that carries the Q5 High-Fidelity DNA Polymerase gene.
产品类别:
Q5® High-Fidelity DNA Polymerases Products
应用:
Gibson Assembly®,
Long Range PCR,
Fast PCR,
High-Fidelity PCR,
Multiplex PCR,
DNA Amplification, PCR & qPCR,
Specialty PCR,
Routine PCR,
PCR,
Fast Cloning: Accelerate your cloning workflows with reagents from NEB
产品组分信息
产品组分信息
本产品提供以下试剂或组分：
NEB #
名称
组分货号
储存温度
数量
浓度
M0491V
-20
Q5® High-Fidelity DNA Polymerase
M0491VVIAL
-20
1 x 0.025 ml
2,000 units/ml
Q5® Reaction Buffer Pack
B9027SVIAL
-20
1 x 1.5 ml
5 X
Q5® High GC Enhancer
B9028AVIAL
-20
1 x 1.5 ml
5 X
M0491S
-20
Q5® High-Fidelity DNA Polymerase
M0491SVIAL
-20
1 x 0.05 ml
2,000 units/ml
Q5® Reaction Buffer Pack
B9027SVIAL
-20
1 x 1.5 ml
5 X
Q5® High GC Enhancer
B9028AVIAL
-20
1 x 1.5 ml
5 X
M0491L
-20
Q5® High-Fidelity DNA Polymerase
M0491LVIAL
-20
1 x 0.25 ml
2,000 units/ml
Q5® Reaction Buffer Pack
B9027SVIAL
-20
4 x 1.5 ml
5 X
Q5® High GC Enhancer
B9028AVIAL
-20
4 x 1.5 ml
5 X
特性和用法
单位定义
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 74°C.
单位活性检测条件
25 mM TAPS-HCl (pH 9.3 @ 25°C), 50 mM KCl, 2 mM MgCl2, 1 mM β-mercaptoethanol, 200 μM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.
优势和特性
应用特性
High-fidelity PCR
Cloning
Long or Difficult Amplification
High-throughput PCR
相关产品
相关产品
Deoxynucleotide (dNTP) Solution Mix
Deoxynucleotide (dNTP) Solution Set
Q5® Hot Start High-Fidelity 2X Master Mix
Q5® High-Fidelity 2X Master Mix
Q5® Hot Start High-Fidelity DNA Polymerase
Q5® Reaction Buffer Pack
操作说明、说明书 & 用法
操作说明
PCR Using Q5® High-Fidelity DNA Polymerase (M0491)
使用指南
Activity of Restriction Enzymes in PCR Buffers
Guidelines for PCR Optimization with Thermophilic DNA Polymerases
应用实例
AppNote_Multiplex_PCR_Using_Q5_HF_DNA_Polymerase
工具 & 资源
选择指南
DNA Polymerase Selection Chart
Web 工具
Tm Calculator
FAQs & 问题解决指南
FAQs
What are the advantages to using Q5® High-Fidelity DNA Polymerase?
What is the fidelity of Q5® High-Fidelity DNA Polymerase?
How should I determine an appropriate annealing temperature for my reaction?
What should my primer concentration be when using Q5® High-Fidelity DNA Polymerase products?
How should I set up a PCR experiment using Q5® High-Fidelity DNA Polymerase?
My template is GC rich or supercoiled. How can I optimize my product yield using Q5® High-Fidelity DNA Polymerase?
Do I need to modify my annealing temperature when using the Q5® High GC Enhancer?
When should I add the High GC Enhancer?
Are the DNA fragments produced by Q5® High-Fidelity DNA Polymerase blunt-ended or do they have the single-base 3´ overhang that Taq DNA Polymerase yields?
There is a precipitate in the bottom of the buffer tube. Is this normal?
What length of product can be made by Q5® High-Fidelity DNA Polymerase?
I am having trouble amplifying a template that is longer than 5kb. How can I optimize my product yield using Q5® High-Fidelity DNA Polymerase?
Does Q5® High-Fidelity DNA Polymerase exhibit a strand displacement activity?
Where can I find help troubleshooting my PCR?
Will Q5® High-Fidelity DNA Polymerase incorporate dUTPs?
I’d like to clone a fragment amplified with Q5® High-Fidelity DNA Polymerase. Do I have to blunt-end clone?
Do other polymerases work in Q5® Reaction Buffer?
I am competing in the iGEM competition. Do you have any products that I should consider from NEB?
What is the difference between Q5 and Q5U Hot Start High-Fidelity DNA Polymerase?
