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Q5® High-Fidelity 2X Master Mix
Fidelity at its finest
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515).
Highest fidelity amplification (~280X higher than Taq)
Ultra-low error rates
Superior performance for a broad range of amplicons (from high AT to high GC)
Hot start and master mix formats available
精选视频
Important Tips for Q5® Polymerase
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Bulk packaging may also be available and requested for large recurring orders. Learn More
产品信息
The Q5 High-Fidelity 2X Master Mix offers robust, high-fidelity performance in a convenient master mix format. The Q5 High- Fidelity 2X Master Mix features a high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. The convenient master mix formulation is supplied at a 2X concentration. The mix contains dNTPs, Mg++ and a proprietary broad-use buffer requiring only the addition of primers and DNA template for robust amplification regardless of GC content. When used at the recommended 1X final concentration, the Q5 High-Fidelity Master Mix contains 2 mM Mg++. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.
产品来源
An E. coli strain that carries the Q5 High-Fidelity DNA Polymerase gene.
产品类别:
Q5® High-Fidelity DNA Polymerases Products,
Master Mixes Products
应用:
Long Range PCR,
Fast PCR,
High-Fidelity PCR,
Multiplex PCR,
DNA Amplification, PCR & qPCR,
Specialty PCR,
Routine PCR,
PCR,
Fast Cloning: Accelerate your cloning workflows with reagents from NEB
产品组分信息
产品组分信息
本产品提供以下试剂或组分:
NEB #
名称
组分货号
储存温度
数量
浓度
M0492S
-20
Q5® High-Fidelity 2X Master Mix
M0492SVIAL
-20
2 x 1.25 ml
2 X
M0492L
-20
Q5® High-Fidelity 2X Master Mix
M0492SVIAL
-20
10 x 1.25 ml
2 X
特性和用法
热失活
否
优势和特性
应用特性
High-fidelity PCR
Cloning
Long or Difficult Amplification
High-throughput PCR
相关产品
相关产品
Deoxynucleotide (dNTP) Solution Mix
Deoxynucleotide (dNTP) Solution Set
Q5® High-Fidelity DNA Polymerase
Q5® Hot Start High-Fidelity 2X Master Mix
Q5® Hot Start High-Fidelity DNA Polymerase
Q5® Reaction Buffer Pack
注意事项
A precipitate (most noticeable after the first 1–2 freeze/thaw cycles) is not uncommon. To ensure optimal performance, the master mix should be thawed and resuspended prior to use. Stability testing using up to 15 freeze/thaw cycles has shown no negative effect on master mix performance.
Product specifications for individual components in the Q5 High-Fidelity 2X Master Mix are available separately.
参考文献
Anastassia Voronova Erin Coyne, Ashraf Al Madhoun, Joel V. Fair, Neven Bosiljcic, Catherine St-Louis, Grace Li, Sherry Thurig, Valerie A. Wallace, Nadine Wiper-Bergeron, and Ilona S. Skerjanc. (2013). Hedgehog Signaling Regulates MyoD Expression and Activity. J Biol Chem. 288(6), 4389–4404. PubMedID: PMC3567689
Lieve Naesens, Luke Guddat, Dianne Keough, André B.P. van Kuilenburg, Judith Meijer, Johan Vande Voorde and Jan Balzarini (2013). ROLE OF HUMAN HYPOXANTHINE GUANINE PHOSPHORIBOSYLTRANSFERASE IN ACTIVATION OF THE ANTIVIRAL AGENT T-705 (FAVIPIRAVIR). Molecular Pharmacology Fast Forward. 87247,
Hicham Bouabe, Klaus Okkenhaug (2013). A Protocol for Construction of Gene Targeting Vectors and Generation of Homologous Recombinant Embryonic Stem Cells. Methods in Molecular Biology. 1064, 337-354.
操作说明、说明书 & 用法
操作说明
Protocol for Q5® High-Fidelity 2X Master Mix
使用指南
Activity of Restriction Enzymes in PCR Buffers
Guidelines for PCR Optimization with Thermophilic DNA Polymerases
工具 & 资源
选择指南
DNA Polymerase Selection Chart
Web 工具
Tm Calculator
FAQs & 问题解决指南
FAQs
What are the advantages to using Q5® High-Fidelity 2X Master Mix?
What is the fidelity of Q5® High-Fidelity DNA Polymerase?
How should I determine an appropriate annealing temperature for my reaction?
How should I set up a PCR experiment using Q5® High-Fidelity 2X Master Mix?
What should my primer concentration be when using Q5® High-Fidelity DNA Polymerase products?
My template is GC rich or supercoiled. How can I optimize my product yield using Q5® High-Fidelity Master Mixes?
There is a precipitate in the bottom of the Master Mix tube? Is this normal?
Are the DNA fragments produced by Q5® High-Fidelity DNA Polymerase blunt-ended or do they have the single-base 3´ overhang that Taq DNA Polymerase yields?
What length of product can be made by Q5® High-Fidelity DNA Polymerase?
I have a tube of the Q5® High GC Enhancer from another product formulation – can I add it to the Q5 Master Mix?
What are the stability and storage requirements of the Q5® Master Mixes?
Does Q5® High-Fidelity DNA Polymerase exhibit a strand displacement activity?
Where can I find help troubleshooting my PCR?
I’d like to clone a fragment amplified with Q5® High-Fidelity DNA Polymerase. Do I have to blunt-end clone?
Will Q5® High-Fidelity DNA Polymerase incorporate dUTPs?
问题解决指南
PCR Troubleshooting Guide
引用 & 技术文献
引用文献
产品引用文献查找工具
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专题文章
Polymerase Fidelity: What is it, and what does it mean for your PCR?