问题解决指南
PCR Troubleshooting Guide
引用 & 技术文献
引用文献
产品引用文献查找工具
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Harish Nag Kankipati, Marta Rubio-Texeira, Dries Castermans, George Diallinas, Johan M Thevelein (2015) Sul1 and Sul2 Sulfate Transceptors Signal to Protein Kinase A upon Exit of Sulfur Starvation. J Biol Chem; 290, 10430-46. PubMedID: 25724649, DOI: 10.1074/jbc.M114.629022
Amin Zargar, David N Quan, Milad Emamian, Chen Yu Tsao, Hsuan-Chen Wu, Chelsea R Virgile, William E Bentley (2015) Rational design of ‘controller cells’ to manipulate protein and phenotype expression. Metab Eng; , PubMedID: 25908186, DOI: 10.1016/j.ymben.2015.04.001
Yonghe Zhang, Huiming Huang, Shanshan Xu, Bo Wang, Jianhua Ju, Huarong Tan, Wenli Li (2015) Activation and enhancement of Fredericamycin A production in deepsea-derived Streptomyces somaliensis SCSIO ZH66 by using ribosome engineering and response surface methodology. Microb Cell Fact; 14, 64. PubMedID: 25927229, DOI: 10.1186/s12934-015-0244-2
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Jun Wu, Daiji Okamura, Mo Li, Keiichiro Suzuki, Chongyuan Luo, Li Ma, Yupeng He, Zhongwei Li, Chris Benner, Isao Tamura, Marie N Krause, Joseph R Nery, Tingting Du, Zhuzhu Zhang, Tomoaki Hishida, Yuta Takahashi, Emi Aizawa, Na Young Kim, Jeronimo Lajara, Pedro Guillen, Josep M Campistol, Concepcion Rodriguez Esteban, Pablo J Ross, Alan Saghatelian, Bing Ren, Joseph R Ecker, Juan Carlos Izpisua Belmonte (2015) An alternative pluripotent state confers interspecies chimaeric competency. Nature; , PubMedID: 25945737, DOI: 10.1038/nature14413
Longhai Dai, Can Liu, Yueming Zhu, Jiangsheng Zhang, Yan Men, Zeng Yan, Yuanxia Sun (2015) Functional Characterization of Cucurbitadienol Synthase and Triterpene Glycosyltransferase Involved in Biosynthesis of Mogrosides from Siraitia grosvenorii. Plant Cell Physiol; , PubMedID: 25759326, DOI: 10.1093/pcp/pcv043
Yang YJ, Han YY, Chen K, Zhang Y, Liu X, Li S, Wang KQ, Ge JB, Liu W, Zuo J. (2015) TonEBP modulates the protective effect of taurine in ischemia-induced cytotoxicity in cardiomyocytes Cell Death Dis; PubMedID: 26673669, DOI: 10.1038
Yuan Xue, Jossef Osborn, Anand Panchal, Jay L Mellies (2015) The RpoE Stress Response Pathway Mediates Reduction of the Virulence of Enteropathogenic Escherichia coli by Zinc. Appl Environ Microbiol; 81, 3766-74. PubMedID: 25819956, DOI: 10.1128/AEM.00507-15
Christine Henke, Pamela L Strissel, Maria-Theresa Schubert, Megan Mitchell, Claus C Stolt, Florian Faschingbauer, Matthias W Beckmann, Reiner Strick (2015) Selective expression of sense and antisense transcripts of the sushi-ichi-related retrotransposon – derived family during mouse placentogenesis. Retrovirology; 12, 9. PubMedID: 25888968, DOI: 10.1186/s12977-015-0138-8
Binyamin D Berkovits, Christine Mayr (2015) Alternative 3′ UTRs act as scaffolds to regulate membrane protein localization. Nature; , PubMedID: 25896326, DOI: 10.1038/nature14321
Wang XJ, Zhang XJ, Hu W, Zhang TY, Wang SQ (2014) A simple and efficient strategy for the de novo construction of greater-than-genome-length hepatitis B virus replicons J Virol Methods; 207, 158-62. PubMedID: 25025817, DOI: 10.1016/j.jviromet.2014.07.009
Vidhyadhar Nandana, Sushant Singh, Abhay Narayan Singh, Vikash Kumar Dubey (2014) Procerain B, a cysteine protease from Calotropis procera, requires N-terminus pro-region for activity: cDNA cloning and expression with pro-sequence. Protein Expr Purif; 103C, 16-22. PubMedID: 25173974, DOI: 10.1016/j.pep.2014.08.003
Martin Kostovcik, Craig C Bateman, Miroslav Kolarik, Lukasz L Stelinski, Bjarte H Jordal, Jiri Hulcr (2014) The ambrosia symbiosis is specific in some species and promiscuous in others: evidence from community pyrosequencing. ISME J; , PubMedID: 25083930, DOI: 10.1038/ismej.2014.115
Bert De Rybel, Milad Adibi, Alice S. Breda, Jos R. Wendrich, Margot E. Smit, Ondej Novk, Nobutoshi Yamaguchi, Saiko Yoshida, Gert Van Isterdael, Joakim Palovaara, Bart Nijsse, Mark V. Boekschoten, Guido Hooiveld, Tom Beeckman, Doris Wagner, Karin Ljung, Christian Fleck, Dolf Weijers (2014) Integration of growth and patterning during vascular tissue formation in Arabidopsis Science; 345, 1255215. PubMedID: 25104393, DOI: 10.1126/science.1255215
Xin Duan, Arjun Krishnaswamy, Irina De la Huerta, Joshua R Sanes (2014) Type II Cadherins Guide Assembly of a Direction-Selective Retinal Circuit. Cell; 158, 793-807. PubMedID: 25126785, DOI: 10.1016/j.cell.2014.06.047
Lieve Naesens, Luke Guddat, Dianne Keough, André B.P. van Kuilenburg, Judith Meijer, Johan Vande Voorde and Jan Balzarini (2013) ROLE OF HUMAN HYPOXANTHINE GUANINE PHOSPHORIBOSYLTRANSFERASE IN ACTIVATION OF THE ANTIVIRAL AGENT T-705 (FAVIPIRAVIR) Mol Pharmacol; 87247. PubMedID: 23907213
Anastassia Voronova Erin Coyne, Ashraf Al Madhoun, Joel V. Fair, Neven Bosiljcic, Catherine St-Louis, Grace Li, Sherry Thurig, Valerie A. Wallace, Nadine Wiper-Bergeron, and Ilona S. Skerjanc (2013) Hedgehog Signaling Regulates MyoD Expression and Activity J Biol Chem; 288(6), 4389–4404. PubMedID: 23266826
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专题文章
Polymerase Fidelity: What is it, and what does it mean for your PCR?
Want to try this product? Request a sample 
Interested in glycerol-free or custom formulations? – contact our Custom Solutions Group 
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